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Mites and allergy.
Hrabcová, Veronika ; Jílek, Petr (advisor) ; Kudláčková, Zděnka (referee)
Veronika Hrabcová Allergy and house dust mites. Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Healthcare bioanalytics - Medical Laboratory Technician Background: Based on the literature we attempted to clarify the effect of moisture and temperature on the population of mites in dwellings. What are the mechanisms that reduce house dust mites and whether in the past fluctuating temperature and blankets freezing influenced the incidence of allergy. Main findings: The population of mites affecting many different factors, the most important are temperature and humidity. Mites D. pteronyssinus preferred the conditions of 23 ř C and 75% relative humidity (RH). During the winter, we can commonly found in homes average humidity around 25-45% RH. Through the day, mites are exposed to the fluctuating humidity, which are relatively well adapted. In combination with temperature, it is possible to create an unfavorable environment for their development and survival. When temperature increases, RH decreases below critical threshold humidity (CEH - Critical equilibrium humidity) and the mites are unable to obtain water from the ambient environment and thus complement their losses due to evaporation. Similar conditions apply as well in the air mattresses. On these principles...
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Opinions, knowledge, perception of risks and compliance of patients using non-prescription drugs and dietary supplements II.
Hrabcová, Veronika ; Malý, Josef (advisor) ; Doseděl, Martin (referee)
1 ABSTRACT Opinions, knowledge, perception of risks and compliance of patients using non- prescription drugs and dietary supplements II. Author: Veronika Hrabcová1 Tutor: Josef Malý1 1 Department of Social and Clinical Pharmacy, Charles University in Pague, Faculty of Pharmacy in Hradec Králové Introduction: In recent years, the self-medication in the Czech Republic has become an important part of health care of citizens who are actively involved in this process. OTC drugs and dietary supplements are an integral part of it. With their increasing supply in pharmacies, the cooperation of patients and professionals in pharmacies has become more important. Objectives: The aim of the thesis was to investigate the current opinions, attitudes, awareness and knowledge of the Czech patients who come into contact with OTC drugs. Methods: The data were collected from September 2013 to February 2014. The directed interview was conducted according to a prepared questionnaire.The data were collected in two surgeries of general practitioners for adults in the town of Uherské Hradiště. The obtained data were transferred to an electronic form using Google and subsequently processed using Microsoft Excel 2010. In this program, the data were evaluated by means of the frequency analysis and converted into tables and graphs,...
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Opinions, knowledge, perception of risks and compliance of patients using non-prescription drugs and dietary supplements II.
Hrabcová, Veronika ; Malý, Josef (advisor) ; Doseděl, Martin (referee)
1 ABSTRACT Opinions, knowledge, perception of risks and compliance of patients using non- prescription drugs and dietary supplements II. Author: Veronika Hrabcová1 Tutor: Josef Malý1 1 Department of Social and Clinical Pharmacy, Charles University in Pague, Faculty of Pharmacy in Hradec Králové Introduction: In recent years, the self-medication in the Czech Republic has become an important part of health care of citizens who are actively involved in this process. OTC drugs and dietary supplements are an integral part of it. With their increasing supply in pharmacies, the cooperation of patients and professionals in pharmacies has become more important. Objectives: The aim of the thesis was to investigate the current opinions, attitudes, awareness and knowledge of the Czech patients who come into contact with OTC drugs. Methods: The data were collected from September 2013 to February 2014. The directed interview was conducted according to a prepared questionnaire.The data were collected in two surgeries of general practitioners for adults in the town of Uherské Hradiště. The obtained data were transferred to an electronic form using Google and subsequently processed using Microsoft Excel 2010. In this program, the data were evaluated by means of the frequency analysis and converted into tables and graphs,...
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BRAF mutations in metastatic malignant melanoma.
Hrabcová, Veronika ; Hronek, Miloslav (advisor) ; Vrbacký, Filip (referee)
Bc.Veronika Hrabcová BRAF mutations in metastatic malignant melanoma. Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Healthcare bioanalytics - Specialist in Laboratory Methods Backround: Melanoma is malignant disease with increasing incidency. Treatment of advanced stage of melanoma is still limited. With a progress of knowledge in genetics and tumorigenesis, the incidence of mutated BRAF protein was observed at 50 % of melanomas. In 80-90 % mutated melanomas contain BRAF V600E mutation. The aim of study was to establish a suitable molecular biological method for the diagnosis of mutations in codon V600 BRAF. Methods: Cobas 4800 BRAF V600 mutation test and BRAF StripAssay test were used to analyze DNA. Cobas 4800 BRAF V600 mutation test is based on PCR using TaqMan probes designed for the wild-type and mutant BRAF V600E sequence. BRAF StripAssay test is based on PCR amplification with biotinylated primers and subsequent hybridization of the stripped with allele-specific oligonucleotide probes. Examined DNA samples were derived from 35 patients with advanced malignant melanoma or from archive of laboratory. Results: BRAF V600 mutation was detected in approximately half of the tumors, consistent with the results of other studies. In comparison methods Cobas test...
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Mites and allergy.
Hrabcová, Veronika ; Jílek, Petr (advisor) ; Kudláčková, Zděnka (referee)
Veronika Hrabcová Allergy and house dust mites. Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Healthcare bioanalytics - Medical Laboratory Technician Background: Based on the literature we attempted to clarify the effect of moisture and temperature on the population of mites in dwellings. What are the mechanisms that reduce house dust mites and whether in the past fluctuating temperature and blankets freezing influenced the incidence of allergy. Main findings: The population of mites affecting many different factors, the most important are temperature and humidity. Mites D. pteronyssinus preferred the conditions of 23 ř C and 75% relative humidity (RH). During the winter, we can commonly found in homes average humidity around 25-45% RH. Through the day, mites are exposed to the fluctuating humidity, which are relatively well adapted. In combination with temperature, it is possible to create an unfavorable environment for their development and survival. When temperature increases, RH decreases below critical threshold humidity (CEH - Critical equilibrium humidity) and the mites are unable to obtain water from the ambient environment and thus complement their losses due to evaporation. Similar conditions apply as well in the air mattresses. On these principles...
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