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The meaning of hydratation old people from the perspective of nurses
GRIMM, Viktor
The aim of the bachelor work is to fulfill the following: 1. to research the nurses´knowledge of seniors´ hydratation, 2. to research the nurses´procedure of monitoring the seniors´hydratation and 3. to research the nurses´role in seniors´care concerning hydratation. To achieve the aims mentioned there have been seven research questions to be answered: What is the nurses´ knowledge in the field of hydratation?, How do nurses keep their knowledge and how do they extend it? Are there any significant differences as for maintaining and upgrading knowledge regarding hydratation among nurses of individual units? What are the hydratation signs nurses monitor and evaluate? Are there any differences as for the seniors´hydration procedures among nurses of individual units? What interventions concerning seniors´ hydratation care do nurses carry out? And How do nurses perceive their part of care connected with seniors´liquid intake? To get valuable information for the empiric part of the work there has been carried out a qualitative research method for which a semi-structured dialogue has been used.This dialogue included 22 open questions which were completed by follow-up questions if necessary, see the attachment 1.The questions were aimed at the following: what do nurses monitor while taking care of seniors in danger of hydratation, what are the key interventions the nurses carry out but also if they are being educated in this field and how important do they consider their part. The respondents were 6 general nurses who provide care for geriatric patients endangered by dehydratation. To analyse the research results the method of open coding, pencil-paper, was used. For better orientation the research results have been put into four main categories and several sub-categories. These logically created categories were set to deal with individual hydratation issues from the nurses´point of view, complexly, taking wider circumstances into consideration including nurses´education and their personal opinions on this topic.Thank to opinions of the general nurses, the most competent ones, the work´s results provide us with a complex view on the matter and that is why it can be used as the source of information not only for students, hospital attendants and nurses but also for common people who look after a senior at home and that is why they are highly interested in this issue.
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Pre-competition reduction of body weight in MMA
Hrdlička, Jaroslav ; Coufalová, Klára (advisor) ; Pavelka, Radim (referee)
Title: Pre-competition reduction of body weight in MMA Objectives: The main aim of this work was to identify what weight reduction methods are used by professional MMA fighters and how many kilograms they reduce before fights. We also wanted to determine the time they start to work with their body weight and whether they feel any psychological changes or fatigue due to weight reduction. We were also interested in what supplements fighters use during weight cut. Methods: A survey method was used for data collection. The research sample consisted of 20 professional MMA fighters between the ages of 18 and 40. Results: We have found out that MMA fighters usually reduce their weight through reduced caloric intake and subsequent dehydration the most. The majority of respondents reported a reduction of 6 to 10 kilograms in the course of 2 to 4 weeks before the fight. Respondents feel weakened during weight loss and experience psychological impacts such as irritation, mood swings, aggression and sedation. Before the fight, 60% of fighters do not experience any signs of weakness, as the time period from weighting to the fight itself is sufficient to allow them to regenerate. The most commonly used supplements during weight loss are BCAAs, fat burners, proteins, multivitamins and multiminerals. Keywords:...
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Characterisation and chemical modification of halloysites
Vašutová, Vlasta ; Hradil, David (advisor) ; Pospíšil, Miroslav (referee)
Twelve halloysites from different sources in Slovakia, Turkey, China, New Zealand and U.S.A. have been characterized by combination of analytical methods together with the commercial sample of halloysite supplied by Sigma-Aldrich. The aim of this work was to select suitable candidates for to be used as carriers of porphyrine photoactive molecules. In nature, the formation of halloysite is related to the weathering of magmatic rocks or to the hydrothermal alteration of volcanic rocks, frequently in the contact with limestones. Halloysite belong to the kaolinite group, but, contrarily to kaolinite, it contains molecules of water in the interlayer space. It occurs in two forms: hydrated halloysite (10 ?) and dehydrated halloysite (7 ?).Dehydrated halloysites contain more admixtures than hydrated ones ? typically kaolinite, quartz, cristobalite, alunite, gibbsite and in one case also potassium mica. In samples containing both dehydrated halloysite and kaolinite their 001 diffractions overlap. Interaction with formamide was used in these cases to increase the interlayer space of halloysite and thus shift its basal diffraction to lower angles. The basal 001 diffraction of kaolinite after this treatment remains on 7 ?. Silver thiourea method (AgTU) was used to measure the cationic exchange capacity (CEC). Silver...
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A new approach to the electroanalysis of primary bile acids and related steroids
Klouda, Jan
In this doctoral thesis, a novel method for the determination of primary bile acids cholic acid and chenodeoxycholic acid is presented. Bile acids play various vital roles in the mammalian body. Moreover, their determination is extremely helpful in liver and biliary disease diagnosis and management. These saturated organic compounds lack strong chromophores and fluorophores in their structure, and thus are usually hard to detect in spectroscopy. For this reason, either instrumentally advanced but expensive methods, such as mass spectrometry, or less reliable enzymatic methods are commonly employed in bile acids quantitation. Hence, the demand for simple and reliable methods for their determination is strong. Bile acids are also known to be virtually inert for direct electrochemical oxidation. Herein, a simple method for their chemical activation for electrochemical oxidation on bare electrode materials was developed, optimized and applied to cholic acid and chenodeoxycholic acid determination. The activation is based on a dehydration reaction of a primary bile acid with 0.1 mol L-1 HClO4 in acetonitrile (water content 0.55%) that introduces double bond(s) into the originally fully saturated steroid core. This naturally increases the electron density in the structure, and thus allows electrochemical...
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A new approach to the electroanalysis of primary bile acids and related steroids
Klouda, Jan
In this doctoral thesis, a novel method for the determination of primary bile acids cholic acid and chenodeoxycholic acid is presented. Bile acids play various vital roles in the mammalian body. Moreover, their determination is extremely helpful in liver and biliary disease diagnosis and management. These saturated organic compounds lack strong chromophores and fluorophores in their structure, and thus are usually hard to detect in spectroscopy. For this reason, either instrumentally advanced but expensive methods, such as mass spectrometry, or less reliable enzymatic methods are commonly employed in bile acids quantitation. Hence, the demand for simple and reliable methods for their determination is strong. Bile acids are also known to be virtually inert for direct electrochemical oxidation. Herein, a simple method for their chemical activation for electrochemical oxidation on bare electrode materials was developed, optimized and applied to cholic acid and chenodeoxycholic acid determination. The activation is based on a dehydration reaction of a primary bile acid with 0.1 mol L-1 HClO4 in acetonitrile (water content 0.55%) that introduces double bond(s) into the originally fully saturated steroid core. This naturally increases the electron density in the structure, and thus allows electrochemical...
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Drinking regime in terms of evidence based medicine
Pihera, Jan ; Procházka, Michal (advisor) ; Vitujová, Marie (referee)
Bachelor thesis "Drinking regime in terms of evidence based medicine" is focused on complex take on the topic of fluid intake based on current knowledge and most recent studies. The content of this work consists of body water function and hydration theoretical summary. Based on this summary is experimental part which should confirm the relationship between hydration and performance.
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Development of voltammetric methods for detection of phytosterols on carbon-based electrodes
Zelenský, Michal ; Schwarzová, Karolina (advisor) ; Fischer, Jan (referee)
The aim of this diploma thesis was to develop an electrochemical method for three phytosterols, namely stigmasterol β-sitosterol and ergosterol using voltammetry techniques on glassy carbon electrode and boron doped diamond electrode. The work builds on previous results, where it was shown that the electrochemical oxidation of phytosterols requires perchloric acid in acetonitrile. When this acid is used, a dehydration reaction occurs. Calibration dependences were measured here for all phytosterols in an environment with a water content of 0.43%. The stability of the signal with increasing distilled water content was further investigated. Then the calibration dependences in the increased water content were measured again. If we want to use HPLC-type separation techniques, we must first find out how the phytosterols will behave in this environment, and therefore the signal stability on the buffer content was investigated. The buffers used were phosphate and acetate buffer. Subsequently, the concentration dependence was measured in an environment with a higher content of phosphate buffer. Furthermore, the possibility of detecting two phytosterols at once was investigated. Key words: dehydration, glassy carbon electrode, boron doped diamond electrode, oxidation, phytosterols, voltammetry
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Development of electroanalytical methods for detection of bile acids possessing 7α hydroxyl group
Jelšíková, Kristýna ; Schwarzová, Karolina (advisor) ; Dejmková, Hana (referee)
This master's thesis contains a study of electrochemical processes of selected bile acids possessing 7 hydroxyl group (cholic, chenodeoxycholic and −muricholic). The measurements were performed on boron−doped diamond electrode in the non-aqueous medium of acetonitrile and perchloric acid (water content 0.55 %) by cyclic voltammetry. It is known that the electrochemical activity of 7 bile acids is increased by a dehydration reaction between perchloric acid and the 7 bile acid. The subject of the study was the stability of the voltammetric response of chemically activated bile acids in the region of negative potentials. It was found that the presence of oxygen in the measured solution is an important factor for obtaining the cathodic signal of 7 bile acids. It probably performs a regenerative function; the product of the electrochemical reduction is re-oxidized in its presence, which leads to an increase in the voltammetric response. At the same time, it is important that the direction of the scan in cyclic voltammetry first proceeds to positive values. A potential of +2.0 V (vs. Ag/AgNO3 in acetonitrile) must be reached for the HO● radicals to be formed. It is these radicals that presumably lead to the formation of the product(s) of bile acids electrochemical oxidation that can be subsequently...
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A new approach to the electroanalysis of primary bile acids and related steroids
Klouda, Jan ; Schwarzová, Karolina (advisor) ; Skopalová, Jana (referee) ; Vyskočil, Vlastimil (referee)
In this doctoral thesis, a novel method for the determination of primary bile acids cholic acid and chenodeoxycholic acid is presented. Bile acids play various vital roles in the mammalian body. Moreover, their determination is extremely helpful in liver and biliary disease diagnosis and management. These saturated organic compounds lack strong chromophores and fluorophores in their structure, and thus are usually hard to detect in spectroscopy. For this reason, either instrumentally advanced but expensive methods, such as mass spectrometry, or less reliable enzymatic methods are commonly employed in bile acids quantitation. Hence, the demand for simple and reliable methods for their determination is strong. Bile acids are also known to be virtually inert for direct electrochemical oxidation. Herein, a simple method for their chemical activation for electrochemical oxidation on bare electrode materials was developed, optimized and applied to cholic acid and chenodeoxycholic acid determination. The activation is based on a dehydration reaction of a primary bile acid with 0.1 mol L-1 HClO4 in acetonitrile (water content 0.55%) that introduces double bond(s) into the originally fully saturated steroid core. This naturally increases the electron density in the structure, and thus allows electrochemical...
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Aplikace kryoterapie při ozdravování regenerantů Prunus persica (L.) od rostlinných virů
Fronková, Hana
Final thesis was worked out on theme Aplication of cryotherapy as a tool for plant viruses eradication from Prunus persica (L.) Batsch regenerants. Experiment was conducted on Faculty of Agronomy of the Mendel University in Brno and in the Crop Research Institute in Prague-Ruzyně. Literary research describes information about peach cultivation in vitro and plant pathogen eradication by cryotherapy. These information has been used in practical part of the final thesis. Plant material of the ´Redhaven´variety of peach was used for virus eradication by cryotherapy. Cryotherapy was applied on shoot tips. In the end of final thesis shoot tips viability and regeneration were evaluated.
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