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Suggestions for Improvement of Duties of Retail Mobile Operator Manager
Socha, Ondřej ; Šimeček, Filip (referee) ; Mikulec, Luděk (advisor)
This thesis is focused on the analysis of job duties leading mobile operator selected stores. Based on the analysis are proposed changes to those obligations in order to unify the concept of performance management stores and eliminate the difference in the performance of individual managers. In the thesis was to create a solution to increase the effectiveness of store Manager and improve performance stores including calculations of incurred costs and revenues.
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Development of new methodology for accurate determination of molecular geometry
Socha, Ondřej ; Dračínský, Martin (advisor) ; Munzarová, Markéta (referee) ; Hrabal, Richard (referee)
This thesis employs the combined strengths of Nuclear Magnetic Resonance (NMR) and computational methodologies to investigate chemical phenomena that influence molec- ular structure across the solid, liquid, and gas states. In the solid phase, the research focuses on chlorine quadrupole couplings, and their use in structure analysis, highlight- ing the advantages of NMR in conjunction with Density Functional Theory for data interpretation. In the liquid state, the tautomeric conversion of isocytosine is examined, underlining the role of NMR in analyzing intricate chemical exchanges. The gas phase study explores the dimerization of acetic acid using NMR, addressing previous inconsis- tencies and evaluating calculations of molecular structure and properties. The combined use of NMR and computational techniques has been instrumental in resolving existing discrepancies and providing a deeper understanding of these chemical systems. The find- ings underscore the importance of careful data interpretation and emphasise the strengths and constraints of the combined methodologies. 1
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Structural properties and stability of DNA hairpins characterized by NMR spectroscopy
Socha, Ondřej ; Římal, Václav (advisor) ; Srb, Pavel (referee)
CArG box is a highly conservative DNA motif found in Serum Response Element (SRE), which regulates expression of c-fos gene. In this thesis, short oligodeoxynucleotides containing CArG box were measured using nuclear magnetic resonance spectroscopy to evaluate their ability to preferentially form hairpin over duplex. 1H spectra were measured at temperature range (274-360) K. We acquired thermodynamic parameters of the transition between hairpin and single strand by fitting the temperature- dependent chemical shifts. The hairpin structure of our oligonucleotide samples was confirmed by non- B-DNA patterns in NOESY spectra, absence of concentration dependence of melting, and other pieces of evidence. Thus, occurrence of unusual DNA conformation of CArG box in native DNA, potentially even cruciform is highly possible. This could explain the high affinity between SRE and its transcription factor. Powered by TCPDF (www.tcpdf.org)
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Structural properties and stability of DNA hairpins characterized by NMR spectroscopy
Socha, Ondřej ; Římal, Václav (advisor) ; Srb, Pavel (referee)
CArG box is a highly conservative DNA motif found in Serum Response Element (SRE), which regulates expression of c-fos gene. In this thesis, short oligodeoxynucleotides containing CArG box were measured using nuclear magnetic resonance spectroscopy to evaluate their ability to preferentially form hairpin over duplex. 1H spectra were measured at temperature range (274-360) K. We acquired thermodynamic parameters of the transition between hairpin and single strand by fitting the temperature- dependent chemical shifts. The hairpin structure of our oligonucleotide samples was confirmed by non- B-DNA patterns in NOESY spectra, absence of concentration dependence of melting, and other pieces of evidence. Thus, occurrence of unusual DNA conformation of CArG box in native DNA, potentially even cruciform is highly possible. This could explain the high affinity between SRE and its transcription factor. Powered by TCPDF (www.tcpdf.org)
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Nuclear magnetic resonance study of local changes in DNA double-helix stability induced by cytosine methylation
Socha, Ondřej ; Římal, Václav (advisor) ; Kouřilová, Hana (referee)
Cytosine methylation in CpG dinucleotides plays an important role in regulation of gene expression and cell differentiation. The structure of DNA double-helix is only weakly affected by cytosine methylation in CpG containing oligonucleotides, but a significant change of the duplex stability is caused. In this thesis, the melting preoperties of two self-complementary oligonucleotides CAAC GTTG and CAAm5C GTTG are studied using nuclear magnetic resonance spectroscopy. We measured 1H spectra at temperature range (276-350)K. The equilibrium and kinetic parameters of chemical exchange between duplexes and single strands were obtained by fitting the spectral lineshapes. Methylation of cytosine in the central CpG dinucleotide increases the melting temperature of each base pair. Thus, a high degree of cooperativity during chemical exchange is observed in our samples. Powered by TCPDF (www.tcpdf.org)
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Suggestions for Improvement of Duties of Retail Mobile Operator Manager
Socha, Ondřej ; Šimeček, Filip (referee) ; Mikulec, Luděk (advisor)
This thesis is focused on the analysis of job duties leading mobile operator selected stores. Based on the analysis are proposed changes to those obligations in order to unify the concept of performance management stores and eliminate the difference in the performance of individual managers. In the thesis was to create a solution to increase the effectiveness of store Manager and improve performance stores including calculations of incurred costs and revenues.
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