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Malignant Melanoma - New Scientific Knowledge
Woznica, Vlastimil ; Třešková, Inka (advisor) ; Hauer, Lukáš (referee) ; Měšťák, Ondřej (referee)
Introduction Malignant melanoma is one of the most aggressive skin tumors with unfavorable prognosis and high mortality, especially in the connection with advanced stages. Regarding the incidence growth, it has recorded an increase of 50 % in the last 10 years. The most important for prognosis of the patient with this malignant disease is early diagnosis and radical surgery without delay. In recent years, the benefit of targeted treatment and immunotherapy has been demonstrated in the patients with locally advanced melanoma after resection of primary tumor and nodal metastases. Aims The main goal of our study is to identify patients with a high risk of relapse using biomarkers from fluid biopsy (sections of free DNA circulating in blood) who went through a curative surgical treatment and for whom costly adjuvant therapy would be beneficial. Another goal is to use the above-mentioned biomarkers to detect early recurrence itself and residual disease after excision of the primary tumor site. Methods Whole blood samples were collected at regular time intervals from patients with early-stage malignant melanoma undergoing curative surgery. Plasma was first separated from the collected whole blood samples by two-phase centrifugation. Cell-free DNA (cfDNA) was isolated at the MD Anderson Cancer...
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Objective and subjective factors in the evaluation of outcomes of breast reconstruction after mastectomy
Tichá, Pavla ; Sukop, Andrej (advisor) ; Nejedlý, Aleš (referee) ; Třešková, Inka (referee)
For patients undergoing mastectomy, breast reconstruction provides an opportunity to restore their personal and social lives. During the last decade, there has been an upward trend in the number of successfully completed breast reconstructions. Reconstruction can be achieved by a number of methods which differ by their indication criteria as well as in their results. In addition to the objective analysis of clinical data, an integral part of the evaluation of these results should be a subjective evaluation of patient satisfaction with the surgery outcome and quality of life in the form of self- assessment questionnaires. Our specific objectives were to compare early postoperative and long-term clinical outcomes as well as patient-reported outcomes in terms of satisfaction and health-related quality of life (HRQoL) in patients undergoing implant-based reconstruction, abdominal-based autologous reconstruction, and combined reconstruction.
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Prognostic factors in malignant melanoma
Třešková, Inka ; Topolčan, Ondřej (advisor) ; Pizinger, Karel (referee) ; Nekulová, Miroslava (referee)
Background: Malignant melanoma is one of the most malignant types of skin cancer. Incidences are on the rise worldwide and in the Czech Republic an increase of 5% in diagnosed cases is noted each year. Early detection and early surgical removal are associated with reduced mortality. The strong aggressiveness of this malignant disease is caused by its local invasive growth and tendency to metastasize early. Aim of the study: The malignant melanoma is highly metabolically active tumor that releases a number of enzymes, cytokines, growth hormones and other molecules. The aim of this work was to determine the usability of preoperative and postoperative serum and plasma levels of biomarkers in primary diagnosis of tumor activity and in the postoperative follow-up care. These findings would be of clinical relevance for the patient's prognosis, modification of multimodal treatment and follow-up of patients with malignant melanoma. Methods: We measured circulating levels of several biomarkers in a group of 77 patients with malignant melanoma and cohort of 34 patients without cancer as a control group. Using routine immunoassays and novel multiplex xMAP technology, we measured: thymidine kinase, tissue polypeptide specific antigen, protein S100A, osteoprotegerin, osteopontin , insulin-like growth factor 1...
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Prognostic factors in malignant melanoma
Třešková, Inka ; Topolčan, Ondřej (advisor) ; Pizinger, Karel (referee) ; Nekulová, Miroslava (referee)
Background: Malignant melanoma is one of the most malignant types of skin cancer. Incidences are on the rise worldwide and in the Czech Republic an increase of 5% in diagnosed cases is noted each year. Early detection and early surgical removal are associated with reduced mortality. The strong aggressiveness of this malignant disease is caused by its local invasive growth and tendency to metastasize early. Aim of the study: The malignant melanoma is highly metabolically active tumor that releases a number of enzymes, cytokines, growth hormones and other molecules. The aim of this work was to determine the usability of preoperative and postoperative serum and plasma levels of biomarkers in primary diagnosis of tumor activity and in the postoperative follow-up care. These findings would be of clinical relevance for the patient's prognosis, modification of multimodal treatment and follow-up of patients with malignant melanoma. Methods: We measured circulating levels of several biomarkers in a group of 77 patients with malignant melanoma and cohort of 34 patients without cancer as a control group. Using routine immunoassays and novel multiplex xMAP technology, we measured: thymidine kinase, tissue polypeptide specific antigen, protein S100A, osteoprotegerin, osteopontin , insulin-like growth factor 1...
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