|
|
Investigation of acid-based properties of mono- and diaza[5]helicenes by non-aqueous capillary electrophoresis and quantum chemical calculations
Šolínová, Veronika ; Štěpánová, Sille ; Jančařík, Andrej ; Klívar, Jiří ; Šámal, Michal ; Stará, Irena G. ; Vacek Chocholoušová, Jana ; Vacek, Jaroslav ; Starý, Ivo ; Kašička, Václav
Non-aqueous capillary electrophoresis (NACE) using methanol (MeOH) as a solvent of the background electrolytes was used to determine the thermodynamic acidity (ionization) constants (pKa) of a series of monoaza[5]helicenes and diaza[5]helicenes protonated to the first degree in MeOH and water. Depending on the number and position of the nitrogen atoms in their molecules, the analyzed aza[5]helicenes were found to be weak to moderate bases with methanolic pKa,MeOH in the range 3.90-8.75 and with aqueous pKa,H2O in the range 3.53-8.28.
|
|
| |
|
|
Laboratory markers in the process of extracorporal elimination of lipoproteins in long-term treatment of severe familial hypercholoesterolemia
CHOCHOLOUŠOVÁ, Jana
LDL-apheresis is a method of extracorporeal LDL-cholesterol elimination, which is known to decrease inflammatory processes of atherogenesis. The study is divided into two parts. THE AIM of the first part was to evaluate LDL-apheresis procedures based on immunoadsorption in 9 patients, 5 men and 4 women, (median age was 57, range 19-61 years), with severe familial hyperlipidemia (period 2004-2007). Immunoadsorption was performed by means of Cobe Spectra (COBE BCT, USA), ADA or Adasorb (Medicap, Germany) and columns of LDL-Lipopak (Pocard Ltd., Russia). Before and immediately after the procedure, blood samples were taken and serum levels of lipoproteins and some other parameters were determined. The aim of the second part was to evaluate the effectiveness of rheopheresis method and compare it with LDL-apheresis. Procedures of rheopheresis were implemented in 4 patients, 3 men and 1 woman, median age was 51 (35 {--} 67) years. During this procedure Cobe-Spectra was used; plasma was than pumped into the secondary grade filtrs Evaflux. Before and immediately after the procedure, blood samples were taken and serum levels of lipoproteins and some other parameters were determined.
|
guest ::
RSS feed.