|
| |
|
|
Supported Phospholipid Bilayers and their Interactions with Proteins Studied by Ellipsometry, Atomic Force Microscopy and Confocal Fluorescence Microscopy
Macháň, Radek ; Hof, Martin (advisor) ; Fidler, Vlastimil (referee) ; Konopásek, Ivo (referee)
Supported lipid bilayers have been used as an artificial model of biological membranes and their interaction with 5 selected antimicrobial peptides was studied by several experimental techniques, mainly ellipsometry, laser scanning microscopy and fluorescence correlation spectroscopy. The thesis explains basic principles of the applied techniques focusing on their aspects relevant to characterization of lipid bilayers. The biological significance of antimicrobial peptides, their modes of interaction with membranes and the basic characteristics of the selected peptides are briefly discussed. The following text describes the main types of experimental studies performed and the interpretation of their results. Peptide-induced changes in lipid bilayer morphology were characterized by ellipsometry and laser scanning microscopy. Most interesting effects were observed in the case of melittin, which induced formation of long lipid tubules protruding from the bilayer. Lipid lateral diffusion measured by fluorescence correlation spectroscopy can provide information on bilayer organization on length-scales below resolution of optical microscopy.
|
|
| |
|
|
Efficiency and private financing in public higher education sector of the Czech Republic
Fidler, Vojtěch ; Pertold-Gebicka, Barbara (advisor) ; Víšek, Jan Ámos (referee)
Public higher education in the Czech Republic is financed by general taxation. Due to permanent lack of financial sources in the sector, there should be sought alternative ways of financing, among which private financing is considered to be the best alternative. Nevertheless, it is important that these additional funds are managed efficiently. Thus, next to private financing, the thesis also studies efficiency of the sector as these two areas are interrelated. The results of private funding model indicate feasible realization with relatively low negative impact on private rate of return. Using DEA method, the efficiency analysis reveals that although Czech universities are teaching efficiently, they lack efficiency in research. This suggests that current financing scheme should be adjusted accordingly. Higher education is also studied from theoretical perspective and discussed are such topics as quality, mass education or the recent reform proposal.
|
|
|
Risk appetite estimation on financial markets
Fidler, Vojtěch ; Geršl, Adam (advisor) ; Babin, Adrian (referee)
The thesis studies role of risk appetite on financial markets. In theoretical part, author describes a notion of this concept, refers to known methods and describes the role of behavioral economics in treatment of this concept. In practical part, models are constructed to explain influence of selected indices on CDS which proxy for sovereign risk of individual developed and emerging markets. Across the globe, there is found strong common component which can be explained by selected indices. It is also observed that GRAI indicator can play role in case of emerging markets. In case of developed markets, however, this property is missing. Granger causality does not prove relationship of GRAI explanation power in direction to sovereign risk.
|
|
|
Supported Phospholipid Bilayers and their Interactions with Proteins Studied by Ellipsometry, Atomic Force Microscopy and Confocal Fluorescence Microscopy
Macháň, Radek ; Hof, Martin (advisor) ; Fidler, Vlastimil (referee) ; Konopásek, Ivo (referee)
Supported lipid bilayers have been used as an artificial model of biological membranes and their interaction with 5 selected antimicrobial peptides was studied by several experimental techniques, mainly ellipsometry, laser scanning microscopy and fluorescence correlation spectroscopy. The thesis explains basic principles of the applied techniques focusing on their aspects relevant to characterization of lipid bilayers. The biological significance of antimicrobial peptides, their modes of interaction with membranes and the basic characteristics of the selected peptides are briefly discussed. The following text describes the main types of experimental studies performed and the interpretation of their results. Peptide-induced changes in lipid bilayer morphology were characterized by ellipsometry and laser scanning microscopy. Most interesting effects were observed in the case of melittin, which induced formation of long lipid tubules protruding from the bilayer. Lipid lateral diffusion measured by fluorescence correlation spectroscopy can provide information on bilayer organization on length-scales below resolution of optical microscopy.
|
|
|
Study of light-harvesting antennae based on bacteriochlorophyll aggregates
Alster, Jan ; Pšenčík, Jakub (advisor) ; Fidler, Vlastimil (referee) ; Balaban, Teodor Silviu (referee)
Title: Study of light-harvesting antennae based on bacteriolorophyll aggregates Author: Jan Alster Department: Department of Chemical Physics and Optics Supervisor of the doctoral thesis: doc. RNDr. Jakub Pšenčík, Ph.D. Abstract: Artificial photosynthesis is a potential future source of renewable energy. e light-to-emical energy conversion process starts with capturing light. Chlorosomes of green phototropic bacteria are probably the most efficient light-harvesting antenna found in the Nature. Moreover, their unique structure based on a self-organised ag- gregate of pigment molecules makes them relatively easy to mimic in vitro. is work explores formation and properties of self-assembled aggregates of bacteriolorophyll molecules in aqueous solvents by means of steady state and time resolved optical spec- troscopy with time resolution in the microsecond to femtosecond range. Various ag- gregation inducing agents have been tested. Isoprenoid quinones introduce a redox- dependent excitation energy quening meanism into the bacteriolorophyll aggre- gates. Carotenoids enhance the light-harvesting properties of the aggregates by cap- turing light in the spectral region where bacteriolorophyll does not and transferring the excitation energy to bacteriolorophyll. e results indicate that self-assembled...
|
|
| |
|
| |
|
| |
guest ::
RSS feed.