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Testing of the electrochemical properties of flow-through diamond detector
Pecák, Jakub ; Dejmková, Hana (advisor) ; Vyskočil, Vlastimil (referee)
In the presented bachelor thesis, the functionality of a flow-through multitubular boron- doped diamond electrode (mtBDDE) was verified on two selected neurotransmitters, DA and NE, using the HPLC technique with isocratic elution. An aqueous solution of Britton-Robinson (B-R) buffer was chosen as the mobile phase and a Kinetex EVO C18 reverse column (150 x 4.6 mm) as the stationary phase. The flow rate of the mobile phase was set at 1 ml/min. For electrochemical detection, mtBDDE was used and UV detection at 280 nm was applied as an assistant to the main detector. The electrochemical response of the DA and NE solution (cΘ = 1.10-4 mol/dm3 ) was investigated in the form of hydrodynamic voltammograms over a potential range from 0.1 V to 1.3 V in different pH ranges of the mobile phase (2.5; 4.0; 6.0; 8.0 and 10.0). It was found that the potential wave shifts to lower potentials with increasing pH of the mobile phase, while the response of individual analytes does not change significantly with pH. Under optimal conditions, i.e., mobile phase B-R buffer at pH = 6 and a potential loaded on the mtBDDE of 0.6 V, the repeatability and reproducibility of the measurement results were verified. The electrode gave repeatable results (RSD < 4 %) comparable to the UV detector used (RSD ≤ 4 %) over the series of...
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The influence of Mozart's effect on basketball shooting accuracy in professional basketball players.
Kranjčevič, Stefan ; Pánek, David (advisor) ; Pavlů, Dagmar (referee)
Title: Mozart effect on shooting accuracy of professional basketball players Aims: The aim of this thesis is to demonstrate whether the Mozart effect impacts the success rate of free throws in young premier league basketball players compared to shooting without music. Methods: As this is a pilot study, the data acquisition on this topic first started by examining the previously written text on the Mozart. The project is an experimental pilot study involving 21 probands who were randomized into three groups. The experiment is divided into 2 parts. In the first part participants are asked to shoot one hundred free throws in five blocks of twenty. In the second part, the participants are exposed to different types of acoustic stimulus during which they throw another hundred free throws. All measurements were taken after conditioning or shooting practice for the most authentic replication of the game situation. The process of the practical part was carried out in accordance with the CRISP-DM methodology, which is one of the most widely used methodologies for knowledge capture from databases (KCD). Results: The results show a borderline low correlation between shooting with and without music, according to Pearson's correlation coefficient, with a value of 0.33. The aim of using this statistical function...
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Graphene dopamine biosensor and gate effect
Krajíčková, Kateřina ; Šimšíková, Michaela (referee) ; Bartošík, Miroslav (advisor)
This bachelor’s thesis focuses on investigating the interaction between biochemical substances (dopamine solutions) and graphene, using field effect transistor (FET) based sensors. Graphene possesses unique properties, including biocompatibility, high charge carrier mobility, and surface sensitivity, making it an ideal material for biosensing devices. In these sensors, graphene is employed as the conductive sensing channel within fieldeffect transistors. By utilizing sensors with an FET arrangement, the doping of graphene induced by adsorbed atoms or molecules can be experimentally determined through the observation of the shift in the position of the Dirac point. The measurements can be performed using either the bottom-gated or electrolytic top-gated configuration of the FET sensor, and the thesis explores the differences between the two setups. Furthermore, it investigates the impact of the distance between the graphene and top-gate electrode on the sensor’s response. The results of these measurements are represented by transfer curves, which exhibit characteristic peaks indicating the charge neutrality point, known as the Dirac point, of graphene.
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Optimization of anodic and cathodic pre-treatments of boron doped diamond electrode surfaces for electroanalysis
Baudisová, Kateřina ; Fischer, Jan (advisor) ; Dejmková, Hana (referee)
This bachelor thesis deals with the optimization of anodic and cathodic pretreatment of BDD electrodes and subsequent comprehensive characterization and comparison of electrochemical parameters of unmodified, O-terminated and H-terminated BDD electrodes. The study was carried out using cyclic voltammetry of two model inner sphere redox systems [Fe(CN)6]4−/3− and dopamine/dopamine-o-quinone. Boron concentrations in the BDD films ranged from 500 ppm to 8000 ppm. O-termination of the electrode surface was most effectively achieved when an activation potential of Eakt = +3.0 V was inserted for 10 min into 0.5 mol l−1 H2SO4. For the dopamine/dopamine-o-quinone system, the O-terminated BDD electrodes caused deceleration of electron transfer compared to the unmodified BDD electrodes, which was accompanied by an increase in the potential difference with the highest ΔEp at 500 ppm BBD electrode (ΔEp = 711 mV). To achieve the H-terminal surface, single and cumulative reduction was tested by inserting Eakt = −3.0 V. A sufficient single reduction time for the [Fe(CN)6]4−/3− system was determined to be 60 s, whereas in the case of dopamine, the electrochemical parameters did not stabilize at the selected reduction times, but the cathodic and anodic peak signal at the H-terminated 1000 ppm BDD electrode was up...
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Hormonal synchronization of fetal circadian clocks in suprachiasmatic nuclei
Kapsdorferová, Viktória ; Sumová, Alena (advisor) ; Sehadová, Hana (referee)
Rhythmic non-light maternal signals, such as various behavioral, neurohumoral or metabolic factors, may play a key role in synchronization of the fetal circadian clock. This diploma thesis focuses more closely on the possible role of leptin and dopamine in the hormonal synchronization of the fetal central clock resides in the suprachiasmatic nuclei of the hypothalamus. A transgenic mouse fetus, which has luciferase fused to the clock gene Period 2, was used as an experimental model. This allowed us to monitor the operation of the fetal central clock in real time by recording the bioluminescence of tissue explants. Exogenous administration of dopamine during the decline in bioluminescence at normalized circadian time 15 induces larger phase delays compared to vehicle. Leptin, on the other hand, can participate in the development of spontaneous rhythms by stabilizing the integrity of the circadian rhythm, manifested by slowing down the rhythm dampening. At the level of individual cellular oscillators, significant regional differences in amplitude and period were not observed using bioluminescence microscopy. However, a slight dispersion of cell phases was observed after administration of dopamine during decreasing bioluminescence. In the case of leptin, there was a prolongation of period of the cell...
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Placental transport of dopamine and norepinephrine
Turková, Helena ; Štaud, František (advisor) ; Jirkovský, Eduard (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Helena Turková Supervisor: prof. PharmDr. František Štaud, Ph.D. Title of diploma thesis: Placental transport of dopamine and norepinephrine During the whole course of pregnancy, it is important to maintain proper monoamine homeostasis, namely serotonin (5-HT), norepinephrine (NE), and dopamine (DA), which are crucial for proper placental function and fetal development. Monoamines are important neuromodulators, involved in cell proliferation, and differentiation. and neuronal migration. High fetal monoamine secretion during gestation demands a responsible clearance mechanism, as disruption of their balance may lead to long-lasting changes in brain structure and function, provoking a higher risk of attention deficit hyperactivity disorder (ADHD), autism or depression. However, uptake of NE a DA through the fetoplacental unit has not been fully and in detail described. Therefore, in this diploma thesis, we focus on the uptake of NE and DA through ex vivo isolated vesicles of microvillous (MVM) and basal (BM) membranes from healthy human term placentas. Our results show that NE and DA uptake is mediated via high-affinity and low-capacity serotonin (SERT) and norepinephrine (NET) transporters in...
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Possible association of polymorphisms in COMT gene with mental diseases
TOMANOVÁ, Aneta
This thesis i focused on associacion of polymorphisms in COMT gene with mental diseases, primarily on polymorphism Val158Met. The theoretical part provides definitions of COMT gene and enzyme, catecholamines affected by COMT and definition of the polymorphism Val158Met. Next is listing of mental diseases, description and known connection with researched polymorphism. The practical part contains proceeding of PCR RFLP used to detect the genotype. The results were evaluated and connected to the theoretical part of the thesis.
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The effect of a regular exercise on the health of patients with Parkinson´s disease
DUBNOVÁ, Veronika
The bachelor thesis deals with the influence of regular exercise on the health of patients suffering from Parkinson's disease. This disease still remains an incurable disease that affects the human central nervous system and leads to its irreversible damage. Until now, the treatment of the disease has only made it possible to alleviate individual symptoms in the long term and thus maintain the patient's quality of life. An important part from the early stages of the disease is not only a pharmacological treatment but also a rehabilitation. The theoretical part of this work deals with a summary of information about Parkinson's disease, its origin, clinical picture, diagnosis and treatment options. The practical part of the work was processed by the method of qualitative research, which was done on the basis of an input and output examination, medical history taking, an interview and an evaluation of patients' ability to walk. The obtained data were compiled in the form of case reports. The study group consisted of 3 patients with a diagnosis of Parkinson's disease, who regularly attended group exercises once a week. The first goal was to evaluate the patient's condition during regular group exercise and the condition after 8 weeks without a group exercise. The second goal was to create an exercise unit for patients suffering from Parkinson's disease. Due to the small number of probands studied, it is certainly not possible to state whether the regular exercise once a week was sufficient in the treatment of Parkinson's disease. However, it was obvious that the patients felt good during the exercise, it positively affected their psychosocial condition and also their condition. Although I did not come to clear conclusions, I presume that the chosen therapy was beneficial.
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Placental transport of dopamine and norepinephrine
Turková, Helena ; Štaud, František (advisor) ; Jirkovský, Eduard (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Helena Turková Supervisor: prof. PharmDr. František Štaud, Ph.D. Title of diploma thesis: Placental transport of dopamine and norepinephrine During the whole course of pregnancy, it is important to maintain proper monoamine homeostasis, namely serotonin (5-HT), norepinephrine (NE), and dopamine (DA), which are crucial for proper placental function and fetal development. Monoamines are important neuromodulators, involved in cell proliferation, and differentiation. and neuronal migration. High fetal monoamine secretion during gestation demands a responsible clearance mechanism, as disruption of their balance may lead to long-lasting changes in brain structure and function, provoking a higher risk of attention deficit hyperactivity disorder (ADHD), autism or depression. However, uptake of NE a DA through the fetoplacental unit has not been fully and in detail described. Therefore, in this diploma thesis, we focus on the uptake of NE and DA through ex vivo isolated vesicles of microvillous (MVM) and basal (BM) membranes from healthy human term placentas. Our results show that NE and DA uptake is mediated via high-affinity and low-capacity serotonin (SERT) and norepinephrine (NET) transporters in...
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