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Electrooculogram based text comprehensibility evaluation
Netopil, Jan ; Čmiel, Vratislav (referee) ; Janoušek, Oto (advisor)
This thesis deals with principles and measuring of electrooculography (EOG) and with electrooculography signal utilization for assessment of reading comprehension. The basic physiology and anatomy of the eye, types of eye movement and identifiable elements in EOG during reading are described in thesis. Methodology of electrooculogram-based text comprehensibility evaluation is described and verified by recording of EOG of ten volunteers using Biopac system. Detection of identifiable elements is implemented by Matlab. The last steps of process are statistical evaluation of data and revers identification of text complexity.
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Reccurence plot for heart rate variability analysis
Franěk, Pavel ; Čmiel, Vratislav (referee) ; Janoušek, Oto (advisor)
The aim of this thesis is to describe the variability of cardiac rhythm and familiarity with the methods of the analysis, ie by monitoring changes in heart rhythm electrogram signal recording and using the methods in the time domain using recurrent diagram. The work describes the quantification of the methods and possibilities of quantifiers in the evaluation of heart rate variability analysis. It also describes the clinical significance of heart rate variability and diagnostic capabilities changes of heart rate variability caused by ischemic heart disease. The practical part describes how to create applications in Matlab to calculate the quantifiers analysis of heart rate variability in the time domain using recurrent diagram. The calculation was made of the positions R wave elektrogram signal isolated rabbit hearts. The calculated values of quantifiers both methods were statistically evaluated and discussed.
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Monitoring of mesenchymal stem cell migration in the extracellular matrix
Zumberg, Inna ; Čmiel, Vratislav (referee) ; Chmelíková, Larisa (advisor)
This master’s thesis contains a description of mesenchymal stem cells (MSC). The work includes knowledge about the process of migration and differentiation of MSCs. The theoretical part of this thesis also deals with the problem of cell cultivation in 2D and 3D environments. The most used materials for creating 3D scaffolds are described here. The practical part contains the description of the cell culture protocol for passaging and also describes the experiment in the cell laboratory. The results of the experiment are discussed using confocal microscope images. The proposed experiment was tested with a sufficient number of repetitions. The processing of microscopic images was performed in the MATLAB programming environment.
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Psychogalvanometric measurement and biofeedback
Paulasová, Lenka ; Čmiel, Vratislav (referee) ; Hrozek, Jan (advisor)
This work deals with bio-feedback and the possibility of measuring changes in skin resistance for biofeedback. The Theoretic part is focused on the principle of biofeedback and its use. Next deals with the resistance of the skin, the changes depending on the emotions and the measurement this change . The practical part is devoted to the creation program for the use of skin resistance to change biofeedback in LabVIEW.
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Membrane potential measurement with voltage sensitive dyes in fluorescence microscopy
Tkáč, Jan ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
The aim of this work is to make a literature search in the measurement of membrane voltage using voltage-sensitive dyes and suggest a method for measuring the membrane voltage on the available cells using the voltage-sensitive dye di 4 ANEPPS and its further implementation. The work contains an introduction to electrophysiology of cells, and explains typical fluorescence characteristics. The thesis contains the description of a fluorescence microscope. The document presents characteristics of voltage-sensitive dyes and their distribution. A large part of the work describes the implementation and measurement of the experiment. The document also includes different methods for measuring and processing of all results.
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Motion Analysis
Hudec, Jiří ; Čmiel, Vratislav (referee) ; Sekora, Jiří (advisor)
Bachelor thesis is dealing with how to analyze human walk with Vicon camera system. In the first chapter are described elementary ideas for gait analysis and principal methods of Vicon system. There is also discussed basic layout of cameras in the laboratory and layout of markers attached at a human body. The second chapter is also about synchronisation of multiple cameras and description of CVS format. Further, in the third chapter are discussed four algorithms created in Matlab for a description of gait analysis. In the last fourth chapter are discussed the results of the software from Matlab and comparison these results with results made in the laboratory of gait analysis.
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Cell Viability Measurement
Pelc, Pavel ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
This work discusses the viability of cells. It describes the principle of fluorescence, fluorescent methods, fluorescent microskopy and the method of dyeing of the cells with a special kit that contains calcein AM and ethidium homodimer dyes. Further it describes the program for analysis and evaluation of viability on two different cellular cultures.
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Dynamic drawing analysis
Labounková, Ivana ; Čmiel, Vratislav (referee) ; Janoušek, Oto (advisor)
This thesis is focused on the dynamic drawing analysis. It deals with tracing drawing tests that can be applied for the analysis. It also deals with method for temporal segmentation of emerging drawings and also vectorization of resulted segments of drawings.
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Function Generator with the Android Operating System
Neradilková, Aneta ; Čmiel, Vratislav (referee) ; Sekora, Jiří (advisor)
Function generators are equipments that generate a wide range of waveforms. This bachelor’s thesis introduces the function generator and Arduino platform. On the basis of familiarity with the function generator and the Arduino platform is designed proposal generator using Arduino kit and draft flowchart for generating four waveforms. This proposal is implemented and describes the different parts of the generator, hardware and software. The functionality of the generator is tested using an oscilloscope.
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Experimental depth of field determination of optical imaging systems
Kubáček, Svatopluk ; Čmiel, Vratislav (referee) ; Odstrčilík, Jan (advisor)
The aim of this work is to introduce the principle of scanning in optical microscopy. This thesis deals with problem of parameters of display process and their influence on the results and measurements of these parameters. In detail there is analyzed the depth of field of the optical system and there are established some possibilities of theoretical and experimental determination. Part of this thesis is the realization of the measurement work place and program providing experimental depth of field based on different measurement methods. The conclusion is devoted to the practical part, which has the task to monitor settings and optical systems and their impact on depth of field.
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