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Nanofibre membranes as drug carriers 12
Fojtíková, Veronika ; Doležal, Pavel (advisor) ; Dittrich, Milan (referee)
The main advantages of sublingual systemic administration of drugs can be linked with a rich blood supply and very small thickness of the non-cornified biological sublingual epithelium. The theoretical part partially corrects the fact that the hitherto little addressed issue of sublingual administration route is connected with the influence of saliva and mucosal coating. The goal of the experiment was to verify the possibility of using albumin (BSA) as a part of potential drug carriers in the nanofiber membranes for sublingual administration. Sublingual membranes were obtained by dissection of porcine tongue (Sus scrofa) followed by rapid freezing in liquid nitrogen and further storage at -20 ř C. In vitro experiments involved the use of saliva as a medium of intermediate contact of drugs with the sublingual mucosa epithelium. Permeation of fluorescently labelled bovine serum albumin (FITC-BSA molecular weight of about 68. 000) was evaluated. Its application in the form 32-layered and 16-layered nanofiber carriers with the content of 30 percent FITC- BSA by weight was compared to permeation of the solutions of equivalent concentrations of lyophilized FITC-BSA and yielded the following findings: 1. FITC-BSA penetrated sublingual porcine membrane always by the flux J in the order of 101 µg/cm2 . h-1...
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Nanofibre membranes as drug carriers 12
Fojtíková, Veronika ; Doležal, Pavel (advisor) ; Dittrich, Milan (referee)
The main advantages of sublingual systemic administration of drugs can be linked with a rich blood supply and very small thickness of the non-cornified biological sublingual epithelium. The theoretical part partially corrects the fact that the hitherto little addressed issue of sublingual administration route is connected with the influence of saliva and mucosal coating. The goal of the experiment was to verify the possibility of using albumin (BSA) as a part of potential drug carriers in the nanofiber membranes for sublingual administration. Sublingual membranes were obtained by dissection of porcine tongue (Sus scrofa) followed by rapid freezing in liquid nitrogen and further storage at -20 ř C. In vitro experiments involved the use of saliva as a medium of intermediate contact of drugs with the sublingual mucosa epithelium. Permeation of fluorescently labelled bovine serum albumin (FITC-BSA molecular weight of about 68. 000) was evaluated. Its application in the form 32-layered and 16-layered nanofiber carriers with the content of 30 percent FITC- BSA by weight was compared to permeation of the solutions of equivalent concentrations of lyophilized FITC-BSA and yielded the following findings: 1. FITC-BSA penetrated sublingual porcine membrane always by the flux J in the order of 101 µg/cm2 . h-1...
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The kinetic analysis of the enzyme reaction catalyzed by the globine coupled histidine kinase
Fojtíková, Veronika ; Martínková, Markéta (advisor) ; Vaněk, Ondřej (referee)
Two-component signal systems serve as basic stimulus-response coupling mechanism to allow organisms (predominantly bacteria) to sense and respond to changes in many environmental conditions. The prototypical system consists of two proteins, namely a histidine kinase, containing a sensor domain and catalytic kinase core, and a response regulator protein (RR protein). Extracellular stimuli are sensed by a histidine kinase sensor domain. Then ATP is bound to the catalytic kinase core and the γ-phosphoryl group is transferred to the conserved histidine residue. This phosphoryl group is subsequently transferred to a conserved aspartate residue within the RR protein. Phosphotransfer to the RR protein results in activation of a downstream effector domain that elicits the specific response (usually it is transcription activity, but a few RR proteins function as enzymes). The histidine kinase sensor domain is designed for specific ligand interactions. This master thesis focused on the unique histidine kinase containing a sensor domain with a globine structure, which coordinates a heme molecule, namely globin-coupled histidine kinase from Anaeromyxobacter sp. Fw 109-5 (AfGcHK) and its appropriate RR protein. The aim of this thesis was to study and characterize the phosphorylation activity of AfGcHK and RR...
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Important roles of heme as a signal and a gas-sensing site: heme-sensing and gas-sensing proteins
Fojtíková, Veronika ; Martínková, Markéta (advisor) ; Hudeček, Jiří (referee)
Heme-containing sensor proteins are heme proteins, which are divided into two groups: heme-sensing and gas-sensing proteins. The function of heme-sensing proteins is affected by heme availability. Association (or dissociation) of heme moiety of heme-sensing protein regulates various physiological functions, including protein kinase activity, transcription and other important functions essential for cell survival. In gas-sensing proteins, heme acts as the sensing site for binding of gaseous molecules (including O2, NO and CO) and indirectly regulates physiological functions, including protein kinase activity, transcription and other important functions essential for cell survival. The recent studies on heme-containing senzor proteins published in scientific journals are summarized in this thesis. The experimental part of this thesis focused on the specific heme-containing sensor protein - a globin-coupled histidine kinase from Anaeromyxobacter sp. strain Fw 109-5 (AfGcHK). The aim of this thesis was to amplified and isolate plasmid carrying gen for AfGcHK. Consequently the protein was expressed in E.coli BL-21(DE3) and the protein was isolated. Based on the results, the isolation process was optimized. Moreover, the purified preparation of isolated AfGcHK was prepared in more than 99% of homogeneity....
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View of existing supplements for tissue of brain nutrition.
Fojtíková, Veronika ; Zemanová, Jana (referee) ; Vránová, Dana (advisor)
This diploma thesis is related to usage of food supplements in general. It observes which food supplements are mostly used, how often and in which period they are used and also which supplements forms population prefer. Next is this diploma paper focused on supplements for the support of cerebration, concretely up to supplements containing Ginkgo biloba. It studies their vascular activity, effect on memory and concentricity in various age groups. Also HPLC method for quick analysis of selected food supplements containing Ginkgo biloba was tested.
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