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Peptidázy motolic
Kašný, Martin ; Mikeš, Libor (vedoucí práce) ; Kopáček, Petr (oponent) ; Haas, Wilfried (oponent)
90 3. SUMMARY The text above refers about the majority of characterized trematode peptidases; the fundamental enzymes for trematode existence, which are integrated in many physiological processes like pathogenesis, tissue invasion/migration, nutrition, immune evasion and host-parasite interactions. In the history (until 1996), the peptidase catalytic activities in trematode extracts have been monitored. During 1980s and 1990s, the information of first cloned trematode peptidase genes were published and during last three decades cca 90 trematode peptidase sequences belonging to 19 peptidase families of 5 clans have been identified. The most studied trematode peptidases have been of Schistosoma mansoni origin: the serine peptidase - cercarial elastase (of cercariae), cysteine peptidases - cathepsins B, L, F, C plus the asparaginyl endopeptidase SmAE and the aspartic peptidase - cathepsin D (of adult worms and some other life stages). The recent computational cluster analysis revealed that the sequence S. mansoni elastase (the main cercarial penetration enzyme) is quite divergent from other serine peptidases of the S1 family. Cercarial elastase gene was proved in S. mansoni, S. haematobium and Schistosomatium douthitti, but not in the related S. japonicum. Mass spectrometry analysis confirmed cercarial...
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BIRD SCHISTOSOMES: development of schistosomula with focus on Trichobilharzia spp
Chanová, Marta ; Horák, Petr (vedoucí práce) ; Špakulová, Marta (oponent) ; Haas, Wilfried (oponent)
{ ) I SEZNAMVLASTNiCHPUBLIKACi: cHANovA M, vuoNc s, HOMK P- (2007): Trichobilhunia szidatii the rung phase of migration within avian and mmmaliin hosts.Pamitol Res. 100(6): 1243- 1247(IFt.s) cHANovA M.' HoRAKP. (2007): Terminal phase ofbird schistosomiasiscaused by Tichobilhunia regezri (schistosomatidae) in ducks (Anu pktltrhynclros f. domstie). Folia pd6itol 54(2): 105 - 1070F r.o) cHANovA M, BULANToVAJ, MASLo p, HoRiK p (2009): In vito cultivation of erly shistosomula of nasal and visceral bird *histosomes (Trichobilhuziu spp., Schistommatidae). pmsitol Res 104(6):l44s - 14520F 1.5) zAviR V1/sledky pr6ce rozSiiuji dosavadni poaatky o biologii schistosomul ptaiich schistosom. Byly zji5tdni detailni infomace o rlivoji dvou modeloqich druhri rodu Trichobilhania repreantujicich viscer6lni a nm6lni schistosomy. Nejvi;amdjii vlsledky pr6ce: In vilro kultiryaee ' Schistosomuly trmsfomovmd in vivo \ziskmd z tkrini ptadiho hostitele) pieZivaji a dospivaji v podmir/rlieh in vitro v kultivadnim m6diu (RpMI 1620) s ptadimi erytrocyty pfi 37"C a 5o/oCO2. . RPMI 1620 medim se neosvEddilo pro in vitro tramfomrci a n6sledn51.vlvoj ptadich schistosom_ SCM 169, m6dim pro kultivaci lidsklch schistoso4 doplndni kachnimi erytrocl,ty a piisluinj'mi mtimykotiky a antibiotiky,je pro vjvoj rilich schistosomul vhodnd. ' Vlivojovd...
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BIRD SCHISTOSOMES: development of schistosomula with focus on Trichobilharzia spp
Chanová, Marta ; Horák, Petr (vedoucí práce) ; Špakulová, Marta (oponent) ; Haas, Wilfried (oponent)
{ ) I SEZNAMVLASTNiCHPUBLIKACi: cHANovA M, vuoNc s, HOMK P- (2007): Trichobilhunia szidatii the rung phase of migration within avian and mmmaliin hosts.Pamitol Res. 100(6): 1243- 1247(IFt.s) cHANovA M.' HoRAKP. (2007): Terminal phase ofbird schistosomiasiscaused by Tichobilhunia regezri (schistosomatidae) in ducks (Anu pktltrhynclros f. domstie). Folia pd6itol 54(2): 105 - 1070F r.o) cHANovA M, BULANToVAJ, MASLo p, HoRiK p (2009): In vito cultivation of erly shistosomula of nasal and visceral bird *histosomes (Trichobilhuziu spp., Schistommatidae). pmsitol Res 104(6):l44s - 14520F 1.5) zAviR V1/sledky pr6ce rozSiiuji dosavadni poaatky o biologii schistosomul ptaiich schistosom. Byly zji5tdni detailni infomace o rlivoji dvou modeloqich druhri rodu Trichobilhania repreantujicich viscer6lni a nm6lni schistosomy. Nejvi;amdjii vlsledky pr6ce: In vilro kultiryaee ' Schistosomuly trmsfomovmd in vivo \ziskmd z tkrini ptadiho hostitele) pieZivaji a dospivaji v podmir/rlieh in vitro v kultivadnim m6diu (RpMI 1620) s ptadimi erytrocyty pfi 37"C a 5o/oCO2. . RPMI 1620 medim se neosvEddilo pro in vitro tramfomrci a n6sledn51.vlvoj ptadich schistosom_ SCM 169, m6dim pro kultivaci lidsklch schistoso4 doplndni kachnimi erytrocl,ty a piisluinj'mi mtimykotiky a antibiotiky,je pro vjvoj rilich schistosomul vhodnd. ' Vlivojovd...
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Peptidázy motolic
Kašný, Martin ; Mikeš, Libor (vedoucí práce) ; Kopáček, Petr (oponent) ; Haas, Wilfried (oponent)
90 3. SUMMARY The text above refers about the majority of characterized trematode peptidases; the fundamental enzymes for trematode existence, which are integrated in many physiological processes like pathogenesis, tissue invasion/migration, nutrition, immune evasion and host-parasite interactions. In the history (until 1996), the peptidase catalytic activities in trematode extracts have been monitored. During 1980s and 1990s, the information of first cloned trematode peptidase genes were published and during last three decades cca 90 trematode peptidase sequences belonging to 19 peptidase families of 5 clans have been identified. The most studied trematode peptidases have been of Schistosoma mansoni origin: the serine peptidase - cercarial elastase (of cercariae), cysteine peptidases - cathepsins B, L, F, C plus the asparaginyl endopeptidase SmAE and the aspartic peptidase - cathepsin D (of adult worms and some other life stages). The recent computational cluster analysis revealed that the sequence S. mansoni elastase (the main cercarial penetration enzyme) is quite divergent from other serine peptidases of the S1 family. Cercarial elastase gene was proved in S. mansoni, S. haematobium and Schistosomatium douthitti, but not in the related S. japonicum. Mass spectrometry analysis confirmed cercarial...
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