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Sprayable elastic biopolymer hydrogels for wound healing
Waclawiková, Petra ; Nedomová, Eva (oponent) ; Vojtová, Lucy (vedoucí práce)
The main aim of the presented bachelor thesis is focused on the preparation and characterization of sprayable hydrogels from suitable non-toxic substances, in order to develop gels applicable in wound healing management. The theoretical part deals with general properties of hydrogels, methods of their crosslinking and presents selected natural and synthetic materials used for the preparation of hydrogels. It also contains a brief overview of already prepared sprayable hydrogels, which are not yet commercially used. The experimental part describes the selection and use of readily available non-toxic biomaterials and the measurement of the physico-chemical properties of the prepared gels. Gum Karaya, poly(vinyl alcohol) and sodium alginate were selected as the structural polymers together with different physical crosslinkers and additives. The polymers and additives were subjected to solubility test followed by mixing in certain ratios and crosslinked to form preferably transparent hydrogels. Subsequently their optical and rheological properties were valuated. The measurement of optical properties shows that probably gum Karaya affected increasing in both yellowness as well as transparency. By rheological measuring it was demonstrated, that the higher content of gum Karaya reduces the mechanical resistance of the prepared hydrogels. Hydrogel strength reflects both low and high amount of crosslinker where the optimal amount gave the best result. Therefore, the correct ratio of the used substances is the main parameter for obtaining the most effective hydrogel properties suitable for sprayable hydrogel dressings.
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Novel hydrogels based on polysaccharides for soft tissue regeneration: preparation and characterization
Nedomová, Eva ; Abdellatif, Abdelmohsan (oponent) ; Vojtová, Lucy (vedoucí práce)
Presented diploma thesis deals with preparation, crosslinking and physico-chemical characterization of natural polysaccharide-based hydrogels. The aim of the work was to evolve elastic thin films with potential application for moist wound healing. The theoretical part summarizes the state-of-art about regeneration of soft tissues and their substitutes (synthetic or nature). There are pointed out the basic information about natural polysaccharide gums (chemical structure, solubility, heat and pH stability etc.), its modification and chemical crosslinking. The experimental part is focused on the modification of natural gum Karaya in order to make transparent hydrogels with adjustable hydrolytical stability. Samples were analyzed by FTIR, TGA followed by evaluation of swelling properties and hydrolytical degradation. Based on the results, chemical modification helped to stabilize polysaccharide hydrogels in water up to 25 days which is useful mainly for moist wound healing (e.g. after burns) because of high values of water uptake (from 85 up to 96%).
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Sprayable elastic biopolymer hydrogels for wound healing
Waclawiková, Petra ; Nedomová, Eva (oponent) ; Vojtová, Lucy (vedoucí práce)
The main aim of the presented bachelor thesis is focused on the preparation and characterization of sprayable hydrogels from suitable non-toxic substances, in order to develop gels applicable in wound healing management. The theoretical part deals with general properties of hydrogels, methods of their crosslinking and presents selected natural and synthetic materials used for the preparation of hydrogels. It also contains a brief overview of already prepared sprayable hydrogels, which are not yet commercially used. The experimental part describes the selection and use of readily available non-toxic biomaterials and the measurement of the physico-chemical properties of the prepared gels. Gum Karaya, poly(vinyl alcohol) and sodium alginate were selected as the structural polymers together with different physical crosslinkers and additives. The polymers and additives were subjected to solubility test followed by mixing in certain ratios and crosslinked to form preferably transparent hydrogels. Subsequently their optical and rheological properties were valuated. The measurement of optical properties shows that probably gum Karaya affected increasing in both yellowness as well as transparency. By rheological measuring it was demonstrated, that the higher content of gum Karaya reduces the mechanical resistance of the prepared hydrogels. Hydrogel strength reflects both low and high amount of crosslinker where the optimal amount gave the best result. Therefore, the correct ratio of the used substances is the main parameter for obtaining the most effective hydrogel properties suitable for sprayable hydrogel dressings.
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Novel hydrogels based on polysaccharides for soft tissue regeneration: preparation and characterization
Nedomová, Eva ; Abdellatif, Abdelmohsan (oponent) ; Vojtová, Lucy (vedoucí práce)
Presented diploma thesis deals with preparation, crosslinking and physico-chemical characterization of natural polysaccharide-based hydrogels. The aim of the work was to evolve elastic thin films with potential application for moist wound healing. The theoretical part summarizes the state-of-art about regeneration of soft tissues and their substitutes (synthetic or nature). There are pointed out the basic information about natural polysaccharide gums (chemical structure, solubility, heat and pH stability etc.), its modification and chemical crosslinking. The experimental part is focused on the modification of natural gum Karaya in order to make transparent hydrogels with adjustable hydrolytical stability. Samples were analyzed by FTIR, TGA followed by evaluation of swelling properties and hydrolytical degradation. Based on the results, chemical modification helped to stabilize polysaccharide hydrogels in water up to 25 days which is useful mainly for moist wound healing (e.g. after burns) because of high values of water uptake (from 85 up to 96%).
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