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Molecular Phylogeny of Selected Protists
Kostka, Martin ; Flegr, Jaroslav (advisor) ; Oborník, Miroslav (referee) ; Simpson, Alastair (referee)
Results 1. We sequencedSSU rDNA of Protoopalinaintestinalis, representativeof opalinids,andKarotomorpha,a proteromonaďdwith hithertono moleculardataavailable. Analyses of thesedataconfirmedcloseaffrnityof thetwo families andalsotheparaphylyof proteromonadids- the resultingtopologywas(Proteromonas+(Karotomopha+ Protoopalina)).Slopalinidsbelongedwithin stramenopilesin our analyses. 2. In ouranalyses,thegenusBlastocystis,geneticallyquite variable,formeda sistergroupof slopalinidswithin thegroupof Stramenopila. 3. We sequencesSSU rDNA of two Chilomastixisolates.The sequencesdifferedsubstantiallyin lengthandcomposition,but formeda monophylumin resultingphylogenetictrees. Surprisingly,Retortamona,swÍlsnotreconstructedasa sister groupof Chilomasfrx,butthetwo generaformedaparaphyletic groupfrom which thediplomonadsevolved(withRetortamonas closerto diplomonads).This resultimpliesthattheancestorof diplomonads(includingenteromonads)wasretortamonadidJike. 4. We sequencedandanďyses SSU rDNA of two morphologically well definedamoebae,Mayorella gemmiferaand Saccamoeba limax.We havefoundthatanothersequenceascribedto S.limm andusedin someanalysesprobablyoriginatesfrom a misidontifi€d organism. 5. WohaveprogrammedtheprogramSlowFaster.Itis aunique user-frierrdlytoolleadingauserstep-by-steptbrougbthewhole processof...