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Molecular mechanism of 14-3-3 protein dependent regulation of caspase-2
Kalábová, Dana ; Obšilová, Veronika (advisor) ; Pavlíček, Jiří (referee) ; Žáková, Lenka (referee)
Molecular mechanism of 14-3-3 protein dependent regulation of caspase-2 Abstract Caspase-2 is a protease standing apically in the cascade of reactions leading to apoptosis. Properly functional apoptosis eliminates damaged cells, autoreactive lymphocytes or redundant groups of cells in ontogeny. The process of caspase-2 activation must be precisely regulated. One of the described ways of caspase-2 regulation causing its inhibition is posttranslational modification phosphorylation with subsequent binding of the regulatory scaffold protein 14-3-3. The aim of this dissertation is to explain the molecular mechanism of this regulation. To understand the interaction between the proteins, it was necessary to first identify the phosphorylation sites in the caspase-2 molecule recognized by the 14-3-3 protein and then describe the detailed structure of the binding complex. The structure was characterized by a number of biochemical and biophysical methods, such as analytical ultracentrifugation, native electrophoresis in TBE buffer, polarization-fluorescence assay, hydrogen/deuterium exchange coupled to mass spectrometry, or crystallization; and the results led to stimulating conclusions. Activation of caspase-2 begins with its binding to adaptor proteins, cleavage and dimerization of the catalytic subunits. The...
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Effect of trunk stabilization on throwing velocity in heptathlon and decathlon athletes
Hanuliaková, Júlia ; Žáková, Lenka (advisor) ; Stupková, Michaela (referee)
Title: Effect of trunk stabilization on throwing velocity in heptathlon and decathlon athletes Background: Throwing disciplines are an important part of combined track and field events. A stable trunk is a necessary component of the kinematic chain in the generation of force during throwing. Throwing is usually ineffective if the leg and trunk muscles are unable to generate sufficient force or if there is no transfer of energy to the throwing arm. This paper examines the effect of trunk stabilization on throwing velocity. Objective: The aim of the study was to investigate whether it is possible to increase the throwing velocity of heptathlon and decathlon athletes by using exercises aimed at improving trunk stabilization. Methods: Measurement of throwing velocity using sports radar, assessment of trunk stabilization level using Janda's stereotype of push-up, Kolar's bear test and deep squat test and McGillʹs torso muscular endurance test battery. Results: During the ten-week intervention focused on trunk stabilization, the experimental group experienced an average improvement in throwing velocity of 5.76 km/h (8.9%). The control group, continuing with standard training, experienced only a slight improvement in throwing velocity of 1 km/h (2%). In the evaluation of trunk stabilization, the...
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Comparison of shoulder joint rotation ranges in relation to anthropometric measures in baseball and softball pitchers
Jelínková, Kateřina ; Žáková, Lenka (advisor) ; Vomáčková, Helena (referee)
Title: Comparison of the shoulder joint rotation in relation to anthropometric measures in baseball and softball pitchers Objectives: The main objective of this study was to compare the ranges of shoulder joint rotations in male baseball and softball pitchers due to differences in pitching biomechanics. Furthermore, to determine the difference in range of motion to rotations on the dominant and non-dominant upper extremity and to determine the prevalence of glenohumeral internal rotation deficits of the shoulder. The subject was also investigated to find possible relationships between measured anthropometric (HK length, arm span, biacromial width) values and goniometric values of range of motion into rotations including the prevalence of glenohumeral deficit. Methods: The research sample included 20 actives Czech Extraliga baseball and softball players, who were divided by sport for the purpose of the study. The baseball group included 10 probands (10 male, aged 25.1 ± 3.5 years, height 185.5 ± 7.8 cm, weight 93.7 ± 9.7 kg, years baseball of participation 14.9 ± 3.8 years). The softball group also included 10 probands (10 male, aged 23.1 ± 2.5 years, height 183.2 ± 8.1 cm, weight 88 ± 11.9 kg, years softball of participation 12.2 ± 1.8 years). None of the players reported an upper extremity injury...
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Comparison of upper and lower quadrant stability using the Y-balance test in girls practicing sports aerobics
Citová, Adéla ; Nováková, Tereza (advisor) ; Žáková, Lenka (referee)
Title: Comparison of upper and lower quadrant stability using the Y-balance test in girls practicing sports aerobics Aim: The aim of this thesis is to identify the functional development of girls practicing sports aerobics at the elite level, to compare asymmetries and dynamic stability of the lower and upper quadrants using the Y-balance test across age groups. In addition to the main aim, the thesis monitored the prevalence of musculoskeletal disorders using a short anamnestic questionnaire and assessed the presence of hypermobility through the Beighton score. So, a secondary aim is to determine if and how these other aspects affect the dynamic stability of the girls. Method: A total of 44 girls aged between 8 and 25 years, covering all age categories of sports aerobics, participated in the study. The probands were selected from nine clubs in the Czech Republic and the research took place in the gyms of six of them. Measurements were taken during the competition season between February 2023 and October 2023. Data were collected by anamnestic questionnaire, Y-balance test and Beighton score. During this time period, the girls were measured a total of 3 times - at the beginning of the season, at the midpoint and towards the end. Data were entered into forms and then processed in Microsoft Excel...
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Evaluation of dynamic stability of the lower limb in horse riders using the Y-balance test
Kamírová, Barbora ; Žáková, Lenka (advisor) ; Vomáčková, Helena (referee)
Title: Evaluation of dynamic stability of the lower limb in horse riders using the Y- balance test Objectives: The aim of this thesis is to map the level of lower limbs stability in show jumping and eventing horse riders by using the Y-balance test and to describe whether common signs of variations in range of motion of lower limb joints will be present. At the same time supplement the thesis with a short summary of movement problems and the use of physiotherapy in horse riders using a short questionnaire. By writing this thesis I would like to raise the awareness of riders about the effect of asymmetries and imbalances on the musculoskeletal system of both the rider and the horse, and about the possible achievement of better harmony between the rider and the horse when trying to correct these changes with the help of physiotherapy or other additional movement activities. Methods: The key part of the research section of the thesis is an examination using the Y-balance test evaluatimg the dynamic stability of the lower limb and also the examination of range of motion of the lower limb joints using a goniometer. At the same time, a questionnaire is part of the work, which has the task of providing a brief overview of the issue of physiotherapy in horse riding. Testing is performed on 25 probands, 14...
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Postural stability of the trunk in relation to lower limb injuries in floorball players
Balatka, Adam ; Nováková, Tereza (advisor) ; Žáková, Lenka (referee)
Author: Bc. Adam Balatka Title: Postural stability of the trunk in relation to floorball players' lower limbs injuries Objectives: The aim of this diploma thesis is to investigate the level of postural stability by evaluating the quality of the deep stabilization system function in elite floorball players. Then, take into account its relation to the occurrence of lower extremity injuries in the anamnesis of each player. The next goal is to compare these results with evaluation of the stabilizing abilities of the lower extremities. Methods: 28 elite floorball players from fhe FBC Liberec club took part in this research. The players were 16-35 years old. Testing and data collection were done in Semptember and October 2023 before practise unit in the gym of the floorball club FBC Liberec. Anamnestic data focused mainly on lower extremities injuries were taken at first from each player. Subsequently, the level of trunk stability was examined using the diaphragm test and the hip joint flexion test according to Kolář. Then was measured the Y-balance test. Finally was done the evaluation according to the Return to activity algorithm Level 4 (90ř balance hop and square hop test). The MS Excel 365 program and statistic software Jamovi (version 2.3) were used for data evaluation. The results were processed...
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Prevention of health risks among volleyball and beach volleyball players
Drahorádová, Olga ; Carboch, Jan (advisor) ; Žáková, Lenka (referee)
iv Abstract Title: Injuries in volleyball and beach volleyball and their prevention Objectives: The main goal of this diploma thesis is to compare the most common injuries in volleyball and beach volleyball in the Czech Republic and to find out their experience of subsequent recovery. Another goal is to find out what injury prevention athletes use and what experience they have with physiotherapy. Methods: Data were used from a non-standardized questionnaire which was filled out by Czech players of volleyball and beach volleyball. The obtained data were processed by software Microsoft Excel into tables and graphs. The questionnaire was completed by a total of 102 respondents. Results: It was evaluated that the highest frequency of injuries is found in the area of the ankle, knee joints and fingers in volleyball players. Conversely, beach players have a most injuries in the area of the lumbar spine and shoulder joints. They most often use kinesio tape of the compensatory aids and massage is the most popular from the forms of regeneration. Keywords: injuries, questionnaire, physiotherapy, Czech Republic
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A case report of physiotherapeutic care of a patient after double osteosynthesis of the humerus with postoperative paresis of the nerve radialis
Forst, Sabina ; Žáková, Lenka (advisor) ; Nováková, Tereza (referee)
Title: A case report of physiotherapeutic care of a patient after double osteosynthesis of the humerus with postoperative paresis of the nerve radialis Objectives: The aim of this thesis is the theoretical elaboration of knowledge concerning peripheral paresis with emphasis on the radial nerve, osteosynthesis of the humerus and the rehabilitation process. In a special part, the aim is to elaborate a case study of physiotherapeutic care, which focuses on postoperative paresis of the n. radialis after double osteosynthesis of the humerus, using both theoretical and practical knowledge from the bachelor's study. Methods: The thesis has two parts. In the theoretical part I have elaborated data on the shoulder joint, fractures, osteosynthesis of the humerus, peripheral nerves, paresis of the n. radialis and possibilities of rehabilitation in this diagnosis with the help of specialized sources. A special section is devoted to a complete case report of a patient with the given diagnosis. It includes patient's history, initial kinesiological analysis, 10 therapeutic units, output kinesiological examination and evaluation of the effect of therapy. Results: This thesis was prepared during the bachelor internship at Centrum léčby pohybového aparátu in Prague in January 2024. The therapies resulted in an...
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Case Study of Physiotherapeutic Care of a Patient after the osteosynthesis of Tibial Plateau Fracture
Fulín, Štěpán ; Žáková, Lenka (advisor) ; Těšitelová, Pavlína (referee)
Abstact Author Štěpán Fulín Thesis supervisor: PhDr. Lenka Žáková, Ph.D. Title: Case study of physiotherapeutic care of a patient after osteosynthesis of tibial plateau fracture. Objectives: The aim of this bachelor thesis is to elaborate a detailed case study of physiotherapeutic care of a patient diagnosed with osteosynthesis of tibial plateau fracture and to analyse theoretical knowledge from the literature concerning this diagnosis. Methods: This bachelor thesis is divided into two parts. The first part is theoretical and describes the anatomical and kinesiological context of the knee joint, and discusses the knowledge from the field of traumatology and rehabilitation of the specific diagnosis. The special part of the thesis is based on the professional practice that took place at the Centrum léčby pohybového aparátu Vysočany. This part contains a detailed case report of a patient with the mentioned diagnosis. The case report includes an initial and final kinesiological analysis, short-term and long-term physiotherapy plan, a record of the individual therapies and at the end is summarized the effect of the therapy. Results. It was succeded to increase the range of motion of the operated joint into flexion and to reduce pain at rest and during movement. Furthermore, it was possible to strengthen the...
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Case report of physiotherapeutic care of a patient with a diagnosis of knee replacement
Morávková, Tereza ; Žáková, Lenka (advisor) ; Sohrová, Jana (referee)
Author: Tereza Moravkova Supervisor: PhDr. Lenka Zakova, Ph.D. Title: Case report of physiotherapeutic care of a patient with a diagnosis of knee replacement Objectives: The aim of this bachelor thesis is to elaborate a case report of physiotherapeutic care of a patient diagnosed after knee TEP. At the same time, elaboration of the theoretical knowledge of the diagnosis and discussion of its treatment from the perspective of physiotherapy. Methods: I worked on the case report in the period 9.1. - 19.1.2024 at the workplace of CLPA s.r.o. In total, I completed 10 therapies with the patient, the first therapy being the initial kinesiological analysis. In the theoretical part, theoretical information concerning anatomy and kinesiology of the knee joint, osteoarthritis and its treatment, discussion of total knee replacements and the method of surgery are presented. Finally, a look at rehabilitation approaches after knee replacement surgery at the level of Evidence Based Medicine Results: The patient's physiotherapy care was uneventful. During the rehabilitation stay at CLPA, the patient's condition was positively affected. More detailed results are given in the conclusion of the examination and in the evaluation of the effect of the therapy. Keywords: knee joint, physiotherapy, osteoarthritis, total...
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