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Biblioverse – Multifunctional building with library
Mikulová, Veronika ; Kaftan, Martin (referee) ; Bykov, Oleksii (advisor)
The bachelor thesis deals with the design of a multifunctional building with a library in Brno on the corner of Štefánikova and Dřevařská streets. The main part of the building are the workshops, which accompany the visitor through the whole process of book creation, from paper making, through printing to book binding. The whole process of book creation is rounded off by the library, which houses a collection of books and other craft products created in these workshops. Nowadays, the library often becomes a cultural institution with a very broad focus, and the status of books sometimes takes a back seat. The aim of this work is not to criticize the current direction of these institutions and their interest in evolving with the times, but to try to highlight the book as a timeless and irreplaceable medium. The book is therefore seen as a medium that, in addition to its content value, also has the value of a physical arts and crafts object.
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Laboratory diagnostics of micrometastases in breast cancer patients
Mikulová, Veronika ; Zima, Tomáš (advisor) ; Svoboda, Marek (referee) ; Průša, Richard (referee)
Introduction: The presence of circulating tumor cells (CTC) in the peripheral blood has been associated with worse prognosis and early relapse in breast cancer patients. CTC determination in the peripheral blood has been considered as a liquid biopsy. The aim of this project was to analyze the presence of CTC followed by their molecular characterization with the potential use not only as a new biomarker for real-time monitoring of therapy efficacy but also as a suitable tool for patient's stratification and individualization of treatment for breast cancer. Methods: A total of 54 patients with diagnosed early breast cancer were enrolled into a prospective study. Ten millilitres of peripheral blood were sequentially collected to test for the presence and characterization of CTC during the follow-up of patients. CTC isolation and detection was performed by AdnaTest BreastCancer™ (AdnaGen AG, Germany), which is based on the detection of EpCAM, HER2 and MUC1 specific transcripts in enriched CTC- lysates. cDNA from isolated CTC has been further used for newly optimized qPCR assays for breast tumor and therapy resistance associated genes: TOP1, TOP2A, CSTD, ST6GAL, KRT19 and reference gene actin. qPCR results have been analyzed by Genex software (MultiD Analysis). Results: 195 blood samples have been...
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Laboratory diagnostics of micrometastases in breast cancer patients
Mikulová, Veronika ; Zima, Tomáš (advisor) ; Svoboda, Marek (referee) ; Průša, Richard (referee)
Introduction: The presence of circulating tumor cells (CTC) in the peripheral blood has been associated with worse prognosis and early relapse in breast cancer patients. CTC determination in the peripheral blood has been considered as a liquid biopsy. The aim of this project was to analyze the presence of CTC followed by their molecular characterization with the potential use not only as a new biomarker for real-time monitoring of therapy efficacy but also as a suitable tool for patient's stratification and individualization of treatment for breast cancer. Methods: A total of 54 patients with diagnosed early breast cancer were enrolled into a prospective study. Ten millilitres of peripheral blood were sequentially collected to test for the presence and characterization of CTC during the follow-up of patients. CTC isolation and detection was performed by AdnaTest BreastCancer™ (AdnaGen AG, Germany), which is based on the detection of EpCAM, HER2 and MUC1 specific transcripts in enriched CTC- lysates. cDNA from isolated CTC has been further used for newly optimized qPCR assays for breast tumor and therapy resistance associated genes: TOP1, TOP2A, CSTD, ST6GAL, KRT19 and reference gene actin. qPCR results have been analyzed by Genex software (MultiD Analysis). Results: 195 blood samples have been...
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Restitution process according to law 229/1991 emphatically on ecclesiastic restitution
Mikulová, Veronika ; Zeman, Karel (advisor) ; Vebrová, Ludmila (referee)
Bachelor's thesis will focus on the restitution process, emphatically on ecclesiastic restitutions in the Czech Republic. This topic is very actual and discussed today, because at present Church still makes new restitution claims, however on a little different way than there is solved settlement of "common competent persons" claims. In the theoretical part there will be briefly described overall transformation of the national economy. Main emphasis will be placed on an explanation of restitution process principles, namely in three legal regimes in the Czech Republic within systemic changes of the national economy. In the second part, analytical, there will be main view to restitution process development in the Czech Republic according to law 229/1991. Further I will focus on an analysis of differences during restitutions of "common people" and restitutions of Churhes possesions. I will try to find the answer on the question if required Church demands are according to valid laws of the Czech Republic, to Declaration of Basic Rights, eventually to the other standards. In the final part I will evaluate Church procedure in the restitution process, I will investigate if there are not happening atypical claims setting up during property composition, what will be main point of this work.
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