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Detection of Hsp70 protein in plants exposed to various stress factors.
Lengálová, Alžběta ; Hýsková, Veronika (advisor) ; Liberda, Jiří (referee)
! Plants are continuously exposed to various stress conditions. Being sessile, they are not able to escape from adverse conditions. Therefore, they have developed specific defence mechanisms. Most studies focus on plant responses to a single type of stress. However, plants in nature must cope with a variety of stresses at the same time. In this work, the effects of heat shock on the interaction of tabaco (Nicotiana tabacum L.) with the Potato virus Y (PVY) were investigated. Obviously, heat stress is associated with the synthesis of Hsp70 protein, which has many important functions alleviating adverse effects of stress conditions (e.g. Hsp70 participates in refolding or degradation of damaged proteins and protein syntetis de novo). The effect of Hsp70 during viral infection is still not fully understood, some studies revealed Hsp70 as a part of viral multiplication and transport processes in plant. In the first experiment performed in this work, higher levels of PVYNTN virus was found in tobacco plants that have been exposed to heat shock after inoculation than in plants only infected. The amount of the virus corresponded with the amount of Hsp70 protein detected immunochemically using a specific antibody. It seems that the plant response to combination of heat stress with viral infection is a...
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The heat-stress-related mortality in the Czech Republic: first results
Kyselý, Jan ; Huth, Radan ; Kříž, B.
The analysis of the HSR mortality in the Czech Republic tries to find answers to the following questions: (i) which treshold value of tempereture (or of another temperature- and humidity- related variable)must be exceeded to observe the HSR mortality, in dependence on time of the year; (ii) which meteorological variable (or an index) accounts for the largest portion of the mortality increase, and what is the best model between meteorological variables and mortality; (iii) what is the dependence of the HSR mortality on the age group and sex; and (iv) whether a synoptic approach based on an objective classification of air masses leads to better results compared to the more traditional methods. The analysis covers the 1990s when some extremely hot summers appeared in the Czech Republic.
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