|
|
Application of modern analytical methods in clinical research
Vernerová, Andrea ; Kujovská Krčmová, Lenka (advisor) ; Doležal, Rafael (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training Workplace Department of Analytical Chemistry Doctoral Degree Program Bioanalytical Methods Candidate Mgr. Andrea Vernerová Supervisor Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Advisor MUDr. Ondřej Sobotka, Ph.D. Title of Doctoral Thesis Application of modern analytical methods in clinical research The dissertation thesis, composed as a compilation of five publications, is dealing with the development, optimization and validation of chromatographic methods and extraction procedures for the determination of early activation of the immune system biomarkers (neopterin, kynurenine, and tryptophan) in saliva and gingival crevicular fluid in oncological patients and patients with periodontal diseases. Furthermore, the potential of the use of Clark's sensor to determine the correct function of platelets by measuring mitochondrial respiration and its application in clinical research is under investigation. This work was based on cooperation with several clinical departments of the University Hospital Hradec Králové and Olomouc, and Oroboros Instruments (Innsbruck, Austria). The theoretical part of the thesis is focused on detail description of the individual analytes, their clinical significance, used biological matrices and their...
|
|
|
Application of modern analytical methods in clinical research
Vernerová, Andrea ; Kujovská Krčmová, Lenka (advisor) ; Doležal, Rafael (referee) ; Malíř, František (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training Workplace Department of Analytical Chemistry Doctoral Degree Program Bioanalytical Methods Candidate Mgr. Andrea Vernerová Supervisor Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Advisor MUDr. Ondřej Sobotka, Ph.D. Title of Doctoral Thesis Application of modern analytical methods in clinical research The dissertation thesis, composed as a compilation of five publications, is dealing with the development, optimization and validation of chromatographic methods and extraction procedures for the determination of early activation of the immune system biomarkers (neopterin, kynurenine, and tryptophan) in saliva and gingival crevicular fluid in oncological patients and patients with periodontal diseases. Furthermore, the potential of the use of Clark's sensor to determine the correct function of platelets by measuring mitochondrial respiration and its application in clinical research is under investigation. This work was based on cooperation with several clinical departments of the University Hospital Hradec Králové and Olomouc, and Oroboros Instruments (Innsbruck, Austria). The theoretical part of the thesis is focused on detail description of the individual analytes, their clinical significance, used biological matrices and their...
|
|
| |
|
|
Quantification of selected microRNAs-potential biomarkers of cardiotoxicity
Vernerová, Andrea ; Matoušková, Petra (advisor) ; Hanušová, Veronika (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Andrea Vernerová Supervisor: Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Determination of selected microRNA - potential cardiotoxicity biomarkers Cardiotoxicity is adverse reaction of chemotherapy that causes damage to the heart. Monitoring of potential biomarkers of cardiotoxicity could have a positive effect on the elimination of drugs' toxicity on heart tissue. Therefore, at present, interest in the potential microRNA (miRNA) as biomarkers for cardiotoxicity is rising. MiRNA is very stable short noncoding RNA that has the ability to post-transcriptionally regulate gene expression. From bioinformatic analyses miRNAs are able to regulate more than half of human genes. Various studies have shown miRNAs to be much more specific and rapid diagnostic biomarkers in comparison with troponins. MiRNAs as biomarkers are not established in routine clinical practice yet. So far, all studies of miRNAs are in the process of search and methods optimization. In my thesis selected miRNAs for detection of doxorubicin (DOX) cardiotoxicity in vivo in mouse cardiac tissue samples and in vitro on rat cardiomyocytes were investigated. RNA isolation from biological samples and reverse transcription, using...
|
|
| |
|
|
Ranking
Keřková, Gabriela ; Neumaierová, Inka (advisor) ; Vernerová, Andrea (referee)
The goal of diploma thesis is describing and evaluating of present, officially published rankings in the Czech Republic and creating own rankings in one branch of economy, specifically in health insurance industry. Evaluation is focused on quality and availability of information and used criteria, their significance and evaluators. I also focused on users of rankings and ways of measuring companies' efficiency, which is one of the most important criteria of rankings. Second part is focused on health insurance market. I describe subjects on this market, analyze share of different kinds of insurance on insurance globally and compare to Czech and European economy. I analyze evaluation of insurance companies by Czech national bank, which is authority for inspection of insurance market or rating agencies.
|
guest ::
RSS feed.