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Possible changes of endoglin expression in endothelial cells.
Tripská, Katarína ; Nachtigal, Petr (advisor) ; Mladěnka, Přemysl (referee) ; Vohnout, Branislav (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Doctoral Degree Program: Pharmacology and Toxicology Candidate: Mgr. Katarína Tripská Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title of Doctoral Thesis: Possible changes of endoglin expression in endothelial cells Endoglin (Eng) is a co-receptor for ligands of the Transforming Growth Factor β superfamily. Multiple studies have demonstrated that Eng is associated with metabolic syndrome-related pathological conditions, such as hypercholesterolemia, hyperglycemia, hypertension, or obesity. However, the exact role of Eng in these pathologies is still unknown. Therefore, the main purpose of this thesis was to elucidate and summarize the role of membrane and soluble Eng in the selected pathologies associated with metabolic syndrome. We demonstrated that soluble Eng (sEng) levels, together with plasma lipids and markers of inflammation, were reduced after every lipoprotein apheresis in patients with familial hypercholesterolemia. This result, in combination with knowledge acquired by the review article, indicates that increased sEng levels are associated with cardiometabolic disorders. Regarding the role of membrane Eng, we showed that blockage of Eng expression by TRC105 results in a decrease...
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Possible changes of endoglin expression in endothelial cells.
Tripská, Katarína ; Nachtigal, Petr (advisor) ; Mladěnka, Přemysl (referee) ; Vohnout, Branislav (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Doctoral Degree Program: Pharmacology and Toxicology Candidate: Mgr. Katarína Tripská Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title of Doctoral Thesis: Possible changes of endoglin expression in endothelial cells Endoglin (Eng) is a co-receptor for ligands of the Transforming Growth Factor β superfamily. Multiple studies have demonstrated that Eng is associated with metabolic syndrome-related pathological conditions, such as hypercholesterolemia, hyperglycemia, hypertension, or obesity. However, the exact role of Eng in these pathologies is still unknown. Therefore, the main purpose of this thesis was to elucidate and summarize the role of membrane and soluble Eng in the selected pathologies associated with metabolic syndrome. We demonstrated that soluble Eng (sEng) levels, together with plasma lipids and markers of inflammation, were reduced after every lipoprotein apheresis in patients with familial hypercholesterolemia. This result, in combination with knowledge acquired by the review article, indicates that increased sEng levels are associated with cardiometabolic disorders. Regarding the role of membrane Eng, we showed that blockage of Eng expression by TRC105 results in a decrease...
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Possibilities of influence of cardiovascular risk markers with a diet enriched with amaranth flour
Chmelík, Zdeněk ; Vrablík, Michal (advisor) ; Piťha, Jan (referee) ; Vohnout, Branislav (referee)
Univerzita Karlova v Praze 1. lékařská fakulta Abstrakt dizertační práce Možnosti ovlivnění markerů kardiovaskulárního rizika stravou obohacenou amarantovou moukou PharmDr. Zdeněk Chmelík Praha 2022 Abstract (EN) Introduction: Diet suitable for lipid metabolism disorders should not increase the total and LDL cholesterol, and ideally should assist in its lowering. Analysis of literary sources suggest that amaranth can meet these properties. Aim: The execution of literary investigation of the issue and experimental verification of lipid- lowering effect of amaranth flour in laboratory conditions. Methods: 50 (Experiment 1) or 40 (Experiment 2) The mice were divided into five (Experiment 1) or four (Experiment 2) groups fed by control mixture (C), high-energy diet (HED), high- energy diet with amaranth (HED+A) and in Experiment 1, by high-energy diet with amaranth flour and simvastatin (HED+A+S) or only with simvastatin (HED+S), and in Experiment 2 a by high-energy diet with squalene (HED+S). Weight gains were measured and TC, LDL and HDL cholesterol determined. Results: Feeding of HED diets resulted in a statistically significant increase in the TC cholesterol by 81% (Experiment 1) or 125% (Experiment 2), LDL cholesterol of 612% (Experiment 1) or 304% (Experiment 2) and a decrease in HDL cholesterol by 8.4%...
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Pharmacological Influence on Atherogenesis in Experimental Animal Models of Atherosclerosis
Rathouská, Jana ; Nachtigal, Petr (advisor) ; Vrablík, Michal (referee) ; Vohnout, Branislav (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Mgr. Jana Rathouská Supervisor: Doc. PharmDr. Petr Nachtigal, Ph.D. Title of Doctoral Thesis: Pharmacological influence on atherogenesis in experimental animal models of atherosclerosis Atherosclerosis is a slow inflammatory process in arterial walls which is, as a part of cardiovascular diseases, the main source of morbidity and mortality in developed countries. However, searching for the possibilities of atherosclerosis treatment requires detailed knowledge of pathogenesis of the disease itself. The use of mouse models offers one of the possibilities of studying the pathogenesis of atherosclerosis, which enables a number of interventions that would not be acceptable in human medicine. By using the high content lipid diet, we are able to induce the changes of lipid spectrum in mice. Moreover, if a targeted genetic manipulation is used, one can reach considerably advanced plaques in a relatively short time period. It is also possible to interfere in the process of atherogenesis in mouse models by using some hypolipidemic drugs, for example from the statin group that were represented in our studies by atorvastatin. By observing the vessel's reactivity to atorvastatin dosage,...
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