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Role of antioxidant defense in the synthesis of antidiabetic lipokines
Domanská, Veronika ; Kuda, Ondřej (advisor) ; Zouhar, Petr (referee) ; Vrkoslav, Vladimír (referee)
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered group of lipokines consisting of a fatty acid attached to a hydroxy fatty acid with an ester bond resulting in a diverse group of compounds with many different biological activities. Antidiabetic and anti-inflammatory activities are the most studied. The thesis aimed to study this group of bioactive lipids to elucidate the metabolism of FAHFAs and describe the role of antioxidant defense, namely peroxiredoxin 6 (Prdx6), in their biosynthesis with the help of isotopic labeling together with in vitro and in vivo experiments. All the samples, including white adipose tissue, liver, and even human breast milk, were subjected to untargeted or targeted lipidomic and metabolomics analysis using LC-MS/MS. We used the data from isotopically labeled experiments to describe the role of 5-PAHSA in glucose uptake and to show which specific pathways are stimulated by 5-PAHSA administration and which by insulin to compare the effect of both antidiabetic agents. In our samples, we analyzed TAG estolides, which function as intracellular reservoirs of FAHFAs, and managed to describe the role of specific lipases in their metabolism. We reported the role of Prdx6 and the specific enzymatic activity involved in synthesizing precursors that could...
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Framing of Islam in the Czech News
Domanská, Veronika ; Nečas, Vlastimil (advisor) ; Jirák, Jan (referee)
Using a qualitative content analysis, the thesis "Framing of Islam in the Czech News" examines the framing of Islam and Muslims in the Czech daily newspapers Mladá fronta DNES and Právo before and after the terrorist attacks in Paris (7-8 January 2015). Its theoretical framework views Islam both from the perspective of Czech society and the Czech media, the study of the latter aspect drawing on Czech as well as foreign theoretical works and research focused on Islam in the media. The thesis also considers the concept of media framing. The qualitative analysis aims to identify a set of frames specific to the media construction of Islam and Muslims. The extent to which framing is influenced by location (Islam and Muslims in the Czech Republic and abroad) and the Paris events (the development of framing over time - before and after the attacks) is also explored. Finally, the research looks at the role played in writings on the subject by stereotypes, orientalism, new racism (namely islamophobia) and van Dijk's ideological square.
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Representation of Childhood in Advertising of Nonprofit Organizations
Domanská, Veronika ; Hejlová, Denisa (advisor) ; Shavit, Anna (referee)
The Bachelor's thesis entitled "Representation of Childhood in Advertising of Nonprofit Organizations" deals with the representation of children in advertisements, and in the advertising of nonprofit organizations in particular. The theoretical part defines childhood as a social construct, outlines the social perception of the construction of childhood from two contrasting perspectives; it puts the relationship between the child and the adult in historical context and highlights the symbolism and connotations associated with the child. The thesis provides a theoretical view on the use of childhood as a motif in both commercial and non- commercial advertising. The research part focuses on visual advertising messages of non-profit organizations that emerged in the Czech media (particularly in the press, television, outdoor advertising and on the Internet) between 2010 and 2012. Selected advertisements are subject to the qualitative visual analysis which aims to describe and compare the method of presenting the motif of childhood and its insertion within the emotional context.
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