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Nanostructured gold electrodes for determination of glucose in blood
Farka, Z. ; Juřík, T. ; Kovář, D. ; Podešva, Pavel ; Foret, František ; Skladal, P.
Fast and sensitive devices for monitoring of glucose are crucial in today’s medicine. Most of the commercial glucometers are currently based on enzymatic catalysis which provides reasonable sensitivity but has a drawback in limited enzyme stability. With the development of novel surfaces, new generation of non-enzymatic sensors characterized by high sensitivity and stability is emerging. In this work, gelatin-templated nanostructured gold electrodes were developed and applied for non-enzymatic glucose determination. The analysis was done by direct electrochemical oxidation during cyclic voltammetry and amperometry with the limit of detection at 1.3 μM. The ability to detect physiological levels glucose in presence of interferents was demonstrated on deproteinized human blood serum.
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Quantum dot-based immunoprobe for optical and electrochemical detection
Dvořáková, V. ; Čadková, M. ; Datinská, Vladimíra ; Chałupniak, A. ; Korecká, L. ; Merkoci, A. ; Klepárník, Karel ; Foret, František ; Bílková, Z.
Three-stage labeling strategy allows the preparation of specific conjugates composed of required antibodies and quantum dots as an extremely sensitive bio-probe applicable in varied immunoassays with following optical and electrochemical detection. This approach is essentially based on carbodiimide chemistry however each single step of whole procedure is separated and controlled which makes this applied protocol oriented, highly efficient and it enables gain of pure antibody-conjugated quantum dots in ready-to-use condition.
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Comparison of ionisation properties of aetma-labeled saccharides with common labels
Partyka, Jan ; Foret, František
We have tested (2-aminoethyl)trimethylammonium (AETMA) as a label for analysis of oligosaccharides by capillary electrophoresis with electrospray (ESI) mass spectrometry detection and compared its performance to taurine, 2-aminobenzoic acid, 2-aminobenzamide and 8-aminopyrene-1,3,6-trisulfonic acid. The AETMA-labeled saccharides were provided higher ionization signals in positive mode than native sodium or ammonium adducts and equal or higher signals than saccharides labeled by the more commonly used labels tested in this study.
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