|
|
Development and differentiation of different types of yeast colonies: Regulation of metabolic diversification and development of cells with novel properties
Maršíková, Jana
Yeasts are unicellular organisms, but on a solid substrate they are capable of forming complex organized structures that behave like primitive multicellular organisms. Examples of these structures include colonies and biofilms, whose cells interact with each other, coordinate their growth and development, differentiate spatially and form specialized cell subpopulations in which specific processes and regulatory pathways occur. The basis of cellular differentiation and specialization is the formation of gradients of nutrients, metabolites and signaling molecules. Thus, multicellular yeast communities differ significantly from planktonic populations in their characteristics. The aim of this work is to increase knowledge related to the development and differentiation of both smooth and structured colonies of the yeast Saccharomyces cerevisiae. The literature introduction of the thesis provides an overview of the current knowledge on the development of yeast colonies and biofilms, especially of S. cerevisiae species, and also includes selected regulations important for the formation of multicellular populations. The thesis provides insights into the antagonistic function of the transcriptional regulators Cyc8p and Tup1p in the development of structured biofilm colonies. Genome-wide transcriptomic...
|
|
|
Development and differentiation of different types of yeast colonies: Regulation of metabolic diversification and development of cells with novel properties
Maršíková, Jana ; Palková, Zdena (advisor) ; Heidingsfeld, Olga (referee) ; Demnerová, Kateřina (referee)
Yeasts are unicellular organisms, but on a solid substrate they are capable of forming complex organized structures that behave like primitive multicellular organisms. Examples of these structures include colonies and biofilms, whose cells interact with each other, coordinate their growth and development, differentiate spatially and form specialized cell subpopulations in which specific processes and regulatory pathways occur. The basis of cellular differentiation and specialization is the formation of gradients of nutrients, metabolites and signaling molecules. Thus, multicellular yeast communities differ significantly from planktonic populations in their characteristics. The aim of this work is to increase knowledge related to the development and differentiation of both smooth and structured colonies of the yeast Saccharomyces cerevisiae. The literature introduction of the thesis provides an overview of the current knowledge on the development of yeast colonies and biofilms, especially of S. cerevisiae species, and also includes selected regulations important for the formation of multicellular populations. The thesis provides insights into the antagonistic function of the transcriptional regulators Cyc8p and Tup1p in the development of structured biofilm colonies. Genome-wide transcriptomic...
|
|
|
Crosstalks between nitrogen metabolism, the TOR pathway and the metabolism of lipids
Grulyová, Michaela ; Převorovský, Martin (advisor) ; Maršíková, Jana (referee)
Cells coordinate their metabolism based on various factors, for example nitrogen availability. The TOR pathway is an important regulator of nitrogen metabolism, it has a role in sensing intracellular amino acids status, and it controls especially cell growth, protein synthesis, proliferation and cell survival. However, it has been shown that the TOR pathway also controls lipid biosynthesis and lipid accumulation through various mechanisms in response to nitrogen availability. Although the TOR pathway is well conserved among the eukaryotic organisms, its outcomes differ diametrically when it comes to the lipid accumulation. This essay provides some insides into the mechanisms of regulation of the lipid metabolism by the TOR pathway.
|
|
|
The effect of HAc1p on the development of yeast colony
Maršíková, Jana ; Schierová, Michaela (advisor) ; Pichová, Iva (referee)
On solid surfaces wild strains of Saccharomyces cerevisiae form biofilm-like, structured colonies enabling them to survive long-term in hostile environments in the wild. However, the molecular mechanisms underlying the spatio-temporal development of colonies and their resistance to hostile conditions are still largely unknown. In this study, we analyzed the effect of the HAC1 gene on the colony morphology of wild strains of S. cerevisiae. The transcription factor Hac1p activates the unfolded protein response (UPR), which leads to activation of the expression of genes encoding components of the protein secretory pathway, and genes involved in stress responses in the endoplasmic reticulum (ER). The impact of HAC1 deletion is significant even under non-stress conditions and causes a radical reduction of structured colony architecture in hac1∆ strains derived from two wild S. cerevisiae strains (PORT and BR-F-Flo11p-GFP) and one laboratory ΣSh strain forming semi-fluffy or fluffy colonies. The hac1∆ strains exhibit a decreased vegetative growth rate, reduced cell attachment to the agar and an ineffective cell-cell adhesion resulting in decreased flocculation. The hac1∆ strains derived from BR-F-Flo11p-GFP contain a low level of Flo11p surface adhesin which is considered very important for the proper...
|
|
|
A Gem From non-Traditional Materials
Maršíková, Jana ; Cikánová, Karla (referee) ; Havlásek Tatarová, Lucie (advisor)
The title of the graduation thesis is a gem from non-traditional materials. The first part of the thesis focuses on the erratic c arac of the gem and its function with respect to time and space.The second part describes events connected with Czech author's gem in broader cultural and political connexions. The third part is devoted to individual and traditional materials used during production of a gem and authors who use these materials.The pedagogical part deals with a gem in the lessons of Arts and Craft. Artistic suggestions were partially realized at Elementary School Na Chodovci and their reflection is elaborated in the pedagogical part of the t esis. The last part of the thesis describes a draft of author's own The whole work is supplemented with large amount of illu satrrta wtivoer kms a(ttherrieaels t. imes collection of gems from non-traditional materials). Key words: a gem, a material, non-traditional materials, arts and crafts, design, art education
|
|
|
Urinary Inkontinence as a Stress Faktor on Seniors- Women
MARŠÍKOVÁ, Jana
Abstract Urinary incontinence is involuntary leakage of urine, which causes medical, psychological, social and hygienic problems. Incontinence is a common problem of senior age and influences all fields of women´s life. Almost every senior woman has some kind of experience with this issue, either personal or linked to someone she knows. This thesis deals with causes of incontinence origin, its diagnostics and treatment possibilities. The aim was to find out whether incontinence influences mental condition of senior women and if so, in which way the problems connected with the incontinence are present. The thesis further examines how senior women cope with information about their problems, whether they have sufficient information and whether the information is understandable. It is also important to know what the modern-day options for treatment of this problem are, and whether senior women are willing to undergo the procedures and examinations. The thesis also deals with the question whether senior women have sufficiency of incontinency aids, whether they are satisfied with the nursing care and whether their privacy during sanitation is satisfactory. The survey shows that senior women are stressed by urine leakage, they reduce their activities, socially isolate themselves, are dissatisfied with the quantity of the incontinency aids, limit the fluid intake. The finding, that senior women are not much interested in receiving more information, in further detailed examinations, or in pelvic muscle rehabilitation, was surprising. It has been proven that doctors offer mainly pharmacological and compensational treatment to senior women. Regarding the privacy issue, it has been proven that senior women are satisfied with the nursery care and have adapted to the lack of privacy. It resulted from the survey that nurses do not have enough professional information for education of senior women. Elaboration of a new educational plan for the Senior House clients and nurses working in the facility is the output of this thesis.
|
guest ::
RSS feed.