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Sexual promiscuity, cryptic female choice and the biology of passerine sperm
Míčková, Kristýna ; Albrecht, Tomáš (advisor) ; Vrbacký, Marek (referee) ; Krist, Miloš (referee)
The discovery of postcopulatory mechanisms of mate choice has fundamentally altered our understanding of sexual selection. Passerines are an ideal group for studying two basic mechanisms of postcopulatory sexual selection, sperm competition and cryptic female choice. This doctoral thesis focuses on postcopulatory sexual selection in passerines from three different angles. In this study, we investigated (1) age-related changes in traits that can affect male reproductive success (sperm morphology, velocity and production) and a trade-off between these traits and lifespan in male barn swallows (Hirundo rustica); (2) variation in seminal fluid and sperm proteins in six selected passerine species and whether these differences are caused by phylogeny or postcopulatory sexual selection; and (3) the protein composition of female barn swallow cloacal fluids in four phases of the reproductive season, changes in protein composition in individual phases, and identification of phase-specific proteins. Our results show that there is no unified trend for changes in sperm traits or sperm production across male lifespan. While sperm morphology and velocity do not change considerably, sperm production increases in first years of life. However, excessive investment in sperm production could reduce the male's chances...
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Effect of incubation temperature on phenotype in mallard
Cílková, Martina ; Hořák, David (advisor) ; Krist, Miloš (referee)
The influence of incubation temperature on bird neonate phenotype is widely studied. In waterfowl (Anatidae), maternal effect can be divided to two main parts: clutch formation and its incubation. Therefore, waterfowl are a good model for this type of study. The egg temperature can strongly influence embryo development and survival of the hatchlings. The goal of this work was to examine the effect of incubation temperature on hatchability, incubation length, sex ratio and phenotype of European Mallard ducklings immediately after hatching. Eggs were randomly placed in one of six incubation temperatures, which ranged from 35 to 39 řC. This range corresponds with temperature range in natural mallard nests in the Czech Republic. Eggs were then artificially incubated. Newborn hatchlings were weighted and their structural size was measured, then they were killed. Some hatchlings from incubation temperatures 35.5, 36 and 37 řC were used to examine residual yolk sac mass and basic chemical composition of the yolk-free body. Hatchability was markedly lower in both extreme temperatures (35 and 39 řC). Incubation length significantly decreased with increasing incubation temperature. I failed to prove the influence of incubation temperature on hatchling body mass, but I found temperature-dependent changes of...
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Temperature-dependent activity of egg-white antimicrobial proteins in precocial and altricial birds
Novotná, Lucie ; Javůrková, Veronika (advisor) ; Krist, Miloš (referee)
Microorganisms are considered to be crucial selective factor affecting reproductive success of birds. It is hypothesized that egg-white antimicrobial proteins and incubation behavior are the most important defense mechanisms that eliminates the risk of microbial trans-shell infection. The latest studies supposed that incubation temperatures may significantly affect the antimicrobial activity of egg white proteins. The concentration of egg white antimicrobial proteins as well as incubation patterns differ among altricial and precocial species of birds. However, experimental study testing the effect of incubation temperature on the antimicrobial potential of antimicrobial egg white proteins in altricial and precocial birds is missing. In this study we tested in manipulative experiment the effect of partial and full incubation, the concentration of lysozyme and ovotransferrin and their interactions on the antimicrobial activity of egg whites of two model species - Japanese quail (Coturnix japonica) and home pigeon (Columba livia). Antimicrobial protein activity was determined by the agar well diffusion method against two Gram-positive bacteria - Bacillus subtilis and Micrococcus luteus that have been documented as pathogenic egg-white invaders of several birds. Moreover, we analyzed the effect of...
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The role of interspecific competition in ecological differentiation and speciation in two passerine species, Luscinia megarhynchos and Luscinia luscinia
Sottas, Camille Pauline ; Reifová, Radka (advisor) ; Krist, Miloš (referee) ; Sedláček, Ondřej (referee)
(in English) Interspecific competition is assumed to play an important role in the morphological and ecological differentiation of co-occurring species and could contribute to their speciation. However, empirical studies showing a direct association between competition, ecological niche divergence and specific morphological adaptations are scarce. This thesis focuses on the role of interspecific competition in the differentiation and the evolution of reproductive isolation between two closely related passerine species: the common nightingale (Luscinia megarhynchos) and the thrush nightingale (Luscinia luscinia). Both species are insectivorous and have similar ecological niches. They have diverged 1.8 Mya and their areas currently overlap in a secondary contact zone, where both species still occasionally hybridize. Previous studies have reported an accentuate bill size divergence in sympatric populations compared to allopatric populations, suggesting that interspecific competition might have resulted in feeding niche divergence between the two species in sympatry. Within this PhD thesis, I have studied potential associations between bill morphology divergence, diet composition, habitat use and gut microbiota composition. We found that the two nightingale species have diverged in feeding niche in...
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Temperature-dependent activity of egg-white antimicrobial proteins in precocial and altricial birds
Novotná, Lucie ; Javůrková, Veronika (advisor) ; Krist, Miloš (referee)
Microorganisms are considered to be crucial selective factor affecting reproductive success of birds. It is hypothesized that egg-white antimicrobial proteins and incubation behavior are the most important defense mechanisms that eliminates the risk of microbial trans-shell infection. The latest studies supposed that incubation temperatures may significantly affect the antimicrobial activity of egg white proteins. The concentration of egg white antimicrobial proteins as well as incubation patterns differ among altricial and precocial species of birds. However, experimental study testing the effect of incubation temperature on the antimicrobial potential of antimicrobial egg white proteins in altricial and precocial birds is missing. In this study we tested in manipulative experiment the effect of partial and full incubation, the concentration of lysozyme and ovotransferrin and their interactions on the antimicrobial activity of egg whites of two model species - Japanese quail (Coturnix japonica) and home pigeon (Columba livia). Antimicrobial protein activity was determined by the agar well diffusion method against two Gram-positive bacteria - Bacillus subtilis and Micrococcus luteus that have been documented as pathogenic egg-white invaders of several birds. Moreover, we analyzed the effect of...
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Physiological aspects of the variability in eggshell pigmentation
Mengr, Jan ; Javůrková, Veronika (advisor) ; Krist, Miloš (referee)
The avian eggshell colouration is caused by specific pigments deposited into the eggshell. These pigments differ in colour, chemical structure and the eggshell deposition place. The function of the eggshell pigmentation is traditionally considered to have cryptic or thermoregulatory role. However, most recent hypotheses provide a new perspective on the function of eggshell pigmentation. As between species variability in the eggshell pigmentation is under strong genetic control, within species variability is considered to be a result of adaptation to environmental factors affecting physiology of female bird. This work focuses on the description and characteristics of particular eggshell pigments, their synthesis, deponation process and also the importance of variability in the avian eggshell pigmentation and its physiological aspects. Powered by TCPDF (www.tcpdf.org)
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Effect of incubation temperature on phenotype in mallard
Cílková, Martina ; Hořák, David (advisor) ; Krist, Miloš (referee)
The influence of incubation temperature on bird neonate phenotype is widely studied. In waterfowl (Anatidae), maternal effect can be divided to two main parts: clutch formation and its incubation. Therefore, waterfowl are a good model for this type of study. The egg temperature can strongly influence embryo development and survival of the hatchlings. The goal of this work was to examine the effect of incubation temperature on hatchability, incubation length, sex ratio and phenotype of European Mallard ducklings immediately after hatching. Eggs were randomly placed in one of six incubation temperatures, which ranged from 35 to 39 řC. This range corresponds with temperature range in natural mallard nests in the Czech Republic. Eggs were then artificially incubated. Newborn hatchlings were weighted and their structural size was measured, then they were killed. Some hatchlings from incubation temperatures 35.5, 36 and 37 řC were used to examine residual yolk sac mass and basic chemical composition of the yolk-free body. Hatchability was markedly lower in both extreme temperatures (35 and 39 řC). Incubation length significantly decreased with increasing incubation temperature. I failed to prove the influence of incubation temperature on hatchling body mass, but I found temperature-dependent changes of...
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The role of nest in reproduction of the Great Reed Warbler
Jelínek, Václav ; Procházka, Petr (advisor) ; Krist, Miloš (referee) ; Trnka, Alfréd (referee)
7 Abstract Bird nests protect eggs and nestlings, allowing the parents to leave their offspring and subsequently return to them. Their thermoregulatory properties reduce energetic costs of incubation and brooding of nestlings. For all these reasons, nests are key structures for the reproduction of a majority of avian species and as such they should be subject to natural selection. Several hypotheses describing selection pressures which affect the size of nests or some of their parts have been suggested. In my PhD thesis, I investigated some of them in the great reed warbler (Acrocephalus arundinaceus) - an open nesting passerine species with very variable nest size. For this purpose, I used a large dataset of several hundred measured great reed warbler nests, nest enlargement experiments and an experiment with artificial nests. In accordance with previous studies, we did not find that nest size affects the probability of common cuckoo (Cuculus canorus) brood parasitism, while it was influenced by other factors, such as timing of breeding, reed density around the nest and nest visibility from the nearest potential cuckoo perch site. More interestingly, we found that cuckoos adjust their nest-searching strategy in relation to availability of host nests. When host nests were scarce, cuckoos parasitized all of...
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