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Sprayable elastic biopolymer hydrogels for wound healing
Waclawiková, Petra ; Nedomová, Eva (oponent) ; Vojtová, Lucy (vedoucí práce)
The main aim of the presented bachelor thesis is focused on the preparation and characterization of sprayable hydrogels from suitable non-toxic substances, in order to develop gels applicable in wound healing management. The theoretical part deals with general properties of hydrogels, methods of their crosslinking and presents selected natural and synthetic materials used for the preparation of hydrogels. It also contains a brief overview of already prepared sprayable hydrogels, which are not yet commercially used. The experimental part describes the selection and use of readily available non-toxic biomaterials and the measurement of the physico-chemical properties of the prepared gels. Gum Karaya, poly(vinyl alcohol) and sodium alginate were selected as the structural polymers together with different physical crosslinkers and additives. The polymers and additives were subjected to solubility test followed by mixing in certain ratios and crosslinked to form preferably transparent hydrogels. Subsequently their optical and rheological properties were valuated. The measurement of optical properties shows that probably gum Karaya affected increasing in both yellowness as well as transparency. By rheological measuring it was demonstrated, that the higher content of gum Karaya reduces the mechanical resistance of the prepared hydrogels. Hydrogel strength reflects both low and high amount of crosslinker where the optimal amount gave the best result. Therefore, the correct ratio of the used substances is the main parameter for obtaining the most effective hydrogel properties suitable for sprayable hydrogel dressings.
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Blue-light crosslinked hydrogels from modified natural gum for medical applications
Waclawiková, Petra ; Michlovská, Lenka (oponent) ; Vojtová, Lucy (vedoucí práce)
The main aims of the presented master’ thesis are focused on the modification of natural gum and its consequential crosslinking via blue or ultraviolet light, in order to develop a sprayable hydrogel applicable for controlled wound healing. The theoretical part of the work deals with the preparation of hydrogels, especially by photopolymerization, and the possibilities of using different light sources for crosslinking. Related to this, the work contains and overview of photoinitiators suitable for the preparation of water-based hydrogels. Thesis introduce examples of functionalization in order to implement a photosensitive functional group into the polymer structure, especially methacrylation. The experimental part of the presented work deals with the preparation and characterization of hydrogels composed of synthetic and natural polymers. The default material was the natural polysaccharide Gum Karaya (GK), which was chemically modified and consequently crosslinked via water-soluble photoinitiator. Chemical modification of Karaya gum saccharide was characterized by two spectroscopic techniques. The attenuated total reflection method of Fourier transform infrared spectroscopy (ATR-FTIR) was used to determine changes in chemical structure. Furthermore, Raman spectroscopy was used as a supplement to the ATR-FTIR method. UV-Vis spectrophotometry was used to evaluate the optical properties of the samples before and after photocrosslinking. The application properties of the synthesized polysaccharide and the prepared hydrogels were tested by the swelling method and the sprayability was verified by rheology. Based on the results, the chemical modification of gum Karaya was successful and it was possible prepare a photocrosslinked hydrogels after the photoinitiator addition.
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Sprayable elastic biopolymer hydrogels for wound healing
Waclawiková, Petra ; Nedomová, Eva (oponent) ; Vojtová, Lucy (vedoucí práce)
The main aim of the presented bachelor thesis is focused on the preparation and characterization of sprayable hydrogels from suitable non-toxic substances, in order to develop gels applicable in wound healing management. The theoretical part deals with general properties of hydrogels, methods of their crosslinking and presents selected natural and synthetic materials used for the preparation of hydrogels. It also contains a brief overview of already prepared sprayable hydrogels, which are not yet commercially used. The experimental part describes the selection and use of readily available non-toxic biomaterials and the measurement of the physico-chemical properties of the prepared gels. Gum Karaya, poly(vinyl alcohol) and sodium alginate were selected as the structural polymers together with different physical crosslinkers and additives. The polymers and additives were subjected to solubility test followed by mixing in certain ratios and crosslinked to form preferably transparent hydrogels. Subsequently their optical and rheological properties were valuated. The measurement of optical properties shows that probably gum Karaya affected increasing in both yellowness as well as transparency. By rheological measuring it was demonstrated, that the higher content of gum Karaya reduces the mechanical resistance of the prepared hydrogels. Hydrogel strength reflects both low and high amount of crosslinker where the optimal amount gave the best result. Therefore, the correct ratio of the used substances is the main parameter for obtaining the most effective hydrogel properties suitable for sprayable hydrogel dressings.
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