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Utilization of confocal microscopy to study of degradation of organic dye in cell biology
Trnová, Kateřina ; Provazník, Ivo (referee) ; Čmiel, Vratislav (advisor)
This thesis focuses on the study of the life of a fluorescent dye. It also examines changes in its emission spectrum determined with the aid of development of fluorescence intensity over the long term sensing in several kinds if of cella. The general part of the thesis with the issues of confocal microscopy, fluorescent radiation and maks. Furthermorer, an analysis of methods for measuring fluorescence lifetime is done. In the thesis there is elaborated a description of the program, whitch was designed for the analysis of the collected output of the used confocal microscope. Sebsequently, the results are evaluated.
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Advanced methods for cardiac cells contour detection
Spíchalová, Barbora ; Čmiel, Vratislav (referee) ; Odstrčilík, Jan (advisor)
This thesis focuses on advanced methods of detecting contours of the cardiac cells and measuring their contraction. The theoretical section describes the types of confocal microscopes, which are used for capturing biological samples. The following chapter is devoted to the methods of cardiac cells segmentation, where we are introduced to the generally applied approaches. The most widely spread methods of segmentation are active contours and mathematical morphology, which are the crucial topics of this thesis. Thanks to the those methods we are able in the visual data to accurately detect required elements and measure their surface chnage in time. Acquired theoretical knowledge leads us to the practical realization of the methods in MATLAB.
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System for measurement of concentration of intracellular calcium in LabVIEW
Pochlopeň, Timotej ; Ronzhina, Marina (referee) ; Čmiel, Vratislav (advisor)
Master‘s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. The theory dealing with confocal microscopy is presented and two techniques are compared. Literary solution is presented. The practical part describes the program with a graphical user interface used for acquisition and analysis of data measuring dynamics of intracellular calcium concentration. The experimental part describes the preparation and measurement of cells and evaluation of experiments.
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3D vizualizace velkých biologických vzorků nasnímaných konfokálním mikroskopem
Čapek, Martin ; Janáček, Jiří ; Karen, Petr ; Kubínová, Lucie ; Smrčka, P. ; Hána, K.
Digital volume reconstruction is a technique for rendering and visualization of a biological specimen which is greater than the field of view of a used optical instrument - a confocal laser scanning microscope in our case. Prior to the volume reconstruction, large biological specimens are cut to thin physical slices. The first step of volume reconstruction is acquisition of sets of digital volume images (spatial tiles which overlap) from all studied physical slices. The second step is composition of neighbouring spatial tiles of the same physical slice. The third reconstruction step is registration and merging of digital volumes of neighbouring physical slices of the specimen. The resulting large digital volumes are rendered and visualized using a VolumePro hardware board that offers real-time 3D volume rendering. In this paper we show a reconstruction of a chick embryonic kidney
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