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Fluorescence correlation spectroscopy in hydrophilic colloids
Rýcová, Eva ; Mondek, Jakub (referee) ; Venerová, Tereza (advisor)
This work is focused on studying of hydrophilic colloids using fluorescence correlation spectroscopy (FCS). The gel systems were studied with several fluorescent probes, in 0,15 M sodium chloride (NaCl) consist of hyaluronan and surfactant cetyltrimethylammonium bromide (CTAB). Probe ATTO 655 was chosen for closer examination due to the suppression of triplet state. Every surrounding occuring in the gel systems was examine individually. Results showed that the measurement of gel systems by this method can be realized under certain conditions, the repeatability of the results was loaded with a small standard deviation. Extension of diffusion times caused the presence of micelles, triplet state was caused by a characteristic environment of the hydrogel. The probe also showed free diffusion motion in all environments. This diffusion motion was represented by the shortest diffusion time.
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Diffusion properties of oppositely charged organic molecules in solutions of hydrophilic polyelectrolyte
Rýcová, Eva ; Márová, Ivana (referee) ; Mravec, Filip (advisor)
This work is focused on physical interactions of negatively charged polymers with small ionogenic fluorescent molecules. Trying to verify the presence of these interactions using fluorescence correlation spectroscopy (FCS) and provides a comprehensive view of the problem. The aim of this work is to observe the effect of concentration on the diffusion properties. P/D ratio, where P represents number of polymer binding sites and D number of dye binding sites, was chosen for this issue. Hyaluronate, sodium chondroitin sulfate and sodium polystyrene sulfonace were used as polymers and Acridine Orange, and Rhodamine 6G were chosen as fluorescent probes. The reason why this experiment uses these probes, was the assumption, that the positive charge occuring on the fluorescent probe will lead to the electrostatic interaction with the negatively charged polymer. As a result, the bond between acridine orange and polyelectrolyte was not clearly demonstrated, but the interaction with Rhodamine 6G have been proved.
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Diffusion properties of oppositely charged organic molecules in solutions of hydrophilic polyelectrolyte
Rýcová, Eva ; Márová, Ivana (referee) ; Mravec, Filip (advisor)
This work is focused on physical interactions of negatively charged polymers with small ionogenic fluorescent molecules. Trying to verify the presence of these interactions using fluorescence correlation spectroscopy (FCS) and provides a comprehensive view of the problem. The aim of this work is to observe the effect of concentration on the diffusion properties. P/D ratio, where P represents number of polymer binding sites and D number of dye binding sites, was chosen for this issue. Hyaluronate, sodium chondroitin sulfate and sodium polystyrene sulfonace were used as polymers and Acridine Orange, and Rhodamine 6G were chosen as fluorescent probes. The reason why this experiment uses these probes, was the assumption, that the positive charge occuring on the fluorescent probe will lead to the electrostatic interaction with the negatively charged polymer. As a result, the bond between acridine orange and polyelectrolyte was not clearly demonstrated, but the interaction with Rhodamine 6G have been proved.
|
|
|
Fluorescence correlation spectroscopy in hydrophilic colloids
Rýcová, Eva ; Mondek, Jakub (referee) ; Venerová, Tereza (advisor)
This work is focused on studying of hydrophilic colloids using fluorescence correlation spectroscopy (FCS). The gel systems were studied with several fluorescent probes, in 0,15 M sodium chloride (NaCl) consist of hyaluronan and surfactant cetyltrimethylammonium bromide (CTAB). Probe ATTO 655 was chosen for closer examination due to the suppression of triplet state. Every surrounding occuring in the gel systems was examine individually. Results showed that the measurement of gel systems by this method can be realized under certain conditions, the repeatability of the results was loaded with a small standard deviation. Extension of diffusion times caused the presence of micelles, triplet state was caused by a characteristic environment of the hydrogel. The probe also showed free diffusion motion in all environments. This diffusion motion was represented by the shortest diffusion time.
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