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Expression and purification of adhesive recombinant proteins, sericin 2 and salivary gland secretion 3
VU, Trang Thanh
Recombinant proteins derived from Bombyx mori Ser-2 gene and Drosophila melanogaster Sgs3 gene were expressed in bacterial expression systems, purified and partially characterised. The identity of the expressed recombinant proteins was verified by mass spectroscopy. The recombinant proteins were tested for their ability to coat hydrophobic surfaces and sequentially serve as a substrate for the attachment of cells in tissue culture. The quality of the recombinant protein surface coating was examined by scanning electron microscopy. The results show that further optimisation for the purification, solubilization and refolding of these recombinant proteins is needed to incorporate their potential as biomaterials in the future.
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Polydopamine coated capillaries for CE separations
Partyka, Jan ; Foret, František ; Vu, T. T. ; Sudor, J.
We have tested CE separation of selected samples in capillaries with polydopamine modified surface. The capillaries were modified by polydopamine or polydopamine with an additive. The polydopamine coating with additive represents a simple and effective procedure for capillary alteration by another modificator such as hydroxyethyl cellulose, hydroxypropyl cellulose etc. In this work, we represent separation data from PrinCE system with UV detection for peptides, proteins and oligosaccharides labeled by 2-aminobenzoic acid.
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