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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
In Vitro Selection of Aptamers for Methionine Sulfoxide
 Jureček, Matěj ; Míšek, Jiří (advisor) ; Bařinka, Cyril (referee)
Oxidation of methionine to methionine sulfoxide in proteins is considered one of important post-translational modifications of proteins. This modification can activate and also inhibit functions of many proteins and it is a part of regulation mechanisms of various (patho)physiological processes. For further research of the effects of methionine oxidation in proteins it would be very helpful to find its bioindicator. So far however, there has not been found any such antibody, nor any of its alternatives. This thesis was concerned with the search of ssDNA aptamer specific for methionine sulfoxide by the method of in vitro selection (SELEX). Several conditions for in vitro selection of methionine sulfoxide were tested in this diploma thesis. None of them led to the enrichment of the starting oligonucleotide pool and no selective aptamer for methionine sulfoxide has been found. Such results don't necessarily point to the impossibility of finding such aptamer, but the conventional methods used in this thesis weren't suitable for this task. In a control in vitro selection there has been found an enriched ssDNA pool for sulforhodamine B as a ligand. Sequencing of clones of this enriched pool has shown oligonucleotides with G-rich sequences, which is typical for already published aptamers for sulforhodamine B.
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Riboswitches as tools for gene expression regulation
 Jureček, Matěj ; Folk, Petr (advisor) ; Krásný, Libor (referee)
Riboswitches are segments of pre-mRNAs and mRNAs, present in their UTRs or introns, which are able to bindsmall molecules (usually a metabolite, ion or nucleotide) and in response "switch" between two conformations, which can affect the downstream gene expression process. In most cases, at least in bacteria, the mRNA which the riboswitch regulates encodes a component of the metabolism of the riboswitch-binding ligand. Contrary to other mechanisms of gene expression regulation, riboswitches do not require the participation of a protein. They may represent a way for the hypothetical early forms of life to regulate their genes. Riboswitches are abundant in bacteria and there is only a handful in eukaryotes. The scope of the study of riboswitches is ever more increasing and today it could very well be an independent branch of molecular biology.
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5 Jureček, Martin
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