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Application of liquid chromatography for determination of selected amino-acids and agmatine in biological material
Hazuková, Niké ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Niké Hazuková Supervisor: Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of diploma thesis: High performance liquid chromatogramy determination of selected amino acids and agamtine in biological material Keywords: agmatine, L-arginine, L-citruline, L-ornitine, UHPLC, chronic wounds This thesis follows up the metod development for the determination of agmatine, L- arginine, L-citrulline, L-ornithine in chronic wounds using ultra-high performance liquid chromatography. The aim of this thesis was to optimize suitable chromatographic conditions for the already mentioned analytes with possibility to use this method in clinical research and practice. Four chromatographic columns were tested. Finally the Meteoric Core C18 BIO the dimensions 100 x 4.6 mm, 16 nm, 2.7 μm (YMC, Germany) was chosen, using these conditions: the mobile phase (MF) was 100% 10 mM AMFO (pH 2.5), the temperature was set at 25 řC, the MF flow rate was 0.25 mL / min, and the injection volume was 2 μL. After optimization of the separation conditions, the method was applied to biological material (exudate from chronic wounds), samples were modified by selected extraction procedures as ultrafiltration and protein precipitation....
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Diagnostics of type II diabetes mellitus
Hazuková, Niké ; Nachtigal, Petr (advisor) ; Kudláčková, Zděnka (referee)
Our bachelor thesis is focused on problematic issues of Type 2 diabetes mellitus. This kind of disease is increasing in today's population, with the presence of increasing obesity, it greatly affects patient with diabetes disease. The aim of this bachelor thesis is to describe pathogenesis of Type 2 diabetes mellitus, which is contributed to the metabolic syndrome and insulin resistance. We also describe the acute complications of the disease (e.g. hyperglycaemic hyperosmolar coma, hypoglycaemia) and then consecutive complications (e.g. diabetic nephropathy, diabetic retinopathy). The thesis is also focused on diagnostic laboratory methods of Type 2 diabetes mellitus (determines e.g. venous plasma glucose, glycated haemoglobin, C-peptide), which are essential factors for early detection of metabolism disorders of sugars and fats and which could be potential indicators for the development of diabetes mellitus and could provide early dietary and therapeutic treatment.
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