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Detection of tones from audio signal by signal processing methods
Kristek, Lukáš ; Doležal, Jan (referee) ; Veselý, Karel (advisor)
This bachelor's thesis focuses on tone detection from audio signals using signal processing methods and evaluating their performance with F1 metric. The methods used include the Fourier transform (FT), Constant Q-transform (CQT). As an extension of the thesis we experiment with our own method based on the presence of harmonic tones, which internally uses the Fourier transform. Each method has a specific procedure for the final decision of a tone detection. The thesis is focused on the implementation of these procedures and the comparative evaluation of the individual methods.
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Toxicity of nanotextiles as respirators waste to Daphnia Magna
Slaný, Jan ; Roupcová, Petra ; Pavlovský, Jiří ; Klouda, Karel
In recent years, the prevalence of nanotubes has increased due to the covid-19 disease situation worldwide. Nanotextiles are used in the form of face masks and respirators, which are a source of environmental pollution by nanoparticles. The aim of this paper is to perform ecotoxicological tests according to European standards and Czech legislation. First, non-woven fabrics are briefly described and a small planktonic crustacean (Daphnia magna S.) is introduced. The second part focuses on the experiments themselves and their results. In all cases, the nanotextiles tested did not have a toxic effect on the organisms, but a reaction could be observed. Further tests will be carried out to explain and clarify the effects.
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Funeral Cast Iron plastics from the Brdy Region examined considering its Contacts with Cast Iron Manufacturing Centres in Prussia and Moravia in the 19th Century
Bělová, Jana ; Royt, Jan (advisor) ; Otavský, Karel (referee) ; Prahl, Roman (referee)
Funeral cast iron plastics offer an unique insight into the blending of art and trade in the 19th century. The Brdy region represented a very important centre of iron manufacture at that time, from which artistic ingenuity as well as skilled trade spread. The project Funeral cast iron plastics from the Brdy Region examined considering its Contacts with Cast Iron Manufacturing Centres in Prussia and Moravia in the 19th Century presents the Brdy cast iron within the context of the most important production centres of cast iron in Prussia, Moravia and Austria. 39 sample books were examined and described and photographic documentation of 2020 funeral monuments was gathered. 547 of these were documented in Berlin, 142 in Gliwice, 559 in and around Hořovice, Beroun, Rožmitál, Plzeň, and Plasy, 376 in the region of Blansko and Frýdlant nad Ostravicí, in and around Mariazell, pictures of 396 monuments were taken. Funeral cast iron plastics stand between artistic and commercial cast iron production. Three areas of Funeral cast iron Production in central Europe were identified and examined. The first one arose from the Prussian foundries in Berlin and Gliwice. The foundry of Plasy and also the Fürstenberk foundry in Beroun as well as the older production of the Blansko foundry are linked to the heritage of...
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Role of signaling pathways and redox processes in cancer cell biology
Vališ, Karel ; Kovář, Jan (advisor) ; Anděra, Ladislav (referee) ; Kozubík, Alois (referee)
5 identifikovali VDAC2 protein jako možný transportér železa do nádorových buněk a tudíž možný cíl protinádorové terapie. Abstract Specific apoptosis induction in cancer cells represents promising way of anticancer therapy without damaging healthy tissues. Hence, search for new molecular targets capable of specific apoptosis triggering is highly challenging, mainly due to new and effective anti-cancer therapies development. Cancer cell metabolism (1) and mainly mitochondrial metabolism (2) seems to represent intriguing target. Mitochondria play essential role in life of the cell but on the other hand mitochondria activate cell death which is usually connected to increased ROS (reactive oxygen species) production. Contribution of this work is identification of vitamin E analogues as inhibitors of complex II and potent inductors of mitochondrial ROS in cancer cells. Additionally, we have demonstrated activation of conserved Hippo/Mst1 kinase in response to the vitamin E analogues which results in FoxO1 nuclear localization, NOXA gene transactivation, Bak protein activation, mitochondrial membrane permeabilisation and apoptosis induction. Next we searched for membrane proteins with increased expression in cancer cells exposed to iron deprivation. These proteins can play a role in iron metabolism of cancer...
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Vegetation of fir- and calcicolous beech forests of the Czech Republic
Boublík, Karel ; Sádlo, Jiří (advisor) ; Roleček, Jan (referee) ; Ewald, Jörg (referee) ; Walentowski, Helge (referee)
Boublík K. (2009): Vegetation of fir- and calcicolous beech forests of the Czech Republic. - Ms., 104 p. [PhD thesis; depon. in: Library of the Department of Botany, Faculty of Sciences, Charles University, Prague] The thesis proposes Braun-Blanquet phytosociological classification of calcicolous beech forests and fir forests of the Czech Republic. Calcicolous forests dominated by Fagus sylvatica (Fagion, Cephalanthero-Fagenion) were defined with the help of two approaches: (i) selection on the basis of correspondence of species composition with an expert delimited species group and (ii) the formalized and supervised Cocktail method. In the case of the Cocktail method, the definitions of associations were created by combinations of sociological species groups using logical operators. Dominance of single species was included in the definitions of associations. Only one association (Cephalanthero-Fagetum) was distinguished. This association usually occurs on limestone, calcareous sandstone and calcareous sandy marlite. However, it can be found also on basalt and phonolite. TWINSPAN analysis distinguished three vegetation types within Czech calcicolous beech forests which are interpreted as subassociations of Cephalanthero- Fagetum: (i) Cephalanthero-Fagetum seslerietosum caeruleae occurring on...
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The role of calcium influx and calcium sensitization in contraction of isolated arteries of normotensive and hypertensive rat
Bencze, Michal ; Zicha, Josef (advisor) ; Neckář, Jan (referee) ; Chalupský, Karel (referee)
Vascular resistance is mainly determined by the contraction of vascular smooth muscle (VSM), which is regulated by the phosphorylation of myosin light chain (MLC). VSM contraction is initiated by calcium influx into the VSM cells, which is mediated by transient receptor potential (TRP) channels and L-type voltage- dependent calcium channels (L-VDCC). On the other hand, calcium sensitization is a mechanism enhancing vascular contractile response at a given level of intracellular calcium by RhoA/Rho kinase pathway-mediated inhibition of myosin light chain phosphatase. In this thesis I present the data about i) the role of TRP channels in the mechanisms of vascular smooth muscle contraction, ii) enhanced contractility of arteries from spontaneously hypertensive rats (SHR), and iii) the differences in contraction of arteries from normotensive and hypertensive rats related to the role of RhoA/Rho kinase pathway in three types of experimental hypertension (SHR, Ren-2 transgenic rats and salt-sensitive Dahl rats). In the study concerning TRP channels, I compared the effects of three commonly used non-selective TRP channels inhibitors (2-APB, SKF-96365, FFA) on isolated arteries. Among them 2-APB was the most interesting because the observed inhibitory effects of 2-APB were dependent on the type of...
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Hypoglycaemia as Acute Complication of Diabetes Mellitus
Krnáčová, Veronika ; Macek, Karel (advisor) ; Rybka, Jaroslav (referee) ; Švihovec, Jan (referee)
Title of thesis: Hypoglycaemia as acute complication of diabetes mellitus Author of thesis: Mgr. Veronika Krnáčová (roz. Vlčková) This thesis was focused on hypoglycaemia as adverse drug reaction of rosiglitazone, pioglitazone, nateglinide and repaglinide, which have been launched to the UK market and on severe hypoglycaemia (SH) requiring the assistance of Emergency medical service (EMS) in region of Hradec Králové. Observed factors were described in two separate sections. Hypoglycaemia as adverse drug reaction of selected oral antidiabetics Aim The aim of this study was to quantify the incidence of hypoglycaemic events during the first nine months of treatment with newly launched oral antidiabetics, rosiglitazone, pioglitazone, nateglinide or repaglinide, prescribed by general practitioners in England, to observe pattern of these events over time, to look for patients' characteristics which could influence the incidence of hypoglycaemia and to analyse the relationship between use of concomitant anti-diabetic therapies and the incidence of hypoglycaemia in patients treated with pioglitazone. Methods We used data collected for prescription-event monitoring (PEM) studies of rosiglitazone, pioglitazone, nateglinide and repaglinide. PEM is an observational, non-interventional, cohort study. Incidence...
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Preparation of precursors to ferrocenophane ligands
Škoch, Karel ; Štěpnička, Petr (advisor) ; Veselý, Jan (referee)
Title: Preparation of precursors to ferrocenophane ligands Autor. Karel Škoch Department: Department of Inorganic Chemistry (Faculty of Science, Charles University in Prague) Supervisor: doc. RNDr. Petr Štěpnička, Ph.D. This thesis focuses on a comparison of two alternative literature methods for the preparation [3]ferrocenophan-1-one. One-step Friedel-Crafts acylation of ferrocene with acryloylchloride in presence of aluminium chloride doesn't provide satisfactory yields and the product is contamined with inseparable impurities. For the preparation of a larger amount of pure [3]ferrocenophan-1-one is therefore suggested the original three-step synthesis starting from ferrocenecarbaldehyde. Ferrocenecarbaldehyde is first reacted with malonic acid to give β- ferrocenylacrylic acid, which can be easily reduced with hydrogen to β-ferrocenylpropionic acid. Treatment of β-ferrocenylpropionic acid with trifluoracetic anhydride acid induces heteroannular cyclization, affording [3]ferrocenophan-1-one with good purity and yields (up to 70 % over the three steps). All compounds were characterised by spectroscopic methods (1 H NMR, 13 C NMR and IR).
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Text authorship classification with unknown authors
Dolník, Karel ; Hajič, Jan (advisor) ; Mírovský, Jiří (referee)
Statistical and computational authorship attribution is a widely researched topic in literary science, but few works deal with solving the problem when the classified text does not belong to any of the authors the model saw during training. This work seeks a way to detect such an unknown author using machine learning methods commonly used for authorship attribution, especially the SVM classifier. He we introduce a modified One-versus-Rest-and-None classification scheme, which extends the One-versus-Rest scheme by training with data that does not belong to any classified author. This can be done using synthetically produced data or data from authors who are certain to have no connection to the classified texts. It turned out that the smallest decrease in accuracy occurred when synthetic data is used, compared to the classification without detection of an unknown author.
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