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Analysis of the Selected Company by the Selective Methods
Lucký, Jakub ; Herzogová, Eva (referee) ; Hanušová, Helena (advisor)
The objective of this thesis is to analyze the Wine wellness center in Hustopeče. The work is divided into the several parts. The introductory part describes the theoretical background, followed by analysis of selected subject using PESTLE analysis, Porter's five-factor model, marketing mix and SWOT analysis. On the basis of these analyzes is proposed recommendations and suggestions.
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TaqMan-based nucleic acid quantification - abilities and limits with regards to type of collection, age and quality of human specimen
Herzogová, Eva ; Daňková, Pavlína (advisor) ; Kološtová, Katarína (referee)
Real-time PCR method is a type of PCR which allows continual monitoring of DNA amplification during every cycle of its process. It is mostly used for gene expression analysis. Based on the results of previous experiments, we decided to test out the effect of anticoagulants EDTA, heparin, sodium citrate and CPDA on the expression of selected genes of the immunological spectrum and further, to test how the time period between drawing the blood and processing of blood sample influences mRNA levels of selected genes that are determined by changes in gene expression and/or mRNA degradation. To quantify mRNA of the studied genes, we isolated total RNA from the peripheral blood leucocytes and transcribed it into cDNA by using the reverse transcription PCR. This cDNA served as a template for the real-time PCR. To examine the changes of the expression caused by the effect of each particular anticoagulants, peripheral blood derived from 10 volunteers was used (each donor's blood was taken into 3 vacuum tubes with EDTA, heparin and sodium citrate anticoagulant agents). Next to that, we obtained 10 buffy coat samples in transfusion blood bags with CPDA anticoagulant agent. Compared to blood cells influenced by one of the three anticoagulant agents present in vacuum tubes, cells from transfusion bags affected...
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Analysis of the Selected Company by the Selective Methods
Lucký, Jakub ; Herzogová, Eva (referee) ; Hanušová, Helena (advisor)
The objective of this thesis is to analyze the Wine wellness center in Hustopeče. The work is divided into the several parts. The introductory part describes the theoretical background, followed by analysis of selected subject using PESTLE analysis, Porter's five-factor model, marketing mix and SWOT analysis. On the basis of these analyzes is proposed recommendations and suggestions.
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