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Opioid-induced microbial dysbiosis
Popjak, Šimon ; Drastichová, Zdeňka (advisor) ; Stehlíková, Zuzana (referee)
The aim of this bachelor thesis is to describe the consequences of opioids in relation to the gut microbiome. Chronic opioid use induces microbial dysbiosis in the gut, where both functional and structural changes in the composition of the microbiome occur. This disrupts gut homeostasis and leads to diseases. The diversity of microbial organisms decreases and the expression of neurotransmitters changes. Opioids interfere with the microbiomeň-gut-brain axis, which normally functions in symbiosis. They alter the composition of the gut microbiota, affect the function and structure of the gut epithelium and the expression of neurotransmitters in the nervous system. This thesis focuses primarily on the action of morphine, the action of which has been desribed the most. Keywords: opioids, microbiome, dysbiosis, microbiome-gut-brain axis, gut homeostasis
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Cross-Country Analysis of Life Satisfaction
Stehlíková, Zuzana ; Janhuba, Radek (advisor) ; Paulus, Michal (referee)
This thesis examines the relations between subjective well-being and economic, politic and social characteristics of individual countries. We study the link between three components of subjective well-being - life satisfaction, positive affect and negative affect and other country characteristics. First, we use the dataset containing countries from almost all countries of the whole world and then with a more detailed European dataset. For the analysis of life satisfaction, panel data models are used. The results indicate that we are able to explain better variation in life satisfaction by our set of explanatory variables in comparison with positive affect and negative affect. In the former dataset, we discovered that the most important determinants of life satisfaction are GDP per capita, health, freedom to make life choices, political conditions and social support. In the latter, European dataset revealed a strong negative correlation between tertiary education attainment and happiness. The results also indicate that female representation in national parliament is an important predictor of life satisfaction in European countries. This supports the idea that equality between men and women plays an important role in developed societies. Keywords Subjective well-being, Happiness, Life satisfaction,...
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Image of old age and elderly care Institutions. Representation in daily newspaper Blesk
Stehlíková, Zuzana ; Šmídová, Olga (advisor) ; Trusinová, Romana (referee)
This diploma thesis deals with the representation and categorisation of old age and elderly care and their changes in media discourse of daily newspaper Blesk. Theory focuses on definitions of elderly and description of seniors and practices of elderly care in the Czech Republic. It describes changes of old age discourse in connection with the development of social policy and expert knowledge. It describes the concepts of representation and categorisation and their possible impacts in the form of stigmatisation, stereotyping and ageism. It discusses media as resources of symbolic power, ideology and discourse and sets out media representation of elderly. Related empirical part is based on qualitative discourse analysis of articles from Blesk in years 2007 and 2011. It discusses the outcomes with respect to changes of discourse and representation, stereotypes and active old age. It distinguishes three types of senior representation - like normal, stigmatized and active. Thematic distribution and stigmatisation of elderly constructs symbolic subordination of elderly as a population category. Care is described only rarely and isn't offered as the solution of the elderly's frailty.
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The transformations of belief and religious identity
Stehlíková, Zuzana ; Tichý, Radek (advisor) ; Řezáčová, Vendula (referee)
This bachelor thesis deals with the development of religious beliefs and identity in childhood and adolescence, as are described by young Catholics who lives in Prague. In theory, it focuses mainly on religious identity and its development in the late modern society and the current concepts of religion with regard to the Czech context. Empirical part is based on an analysis of semi-structured autobiographical interviews with young believers, in which two main phases of religious identity development appeared. In the first phase, situated in childhood, faith is taken for granted, and in the second, associated with adolescence, is seen as reflected choice. In conclusion, research results are compared with the theoretical basis.
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The Role of Microbiota in the Pathogenesis of Psoriasis
Stehlíková, Zuzana ; Jirásková Zákostelská, Zuzana (advisor) ; Demnerová, Kateřina (referee) ; Hrdý, Jiří (referee)
Psoriasis is a chronic, immune-mediated inflammatory skin disease. Its pathogenesis is associated with dysregulated cooperation among keratinocytes, innate and adaptive immune cells, coupled with environmental triggers, including microbiota. The aim of our study was to describe the microbiota composition in psoriasis and explore the role of bacteria and fungi in the pathogenesis of this disease. We used a mouse model of psoriasis induced by topical application of imiquimod (IISI) in both germ-free (GF) mice and conventional (CV) mice with microbiota manipulated by administration of a mixture of broad-spectrum antibiotics (ATB). ATB treatment markedly changed the intestinal but not the skin bacterial diversity and led to higher resistance to IISI in CV mice. Metronidazole was the most effective antibiotic, alleviating IISI symptoms in CV, but not in GF mice. This confirms that the effect of metronidazole on IISI was microbiota- dependent. Additionally, we characterized the microbiota composition of psoriatic lesions and unaffected skin in psoriatic patients compared to healthy controls, as well as the impact of different sampling approaches on uncovering cutaneous microbiota composition. We observed significant differences in α- and β-diversities when comparing identical samples sequenced on V1V2...
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Macroeconomic Uncertainty: An Exogenous Risk in Reinsurance Pricing
Stehlíková, Zuzana ; Kučera, Adam (advisor) ; Geršl, Adam (referee)
The thesis focuses on the analysis of the impact of the inflation uncertainty on the reinsurance pricing, particularly on its measures of risk. Vector autoregression models are used to predict the medium-term inflation and simulate different inflation paths. The consideration of various scenarios of future inflation captured by the stochastic modelling increases the value at risk (VaR) and the tail value of risk (TVaR) of mean ceded loss to the reinsurer. The thesis founds that the inflation uncertainty measured by the stochastic inflation matters and it is important from risk management and hedging perspectives. As a result, additional loadings could be added to the price for the mitigation of the inflation risk. Although the effect of stochasticity of the future inflation is not significant on mean loss, it is the case for the risk of measures, especially for the contracts with high retention relatively to the underlying exposure. JEL Classification F12, F21, F23, H25, H71, H87 Keywords reinsurance pricing, inflation forecasting, inflation risk, long-tail line of business Title Macroeconomic Uncertainty: An Exogenous Risk in Reinsurance Pricing
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Composition of skin microbiome in psoriatic patients
Stehlíková, Zuzana ; Jůzlová, P. ; Rob, F. ; Herzogová, J. ; Koren, O. ; Uzan, A. ; Tlaskalová-Hogenová, Helena ; Jirásková Zákostelská, Zuzana
Psoriasis is a chronic noninfectious and inflammatory skin disease, whose pathogenesis involves environmental triggers, including microbiota. In our study we compared bacterial composition between healthy controls and psoriatic patients using V1V2 hypervariable region of 16S rRNA. We found higher species diversity in psoriatic lesions than in contralateral psoriatic healthy site or in healthy control skin. Genus Propionibacterium was more abundant in contralateral healthy sites (57.77%) than in affected psoriatic sites of psoriatic patients (47.12%). On the other hand, we found higher abundance of genus Staphylococcus in psoriatic lesions (18.78%), while lower abundance in contralateral healthy sites (9.52%). Since Propionibacterium are commensal bacteria, the shift in their abundance from healthy to psoriatic skin could be due to disturbation of natural skin habitat. Interpretation of higher presence of Staphylococcus in psoriatic lesions comparing to contralateral healthy sites requires further species characterization. To complete the picture about psoriatic microbiome we will further investigate skin fungal composition in identical samples.
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Role of microbiota in mouse experimental model of psoriasis
Jirásková Zákostelská, Zuzana ; Stehlíková, Zuzana ; Klimešová, Klára ; Rossmann, Pavel ; Dvořák, Jiří ; Novosádová, Iva ; Kostovčík, Martin ; Coufal, Štěpán ; Šrůtková, Dagmar ; Hudcovic, Tomáš ; Štěpánková, Renata ; Rob, F. ; Jůzlová, P. ; Herzogová, J. ; Tlaskalová-Hogenová, Helena ; Kverka, Miloslav
Anotace v anglickém jazyce\n\nMouse model of human psoriasis and gnotobiotic are important tools in understanding the role of gut and skin microbiota in pathogenesis of psoriasis. In our experiments we showed that gnotobiotic mice, as well as conventional mice treated with antibiotics, have milder skin inflammation in comparison with control conventional mice. Treatment with broad spectrum antibiotics led to dramatic shift in gut microbial composition, in particular, we observed extensive increase of order Lactobacillales. To analyze the potential effect of Lactobacillales on skin inflammation, we further monocolonized mice with L. plantarum WCFS1. Also monocolonized mice showed lower skin inflammation in comparison with conventional mice. To understand whether microbial dysbiosis is cause or effect of psoriasis needs to be further investigated.\n\n
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Cross-Country Analysis of Life Satisfaction
Stehlíková, Zuzana ; Janhuba, Radek (advisor) ; Paulus, Michal (referee)
This thesis examines the relations between subjective well-being and economic, politic and social characteristics of individual countries. We study the link between three components of subjective well-being - life satisfaction, positive affect and negative affect and other country characteristics. First, we use the dataset containing countries from almost all countries of the whole world and then with a more detailed European dataset. For the analysis of life satisfaction, panel data models are used. The results indicate that we are able to explain better variation in life satisfaction by our set of explanatory variables in comparison with positive affect and negative affect. In the former dataset, we discovered that the most important determinants of life satisfaction are GDP per capita, health, freedom to make life choices, political conditions and social support. In the latter, European dataset revealed a strong negative correlation between tertiary education attainment and happiness. The results also indicate that female representation in national parliament is an important predictor of life satisfaction in European countries. This supports the idea that equality between men and women plays an important role in developed societies. Keywords Subjective well-being, Happiness, Life satisfaction,...
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Functional analysis of Spr1057 protein in Streptococcus pneumoniae
Stehlíková, Zuzana ; Branny, Pavel (advisor) ; Fišer, Radovan (referee)
Functional analysis of Spr1057 protein Streptococcus pneumoniae The genome of important human pathogen Streptococcus pneumoniae encodes a single gene of an eukaryotic type serine/threonine protein kinase StkP. Analysis of the global transcriptome of a mutant strain with inactivated stkP gene identified spr1057 gene whose expression was significantly repressed in ∆stkP strain. This gene is coding for Spr1057 protein which is a member of haloacid dehalogenase family. The analysis of the substrate specifity of the Spr1057 protein confirmed nucleotidase activity of this protein in vitro. To study the function of this protein in vivo we prepared several mutant S. pneumoniae strains. Growth characterictics of mutant strains were observed in the presence of modified nucleotides, 5-fluoro-2'-deoxyuridine (5-FdU) and 5-bromo-2'-deoxyuridine (5-BrdU). In addition, we monitored the rate of incorporation of 5-BrdU into the chromosomal DNA of the mutant strains in comparison with the wild type S. pneumoniae strain. The growth of the Δspr1057 strain was significantly inhibited in the presence of the modified nucleotides and increased incorporation of 5-BrdU in DNA was showed. Neither growth inhibition nor incorporation of 5-BrdU in DNA was observed for the wild type strain. The expression of an ectopic copy of spr1057...
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