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Biodegradation of polychlorinated biphenyls by white - rot fungi and their enzymes
Linhartová, Lucie ; Bořek Dohalská, Lucie (advisor) ; Černá, Věra (referee)
Polychlorinated biphenyls (PCB) represent relevant persistent organopollutants of the environment and the estimated amount of PCB released into the environment is 750000 metric tons. White-rot fungi have been studied for long time due to their degradative potential toward various aromatic pollutants and it is known that these fungi are able to decompose PCB in vivo. Biodegradation of PCB by the fungus Pleurotus ostreatus was studied in the frame of this work. A high degradative efficiency of P. ostreatus was observed in the first set of experiments, even in the presence of relative high amount of added PCB. Fungus was able to transform 780±50 µg out of the intial amount 1000 µg in 20 ml of a cultivation media within 42 days. A decrease in toxicity was recorded during the degradation that suggests the suitability of this organism for a practical use in decontamination. In vitro experiments with purified laccase induced with Cu2+ from this fungus did not prove any participation of the enzyme in the first step of PCB transformation. The enzyme did not show an ability to degrade PCB even after purification from cultivation media containing PCB. It was found that the first step of PCB transformation can be performed by an intracellular process with microsomal fraction. A degradation of 44-67% was observed for...
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Establishment of the method for determination of selected congeners of polychlorinated biphenyls in water using GC-ECD
TALAVÁŇOVÁ, Eliška
This bachelor thesis deals with the determination of selected congeners of polychlorinated biphenyls in water using GC-ECD. Firstly, the development and optimisation of the analytical method was performed. The following parameters of the analytical method were chosen for optimization: carrier gas flow rate, sample volume, injector temperature, splitless time, initial column temperature, temperature gradient and detector temperature. Then, extraction time of the liquid-liquid extraction was optimised. Finally, the method was successfully validated and applied to the analysis of a real tap water sample.
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Rostliny a mikroorganismy zajišťující cyklus PCB a chlorbenzoátů v životním prostředí
Macková, Martina ; Vrchotová, Blanka ; Frančová, Kateřina ; Rezek, Jan ; Beranová, K. ; Najmanová, Jitka ; Lovecká, P. ; Tříska, J. ; Vrchotová, N. ; Uhlík, Ondřej ; Sylvestre, M. ; Demnerová, K. ; Macek, Tomáš
Bacterial metabolism of plant PCB degradation products and degradation of chlorobenzoic acids by plant cells were studied.
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