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Development of HPLC-MS/MS methods for selected, biologically active compounds
Hrabáková, Kateřina ; Bosáková, Zuzana (advisor) ; Tůma, Petr (referee)
This thesis is focused on the development of an UHPLC-MS/MS method for the determination of lactone and carboxylate form of topotecan in aqueous buffers (Part 1) and on the development of a HPLC-MS/MS method for monitoring the extraction efficiency of 6 organic UV filters from aqueous matrices using deep eutectic solvents and terpenes (Part 2). Topotecan is an important anticancer drug that inhibits topoisomerase I, but only its lactone form is pharmacologically active. In the first part of this work, the change in the ratio of lactone and carboxylate form of topotecan in time was studied as a function of sample pH and the pH of the aqueous component of the mobile phase. For this purpose, a 19-minute UHPLC-MS/MS method with a C18 stationary phase, gradient elution and electrospray ionization in positive mode was developed. It was observed that with an increasing pH of the mobile phase and the pH of the sample, the mass fraction of the carboxylate form of topotecan in the sample increased as well. The same trend was also observed with increasing time since sample preparation. If methanol was added to the sample, a stabilization of the lactone form and a slower transition to the carboxylate form was observed. These findings can be further exploited to optimize the use of topotecan in medicine. The...
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Verbal Periphrases Expressing the Durativity of Verbal Action
HRABÁKOVÁ, Kateřina
This bachelor's dissertation deals with durative verbal periphrases. The theoretical part is focused on their description and application from the perspective of Czech and Spanish grammars. The practical part confirms and alternatively disproves the knowledge gained in the theoretical part. It further deals with the translation of periphrases and examines their frequency in particular verb tenses and modes. The aim of the dissertation is to demonstrate how the Czech translators of Spanish fiction contained in parallel corpus Intercorp v9 deal with the issues of durative verbal periphrasis. Periphrasis can have more meanings and there are many ways to translate them.
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Marriage and divorce rates in the Czech Republic and the importance of marriage in the concept of Czech public
Hrabáková, Kateřina ; Procházková, Radka (advisor) ; Jana, Jana (referee)
The thesis is focused on the analysis of the demoraphic indicators marriage and divorce rate in years 2000 to 2014. Then the analysis is focused on qualitative characteristics in connection with the problem.
The first half of the work explains the theoretical concepts and metodological part. The second part is then focused on the actual analysis of selected indicators and the analysis of the qualitative characteristics. The analysis shows that the nuptiality and divorce rate have declaining trend of development. In case of nuptiality the lowest values in Czech republic were recorded in 2013, most marriages were then concluded in 2000. The divorce rate recorded the highest decline in 2012, while the most marriages were divorced in 2004. Qualitative analysis shows that the persons who want to get married the most in the future are single persons. The most negative responses were recorded for persons widowed and divorced. The most common reason for marriage is starting a family and children.
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