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The effect of acute cold and permanent light to left ventricular of the rat heart
Vítková, Ivana ; Žurmanová, Jitka (advisor) ; Bendová, Zdeňka (referee)
Acute cold exposure increases the risk of sudden cardiac events, similarly exposure to constant light negatively affects the cardiovascular system. However, the individual effects of these factors and the effect of their combination on cardiomyocytes are not yet known. The thesis deals with the influence of a 3 day cold exposure and constant light on the expression of β-adrenergic receptors and associated G-proteins in association with apoptotic signals in the left ventricle of the Wistar rat heart. In this work apoptotic proteins BAX, BCL2, caspase 8 and important components of β-adrenergic signalization - β1, β2, G-proteins, Gas, Gi1/2 and Gi3 were determined. The relative expression of the proteins was analyzed by Western blotting. The results confirm the detrimental effect of cold and light exposure. However, the synergistic effect of these two stressors shows surprising results.
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Energy supply for greenhouse production
Najbrtová, Zuzana ; Poláčik, Ján (referee) ; Pospíšil, Jiří (advisor)
The import of the vegetable has prevailed in the long run over the own production. In connection with this reality and increasing demand for the food with higher quality the production greenhouse is more often built. The providing heat, cold and electricity for the year-round operation is related to the construction and working of these objects. The theoretical part of this theses describes possible ways how to cover energy consumption for the model greenhouse in Kožichovice, Třebíč region. The heat losses and the year-round heat consumption were stated for the selected model and compared with the exact values from Kožichovice. The following method of energy supply was selected based on the calculated energy consumption - the separate cogeneration unit, cooperation of several cogeneration units, a biogas station, a heat pump and central heating system. These methods of energy supply were subjected to technical and economical comparison.
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Cardioprotective effect of adaptations to hypoxia and cold: Connexin 43 and hexokinase 2
Kohútová, Jana
Ventricular arrhythmias are the main cause of death worldwide. An increased incidence of arrhythmias in the heart of mammals is accompanied by a remodeling of the cellular distribution gap between the channels of mainly connexin 43 (Cx43). Recently has been demonstrated significant effect of mitochondria and their association with arrhythmogenesis. Various pathological conditions alter the expression and / or distribution of Cx43, depending on the phosphorylation status. but also on altering the association of hexokinase with mitochondria, which reduces the likelihood of apoptosis activation. Adaptation to intermittent hypobaric hypoxia potentiates endogenous pathways reducing the incidence of ventricular arteries, whereas continuous normobaric hypoxia does not have this effect. Another studied model is cold acclimatization, which has been known for several decades by known effects on human health. However, the heart study of these models in relation to Cx43 is missing. Our goal was to determine the expression of Cx43, phosphorylated Cx43 (p-Cx43) and hexokinase (HK1, HK2) and their distribution in cardiomyocytes. In addition, the expression of Cx43 upstream kinases, protein kinase A, protein kinase G, casein kinase 1 in normoxic and hypoxic left ventricles of rats, along with Cx43 distribution...
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The effect of season on mitochondrial swelling of rat heart after cold acclimation
Kašík, Petr ; Horníková, Daniela (advisor) ; Holzerová, Kristýna (referee)
Risk of death from cardiovascular disease is substantially increased throughout winter season and by exposure of organism to acute cold stress. However, several studies have suggested that some cold acclimation models could act cardioprotectively. The thesis is focused on effects of acute and chronic cold acclimation on swelling of rat heart mitochondria. Mitochondrial swelling is, besides other things, caused by massive influx of Ca2+ into the mitochondria especially during ischemia-reperfusion injury. By that, mitochondria permeability transition pore is being opened and subsequently, this could end up in mitochondrial burst and cell death, eventually. Lower rate of mitochondrial swelling indicates greater mitochondrial resistance. The results express higher mitochondrial resistance after acute cold exposure and chronic cold acclimation. Key words: cold, mitochondria, Ca2+ ions, swelling, MPTP, myocardium, ROS, ischemia
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Relationship between the renin-angiotensin-aldosterone system and the heart and its influence during 5°C cold acclimation.
Hlaváčová, Diana ; Horníková, Daniela (advisor) ; Heleš, Mário (referee)
Heart as one of the life-important organs is influenced by many ongoing processes in organism. Even the smallest molecular changes can importantly influence heart's physiology and activity. Last years, there were many studies concentrating on clarification of renin-angiotensin- aldosteron system (RAAS) and its influence on heart and kidneys. Cardiovascular diseases are usually caused by changes in RAAS and that is the reason why more and more experts are examining the extent to which diseases can be prevented and heart function improved. Ambient temperature is one of the factors that greatly affects the RAAS and the heart. In this thesis I tried to summarize current knowledge about RAAS and its effects on organism, focusing on influencing the physiology of the heart. While ambient temperature has significant effect on the RAAS and the heart, this thesis points to the knowledge associated with cold acclimation at 5 řC. Key words: renin-angiotensin-aldosteron system, heart, cold, hypertension, adrenergic receptors, natriuretic peptides
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Cardioprotective effect of adaptations to hypoxia and cold: Connexin 43 and hexokinase 2
Kohútová, Jana
Ventricular arrhythmias are the main cause of death worldwide. An increased incidence of arrhythmias in the heart of mammals is accompanied by a remodeling of the cellular distribution gap between the channels of mainly connexin 43 (Cx43). Recently has been demonstrated significant effect of mitochondria and their association with arrhythmogenesis. Various pathological conditions alter the expression and / or distribution of Cx43, depending on the phosphorylation status. but also on altering the association of hexokinase with mitochondria, which reduces the likelihood of apoptosis activation. Adaptation to intermittent hypobaric hypoxia potentiates endogenous pathways reducing the incidence of ventricular arteries, whereas continuous normobaric hypoxia does not have this effect. Another studied model is cold acclimatization, which has been known for several decades by known effects on human health. However, the heart study of these models in relation to Cx43 is missing. Our goal was to determine the expression of Cx43, phosphorylated Cx43 (p-Cx43) and hexokinase (HK1, HK2) and their distribution in cardiomyocytes. In addition, the expression of Cx43 upstream kinases, protein kinase A, protein kinase G, casein kinase 1 in normoxic and hypoxic left ventricles of rats, along with Cx43 distribution...
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Cardioprotective effect of adaptations to hypoxia and cold: Connexin 43 and hexokinase 2
Kohútová, Jana ; Žurmanová, Jitka (advisor) ; Radošinská, Jana (referee) ; Barančík, Miroslav (referee)
Ventricular arrhythmias are the main cause of death worldwide. An increased incidence of arrhythmias in the heart of mammals is accompanied by a remodeling of the cellular distribution gap between the channels of mainly connexin 43 (Cx43). Recently has been demonstrated significant effect of mitochondria and their association with arrhythmogenesis. Various pathological conditions alter the expression and / or distribution of Cx43, depending on the phosphorylation status. but also on altering the association of hexokinase with mitochondria, which reduces the likelihood of apoptosis activation. Adaptation to intermittent hypobaric hypoxia potentiates endogenous pathways reducing the incidence of ventricular arteries, whereas continuous normobaric hypoxia does not have this effect. Another studied model is cold acclimatization, which has been known for several decades by known effects on human health. However, the heart study of these models in relation to Cx43 is missing. Our goal was to determine the expression of Cx43, phosphorylated Cx43 (p-Cx43) and hexokinase (HK1, HK2) and their distribution in cardiomyocytes. In addition, the expression of Cx43 upstream kinases, protein kinase A, protein kinase G, casein kinase 1 in normoxic and hypoxic left ventricles of rats, along with Cx43 distribution...
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The effect of acute cold and permanent light to left ventricular of the rat heart
Vítková, Ivana ; Žurmanová, Jitka (advisor) ; Bendová, Zdeňka (referee)
Acute cold exposure increases the risk of sudden cardiac events, similarly exposure to constant light negatively affects the cardiovascular system. However, the individual effects of these factors and the effect of their combination on cardiomyocytes are not yet known. The thesis deals with the influence of a 3 day cold exposure and constant light on the expression of β-adrenergic receptors and associated G-proteins in association with apoptotic signals in the left ventricle of the Wistar rat heart. In this work apoptotic proteins BAX, BCL2, caspase 8 and important components of β-adrenergic signalization - β1, β2, G-proteins, Gas, Gi1/2 and Gi3 were determined. The relative expression of the proteins was analyzed by Western blotting. The results confirm the detrimental effect of cold and light exposure. However, the synergistic effect of these two stressors shows surprising results.
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Increasing the energy efficiency of industrial heating plant
Spišák, Lukáš ; Lisý, Martin (referee) ; Kracík, Petr (advisor)
This bachelor thesis consists of two parts. The introductory chapters are devoted to the basic description of cogeneration and trigeneration technologies, focusing on the description of cogeneration units and cooling devices. The second part of the thesis compares two specific ways of using cogeneration units and gas boilers in the CHEMOSVIT ENERGOCHEM a.s. in the town Svit. Subsequently, both variants are evaluated energetically and economically.
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Physiological mechanisms of abiotic stress tolerance in Sorghum bicolor
Kratochvíl, Jan ; Konrádová, Hana (advisor) ; Lhotáková, Zuzana (referee)
Current agriculture is facing a serious challenge of decreasing precipitation and irregular occurrence of drought periods including their unfavorable distribution during the vegetation season. This leads to growing interest in planting highly drought-resistant crops like sorghum. In comparison with other crops, sorghum excels in low water demand, though exhibits high susceptibility to low temperatures, which hampers its spread to new regions. Surprisingly, there is not enough information about the nature of sorghum's reaction to cold exposure. The aim of this diploma thesis was to describe reactions of young sorghum plants exposed to cold stress, low water availability and their combination and to verify the possibility of plant hardening through previous low-stress load. The special focus was paid to changes in carbohydrate metabolism, which plays generally very important role in plant defense reactions. The other analyzed physiological traits were leaf tissue osmotic potential, proline content and basic morphometric characteristics. Experimental design consisted of pot experiments conducted in growth chambers and the experiments performed under controlled conditions in vitro, using two sorghum genotypes "Ruzrok" and "01Z1800012". Both genotypes exhibited similar response to stress treatment....
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