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ZIP7, hlavní místo intracelulární signalizace zinku: studium prostřednictvím mutageneze
Manišová, Michaela ; Pávek, Petr (advisor) ; Skálová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Michaela Manišová Supervisors: Dr. Kathryn Taylor, Ph.D. Doc. PharmDr. Petr Pávek, Ph.D. Title of diploma thesis: ZIP7, a hub for intracellular zinc signalling. Functional investigation by site-directed mutagenesis. Zinc is essential for many cellular processes. It is cofactor for enzymes, important for normall cell growth and can be classified as a second messenger. A significant role of zinc is ability to inhibit protein tyrosine phosphatases activity, resulting in activation of mitogen-activated protein kinases. Zinc is unable passively cross over the cell membranes and need special transporters. Zinc transporters have a main role in intracellular zinc homoeostasis, aberrations of which could lead to diseases such as cancer. The zinc transporter solute carrier family 39, member 7 (SLC39A7, commonly referred to as ZIP7) releases zinc from the endoplasmic reticulum into the cytoplasm and might be required for tyrosine kinases activation. ZIP7 is encoded by 1407 nucleotide pairs which fit into 469 amino acids. ZIP7 contains 8 transmembrane domains and two predicted phosphorylation residues one at serine 275 and one at serine 276. The presence of these phosphorylation sites is an...
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Pharmacovigilance: Drug regulation development and legislation overview.
Manišová, Michaela ; Kostřiba, Jan (advisor) ; Zimčíková, Eva (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Social and Clinical Pharmacy Candidate Mgr. Michaela Manišová Consultant PharmDr. Jan Kostřiba, Ph.D. Title of Thesis Pharmacovigilance: Drug regulation development and legislation overview. Introduction: Pharmacovigilance is a scientific discipline relating to the detection and evaluation of adverse drug reactions and other risks associated with the use of the medicinal products after marketing authorisation and implementation of the risk minimisation measures. Objectives: To analyze the legislation requirements in the area of pharmacovigilance for the medicinal products for human use in the European Union and their implementation in practice. Trends in adverse drug reactions reporting in the various pharmacovigilance systems (United States, Czech Republic, European Union), evaluation of the factors affecting reporting and the main benefits of spontaneous reporting. Methods: The legal requirements of the European Union, the Czech Republic and the United States was used as the source of information, followed by the official website of the Drug Regulatory Authorities, the best practices guidelines prepared by the European Medicines Agency and by the Food and Drug Administration, the harmonization guidelines issued by...
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Pharmacovigilance: Drug regulation development and legislation overview.
Manišová, Michaela ; Kostřiba, Jan (advisor) ; Zimčíková, Eva (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Social and Clinical Pharmacy Candidate Mgr. Michaela Manišová Consultant PharmDr. Jan Kostřiba, Ph.D. Title of Thesis Pharmacovigilance: Drug regulation development and legislation overview. Introduction: Pharmacovigilance is a scientific discipline relating to the detection and evaluation of adverse drug reactions and other risks associated with the use of the medicinal products after marketing authorisation and implementation of the risk minimisation measures. Objectives: To analyze the legislation requirements in the area of pharmacovigilance for the medicinal products for human use in the European Union and their implementation in practice. Trends in adverse drug reactions reporting in the various pharmacovigilance systems (United States, Czech Republic, European Union), evaluation of the factors affecting reporting and the main benefits of spontaneous reporting. Methods: The legal requirements of the European Union, the Czech Republic and the United States was used as the source of information, followed by the official website of the Drug Regulatory Authorities, the best practices guidelines prepared by the European Medicines Agency and by the Food and Drug Administration, the harmonization guidelines issued by...
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ZIP7, hlavní místo intracelulární signalizace zinku: studium prostřednictvím mutageneze
Manišová, Michaela ; Skálová, Lenka (referee) ; Pávek, Petr (advisor)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Michaela Manišová Supervisors: Dr. Kathryn Taylor, Ph.D. Doc. PharmDr. Petr Pávek, Ph.D. Title of diploma thesis: ZIP7, a hub for intracellular zinc signalling. Functional investigation by site-directed mutagenesis. Zinc is essential for many cellular processes. It is cofactor for enzymes, important for normall cell growth and can be classified as a second messenger. A significant role of zinc is ability to inhibit protein tyrosine phosphatases activity, resulting in activation of mitogen-activated protein kinases. Zinc is unable passively cross over the cell membranes and need special transporters. Zinc transporters have a main role in intracellular zinc homoeostasis, aberrations of which could lead to diseases such as cancer. The zinc transporter solute carrier family 39, member 7 (SLC39A7, commonly referred to as ZIP7) releases zinc from the endoplasmic reticulum into the cytoplasm and might be required for tyrosine kinases activation. ZIP7 is encoded by 1407 nucleotide pairs which fit into 469 amino acids. ZIP7 contains 8 transmembrane domains and two predicted phosphorylation residues one at serine 275 and one at serine 276. The presence of these phosphorylation sites is an...
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