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Production of extracellular polysaccharides by extremophilic prokaryotes
Bystrická, Klaudia ; Smilek, Jiří (oponent) ; Obruča, Stanislav (vedoucí práce)
The presented master's thesis focuses on studying the biotechnological production of extracellular polysaccharides (EPS) by selected thermophilic and halophilic microorganisms. Following the primary screening of selected cultures, the experimental part of this work further investigated the halophilic archaeon Haloferax mediterranei. The aim of this study was to produce and characterize polymers and assess the biotechnological potential of the extremophilic cultures used as EPS producers. During the cultivation of H. mediterranei, three fundamental media were employed, which were subsequently optimized for EPS production. The obtained precipitates underwent analysis using GPC, FTIR, and UHPLC-UV-ESI-MS/MS, allowing for a closer characterization of the acquired product. These experiments demonstrated that with increasing NaCl concentration in the medium, the yield of polysaccharides decreases, and high concentrations of MgSO4 and KCl in the medium did not promote their production as well. The predominant component of the polysaccharide produced by H. mediterranei is mannose, although depending on the medium, it may also contain glucose, galactose, ribose, or N-acetylglucosamine.
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Biotechnological conversion of spent brewery grains into microbial polymers
Bystrická, Klaudia ; Nováčková, Ivana (oponent) ; Obruča, Stanislav (vedoucí práce)
The presented bachelor thesis deals with the study of biotechnological production of polyhydroxyalkoanates (PHA), using hydrolysates from brewer’s spelt grain (BSG) and selected bacterial strains Schlegelella, Halomonas, Tepidimonas and Burkholderia. The thesis aims to determine whether it is possible to use BSG hydrolysates as a substrate for PHA production, with the emphasis on different pretreatment methods of the lignocellulosic material. BSG was submitted to quantitative analysis and the optimum temperature and pH of the commercial enzymatic cocktail used for the hydrolysation was determined. The most effective transformation of sugars to PHA was accomplished by bacteria H. organivorans with the use of a hydrolysate from plasma pretreated BSG. Hydrolysates which were prepared with moderate methods, contained less bacterial inhibitors and sufficient amounts of sugars. These hydrolysates served therefore as preferable substrates for the microorganism growth.
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Biotechnological conversion of spent brewery grains into microbial polymers
Bystrická, Klaudia ; Nováčková, Ivana (oponent) ; Obruča, Stanislav (vedoucí práce)
The presented bachelor thesis deals with the study of biotechnological production of polyhydroxyalkoanates (PHA), using hydrolysates from brewer’s spelt grain (BSG) and selected bacterial strains Schlegelella, Halomonas, Tepidimonas and Burkholderia. The thesis aims to determine whether it is possible to use BSG hydrolysates as a substrate for PHA production, with the emphasis on different pretreatment methods of the lignocellulosic material. BSG was submitted to quantitative analysis and the optimum temperature and pH of the commercial enzymatic cocktail used for the hydrolysation was determined. The most effective transformation of sugars to PHA was accomplished by bacteria H. organivorans with the use of a hydrolysate from plasma pretreated BSG. Hydrolysates which were prepared with moderate methods, contained less bacterial inhibitors and sufficient amounts of sugars. These hydrolysates served therefore as preferable substrates for the microorganism growth.
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