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Green chromatography method development for red dyes determination in soft drinks
Boháčová, Tereza ; Ramos, María Carolina Fernández (advisor) ; Nováková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Boháčová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D., Dr. Maria Carolina Fernandez Ramos Title of diploma thesis: Development of green chromatography method for red dyes determination in soft drinks Green chromatography HPLC method was used and validated for the simultaneous determination of water-soluble red dyes - Carmoisine E 122, Amaranth E 123, Ponceau 4R E 124, Erythrosine E 127, Red 2G E 128, Allura Red AC E 129. The method was applied for direct determination of Amaranth E 123 and Ponceau 4R E 124 in drinks Syrup black currant (Neli, a.s.), energy drink Kamikaze (Power Life Energy), sparkling wine Bestivo & spritzzante (not shown), Syrup Raspberry light (VESETA spol. s.r.o), and beer Skalák Malina (Pivovar Malý Rohozec). Analytical column Chromolith Performance CN 100 x 4.6 mm was used and the mobile phase contained water / 1% (v/v) acetic acid buffer with addition of amonia for pH adjustment to value 7.0. Successful separation was obtained for all the compounds using an optimized gradient elution within 7 min. Method development was performed at a temperature of 45řC and flow rate 2ml/min. The diode-array detector (DAD) was used to monitor the colorants at the...
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Outdoor Education: the Norwegian Approach
Boháčová, Tereza ; Kasíková, Hana (advisor) ; Valenta, Josef (referee)
Research shows that being in nature is important for children's health and psychosocial and physical development. Outdoor education can significantly improve the quality and meaningfulness of learning, contribute to the teachers' and pupils' well-being and help to build a positive attitude towards nature. However, learning outside the school building is rather an exception in the Czech Republic and has not yet received much attention here. On the contrary, Norway is specific in its attitude to outdoor recreation and outdoor education. The purpose of this paper is to present Norwegian approach to outdoor education in primary schools. The theoretical chapters describe the issue of outdoor education in general with a specific focus on the Czech tradition and discourse, the Norwegian education system and the local situation of outdoor education. This part is followed by a qualitative research carried out in the Bodø district in northern Norway. The main method of data acquisition were interviews with teachers from seven local primary schools. The research describes the teachers' concept of outdoor education and answers the question of where the sources of support for outdoor education in Norway are.
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Green chromatography method development for red dyes determination in soft drinks
Boháčová, Tereza ; Ramos, María Carolina Fernández (advisor) ; Nováková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Boháčová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D., Dr. Maria Carolina Fernandez Ramos Title of diploma thesis: Development of green chromatography method for red dyes determination in soft drinks Green chromatography HPLC method was used and validated for the simultaneous determination of water-soluble red dyes - Carmoisine E 122, Amaranth E 123, Ponceau 4R E 124, Erythrosine E 127, Red 2G E 128, Allura Red AC E 129. The method was applied for direct determination of Amaranth E 123 and Ponceau 4R E 124 in drinks Syrup black currant (Neli, a.s.), energy drink Kamikaze (Power Life Energy), sparkling wine Bestivo & spritzzante (not shown), Syrup Raspberry light (VESETA spol. s.r.o), and beer Skalák Malina (Pivovar Malý Rohozec). Analytical column Chromolith Performance CN 100 x 4.6 mm was used and the mobile phase contained water / 1% (v/v) acetic acid buffer with addition of amonia for pH adjustment to value 7.0. Successful separation was obtained for all the compounds using an optimized gradient elution within 7 min. Method development was performed at a temperature of 45řC and flow rate 2ml/min. The diode-array detector (DAD) was used to monitor the colorants at the...
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The quality of water sources in the Czech Republic
Boháčová, Tereza ; Petrtýl, Miloslav (advisor) ; Kyriánová, Iveta Angela (referee)
The following bachelor thesis on the subject of The quality of water sources in the Czech Republic is supposed to introduce its readers to both historical development and current state of surface water quality in the Czech Republic. The works methodology is creation of a literature review of available scientific literature and especially Reports on surface water quality in particular river basins. The first part briefly describes Czech hydrological network and basic water quality indicators. The second part deals with the development of surface water quality in particular river basins. Another part of the work describes legal measures concerning surface water protection.
In the last century, surface water contamination was considered one of the biggest environmental problems in our country. Waste water flowing into surface water were not perfectly treated and often werent treated at all. Because of this and also because of intense industry development, surface water was highly polluted. It wasnt until 1989, when the surface water quality improved significantly, mainly due to reduction of contaminants discharge, waste water treatment plants and sewerage system construction and development. Main danger for quality of rivers after 1989 were floods in 1997 and 2002, during which water treatment plants outage were often and dangerous chemicals were leaking from factories.
The main finding of this work is the fact that quality of surface water in Czech Republic is much better than in the last century. However, it is still needed, that we focus on smaller rivers which are being endangered the most by discharge of contaminants from villages that dont have their own waste water treatment plants.
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