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Holographic module for a light microscopy
Škrabalová, Denisa ; Slabý, Tomáš (referee) ; Dostál, Zbyněk (advisor)
The new arrangement of the off-axis holographic module, which is using polarizationactive diffraction grating divides signal into reference and subject wave of an interferometer based on their polarization. However, current design of the module does not have a possibility to tune a length of the optical paths. Thus the inability to tune optical paths leads to a reduced quality of interference structure during observation of biological samples. The current module is only suitable for technical applicating due to this limitation. Possibility of tuning branches is key step in biological applications. Therefore a new computer-controlled module is created in order to enable use for biological samples.
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Testing of multimodal holographic microscope in reflection mode
Kachtík, Lukáš ; Slabý, Tomáš (referee) ; Křížová, Aneta (advisor)
The thesis is focused on testing of reflection mode of Multimodal holographic microscope constructed in cooperation with TESCAN company. The assessment of axial and lateral resolution, verification of axial intensity response and documentation errors and deficiencies found while measuring are included in the testing procedure. In the conclusion, the Multimodal holographic microscope is compared to an atomic force microscop. A proper understanding of microscope operation and a formulation of an adjustment procedure were essential components of this work.
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Fresnel Incoherent Correlation Holography (FINCH)
Bouchal, Petr ; Slabý, Tomáš (referee) ; Chmelík, Radim (advisor)
In the Bachelor's thesis, the recently proposed method known as Fresnel Incoherent Correlation Holography (FINCH) is examined both theoretically and experimentally. Its main advantage consists in a possibility to realize holographic reconstruction of 3D objects illuminated by incoherent light. In FINCH, the object recording is performed applying methods of optical holography and digital diffractive optics. The object reconstruction is realized numerically and utilizes principles of digital holography. In experiments, the modern optoelectronic devices known as Spatial Light Modulators are effectively used. The Bachelor's thesis includes a short review including description of the basic principles of FINCH but its own contribution consists in the mathematical description of the method and creation of the numerical simulation model in Matlab. The main result of the thesis is design and realization of experiments enabling verification of the method. In the Bachelor's thesis, results of two independent experiments realized with different types of Spatial Light Modulators HOLOEYE and HAMAMATSU are presented. An agreement of experimental results with theoretical predictions is very good. A short discussion of the obtained results, further research topics and FINCH applications is also included in the Bachelors's Thesis.
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New Generation of a Coherence-Controlled Holographic Microscope
Slabý, Tomáš ; Novák,, Jiří (referee) ; Jákl, Petr (referee) ; Chmelík, Radim (advisor)
This doctoral thesis deals with design of a new generation of coherence-controlled holographic microscope (CCHM). The microscope is based on off-axis holographic configuration using diffraction grating and allows the use of temporally and spatially incoherent illumination. In the theoretical section a new optical configuration of the microscope is proposed and conditions for different parameters of the microscope and its optical components are derived. The influence of different sources of noise on phase detection sensitivity is studied. In the next section design of experimental setup is described and automatable adjustment procedure is proposed. Last section describes experimental verification of the most important optical parameters of the experimental setup. When compared to previous generation of CCHM, the newly proposed configuration uses infinity-corrected objectives and common microscope condensers, allows more space for the specimens, eliminates the limitation of spectral transmittance and significantly simplifies the adjustment procedure so that automation of this procedure is possible.
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Device for wavefront measurement of microscope objective lenses
Bartoníček, Jan ; Šerý, Mojmír (referee) ; Slabý, Tomáš (advisor)
The wavefront reconstruction of a light wave transformed by a microscope objective is the main subject of this diploma thesis together with the design and assembly of a~measuring device and the development of a computational algorithm. The purpose is to determine optical aberrations and to compare a quality of objectives with identical parameters. The term wavefront is explained and its description using Zernike polynomials is introduced in the first part of the thesis. The following part summarizes possible methods for wavefront reconstrucion. Two methods were chosen for experimental determination of a wavefront shape – shearing interferometry and solution of the transport of intensity equation. For each method a brief characteristic is provided together with possible applications, mathematical apparatus, image processing, computational procedure, setup description and proposition and results of experiments. The suitability of both methods for optical aberration determination and microscope objective comparison is discussed. Based on the obtained results, both methods were found to be suitable for comparison of microscope objectives. The suitability for optical aberration determination is possible with certain restrictions.
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Homogenized illumination source for microscopy
Riznerová, Petra ; Slabý, Tomáš (referee) ; Dostál, Zbyněk (advisor)
This thesis deals with the design of an illumination system for homogeneous illumination of the sample of a coherence-controlled holographic microscope. The theoretical part of the thesis briefly describes the principle of interference and holographic microscopy in connection with the use of a suitable illumination system for microscopy, including methods of light homogenization. The definition of the parameters of the new miniaturized source is based on this part. The work includes two designs of an illumination system with different use of microlens arrays. Optical simulations are performed, which verify the correct selection of all optical elements of the illumination system. Following the optical models, optomechanical designs are created. These are compiled and tested. The light efficiency of the sources and the distribution of light intensity values in their output planes are measured.
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Freedom to contract in employment relationship
Slabý, Tomáš ; Hůrka, Petr (advisor) ; Štefko, Martin (referee)
This thesis deals with the actual extent of contractual freedom in labor law relations from the theoretical perspective and from the perspective of its specific consequences in labor law as well. Therefore the thesis consists of two main parts. In part one, the thesis defines the legal principle concept and continues with the autonomy of the will characteristic, from which the contractual freedom principle derives, and then it describes its restrictions as a reset of both the principle of equality and the principle of protection of the weaker party to contract. As follows, the thesis deals with the position of labor law within the private law area, its historical development, the influence of the Constitutional Court decision no. 116/2008 Coll. and the adoption of the new Civil Code in terms of the labor law contractual freedom extent. The ending of the first part is dedicated to the modern "flexicurity" system that seeks to reach the balance between contractual freedom on one hand and an employee protection within labor law on the other hand. In its second part, the thesis aims to analyze particular appearances of contractual freedom within chosen labor law aspects as it both, shortly describes them and assesses them in terms of the space that they provide to contractual freedom and the amount of...
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Holographic module for a light microscopy
Škrabalová, Denisa ; Slabý, Tomáš (referee) ; Dostál, Zbyněk (advisor)
The new arrangement of the off-axis holographic module, which is using polarizationactive diffraction grating divides signal into reference and subject wave of an interferometer based on their polarization. However, current design of the module does not have a possibility to tune a length of the optical paths. Thus the inability to tune optical paths leads to a reduced quality of interference structure during observation of biological samples. The current module is only suitable for technical applicating due to this limitation. Possibility of tuning branches is key step in biological applications. Therefore a new computer-controlled module is created in order to enable use for biological samples.
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