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Thermophilic Enzymes
Kuchtová, Ludmila ; Obruča, Stanislav (referee) ; Babák, Libor (advisor)
Thermophilic enzymes are produced by organisms living at temperatures between 60 and 80°C, in some cases even higher, and are active and stabile at these temperatures. This work deals with thermophilic and hyperthermophilic organisms, their habitats, ways of adaptation to environment with considerably higher temperatures than optimal temperature for most organisms, and recent findings and opinions about possible factors enhancing protein thermostability. Properties and behaviour of thermophilic enzymes and their current applications (i.e. PCR, starch industry) are summarized. Advantages and disadvantages of using of these enzymes in comparison to currently used mesophilic enzymes are compared. Possible applications of thermozymes in various industrial branches, where they could replace some environment polluting procedures, simplify technology and be economically important, are introduced.
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Growth characteristics of thermophilic microorganisms
Kuchtová, Ludmila ; Vítová, Eva (referee) ; Babák, Libor (advisor)
The aim of this work was focused on study of influence of cultivation conditions on growth of thermophilic bacteria Thermus aquaticus, Thermus scotoductus, Geobacillus thermodenitrificans and Geobacillus thermocatenulatus in cultivation medium recommended by Czech Collection of Microorganisms (CCM). The change of concentration of biomass during cultivation with various pH of media, cultivation temperature, agitation rate and with addition of glucose to medium during cultivation in Erlenmayer flasks was observed. Results served for determination of optimal growth conditions for each microorganism. After optimal growth conditions were obtained, cultivations in fermenter were performed. Growth curves were made from values of absorbance and dry weight of samples.
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The roofing of amphitheatre in Vizovice
Kuchtová, Ludmila ; Štrba, Michal (referee) ; Šmak, Milan (advisor)
The scope of this bachelor’s thesis is to design roofing over a natural amphitheater stage in Vizovice. The structure is designed as a variable structure in verison of both steel and timber. Each solution has a different structural composition. The layout is triangular with width of 18 meters and length of 15 meters. The height of the roofing is considered to be 9 meters.
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The botanical pavillion in Jihlava
Kuchtová, Ludmila ; Buchta, Stanislav (referee) ; Šmak, Milan (advisor)
The aim of this diploma thesis is to design a load-bearing structure of the botanical pavillion in Jihlava. The construction is designed as a variable structure of both steel and timber. Each solution has a different type of ribs. The layout is a hexadecagon with maximum dimension of 30 meters. The height of the building is considered to be 10 meters.
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The botanical pavillion in Jihlava
Kuchtová, Ludmila ; Buchta, Stanislav (referee) ; Šmak, Milan (advisor)
The aim of this diploma thesis is to design a load-bearing structure of the botanical pavillion in Jihlava. The construction is designed as a variable structure of both steel and timber. Each solution has a different type of ribs. The layout is a hexadecagon with maximum dimension of 30 meters. The height of the building is considered to be 10 meters.
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The roofing of amphitheatre in Vizovice
Kuchtová, Ludmila ; Štrba, Michal (referee) ; Šmak, Milan (advisor)
The scope of this bachelor’s thesis is to design roofing over a natural amphitheater stage in Vizovice. The structure is designed as a variable structure in verison of both steel and timber. Each solution has a different structural composition. The layout is triangular with width of 18 meters and length of 15 meters. The height of the roofing is considered to be 9 meters.
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Growth characteristics of thermophilic microorganisms
Kuchtová, Ludmila ; Vítová, Eva (referee) ; Babák, Libor (advisor)
The aim of this work was focused on study of influence of cultivation conditions on growth of thermophilic bacteria Thermus aquaticus, Thermus scotoductus, Geobacillus thermodenitrificans and Geobacillus thermocatenulatus in cultivation medium recommended by Czech Collection of Microorganisms (CCM). The change of concentration of biomass during cultivation with various pH of media, cultivation temperature, agitation rate and with addition of glucose to medium during cultivation in Erlenmayer flasks was observed. Results served for determination of optimal growth conditions for each microorganism. After optimal growth conditions were obtained, cultivations in fermenter were performed. Growth curves were made from values of absorbance and dry weight of samples.
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|
|
Thermophilic Enzymes
Kuchtová, Ludmila ; Obruča, Stanislav (referee) ; Babák, Libor (advisor)
Thermophilic enzymes are produced by organisms living at temperatures between 60 and 80°C, in some cases even higher, and are active and stabile at these temperatures. This work deals with thermophilic and hyperthermophilic organisms, their habitats, ways of adaptation to environment with considerably higher temperatures than optimal temperature for most organisms, and recent findings and opinions about possible factors enhancing protein thermostability. Properties and behaviour of thermophilic enzymes and their current applications (i.e. PCR, starch industry) are summarized. Advantages and disadvantages of using of these enzymes in comparison to currently used mesophilic enzymes are compared. Possible applications of thermozymes in various industrial branches, where they could replace some environment polluting procedures, simplify technology and be economically important, are introduced.
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