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Fermentation of different cereals by the probiotic bacteria Lactobacillus plantarum 299v
Hamalová, Sabina ; Španová, Alena (oponent) ; Prof.Siv Ahrne (vedoucí práce)
The number of humans suffering from various infectious, inflammatory and allergic diseases as well as the occurence of lactose intolerance and high blood cholesterol levels has an increasing tendency. Some of those health disorders are caused by a disbalanced intestinal microbial flora. Probiotics are though to contribute to intestinal microbial balance (Parker, 1974) between the beneficial and adverse bacteria. Therefore, the therapy based on administrating probiotics to humans has attracted research interest. Depending on what desired effect-health disorder should be aimed to leads to the choice of an appropriate probiotic bacterium. Lactobacillus plantarum 299v has proved its beneficial effects on a man and also has met the safety requirements to be recognized as a probiotic bacteria (Probi AB, Sweden). Therefore Lactobacillus plantarum 299v is sold on a marketplace as probiotics in many functional foods, probiotic drink ProViva is one example of them. The aim of this work was to study the fermentation process in oats, barley and soya-based gruels by the strain Lactobacillus plantarum 299v with the focus on the soya and barley substrates. The objectives were to investigate the growth and metabolic activity of Lactobacillus plantarum 299v in association with cereal substrates and further in the mixture of the fermented cereal component with commercial fruit juices. To optimise the fermentation process, several aspects have to be considered. The major role in the designing of the novel fermented food product belongs to the processing and composition of the raw material, the growth capacity and productivity of the bacterial culture and the stability of the final product during storage (De Vuyst, 2000). These parametres are important from the producers point of view. Apart from that, there are also consumers whose product acceptance is based mainly on the sensory characteristics of the final probiotic product, i.e. aroma and taste. The presence and accessibility of different nutrients, which were present in the fermentation media as an outcome of different cereals used, probably resulted in variations of the metabolic pathways which in turn might lead to the distinct differences in the organoleptic properties of the final product.
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Fermentation of different cereals by the probiotic bacteria Lactobacillus plantarum 299v
Hamalová, Sabina ; Španová, Alena (oponent) ; Prof.Siv Ahrne (vedoucí práce)
The number of humans suffering from various infectious, inflammatory and allergic diseases as well as the occurence of lactose intolerance and high blood cholesterol levels has an increasing tendency. Some of those health disorders are caused by a disbalanced intestinal microbial flora. Probiotics are though to contribute to intestinal microbial balance (Parker, 1974) between the beneficial and adverse bacteria. Therefore, the therapy based on administrating probiotics to humans has attracted research interest. Depending on what desired effect-health disorder should be aimed to leads to the choice of an appropriate probiotic bacterium. Lactobacillus plantarum 299v has proved its beneficial effects on a man and also has met the safety requirements to be recognized as a probiotic bacteria (Probi AB, Sweden). Therefore Lactobacillus plantarum 299v is sold on a marketplace as probiotics in many functional foods, probiotic drink ProViva is one example of them. The aim of this work was to study the fermentation process in oats, barley and soya-based gruels by the strain Lactobacillus plantarum 299v with the focus on the soya and barley substrates. The objectives were to investigate the growth and metabolic activity of Lactobacillus plantarum 299v in association with cereal substrates and further in the mixture of the fermented cereal component with commercial fruit juices. To optimise the fermentation process, several aspects have to be considered. The major role in the designing of the novel fermented food product belongs to the processing and composition of the raw material, the growth capacity and productivity of the bacterial culture and the stability of the final product during storage (De Vuyst, 2000). These parametres are important from the producers point of view. Apart from that, there are also consumers whose product acceptance is based mainly on the sensory characteristics of the final probiotic product, i.e. aroma and taste. The presence and accessibility of different nutrients, which were present in the fermentation media as an outcome of different cereals used, probably resulted in variations of the metabolic pathways which in turn might lead to the distinct differences in the organoleptic properties of the final product.
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