|
|
Aplikace age managementu v průmyslovém sektoru
Krejčová, Kateřina
Diploma thesis deals with an issue of age management and its introduction to the selected industrial companies. Literary research of the thesis defines general con-cepts related to human resources, specifications of older employees and their status on labour market and examines problematics of the age management in context of present demographic development in Czech Republic. In the thesis there is a survey amongst the employees of the selected companies. These surveys also contains an interviews with top management. In the final part of the thesis there are recommendations based on the findings which leads to the effective applica-tion of the age management in the industrial sector.
|
|
|
The role of FANCI phosphorylation in the Fanconi anemia DNA repair pathway
Krejčová, Kateřina ; Šilhán, Jan (advisor) ; Bárta, František (referee)
Fanconi anemia is an autosomal recessive disorder caused by mutation in one of Fanconi genes and it is manifested by developmental abnormalities, bone marrow failure, predisposition to cancer, cellular sensitivity to cross-linking agents and many other symptoms. Proteins encoded by Fanconi genes and some other proteins are part of Fanconi anemia pathway (FA pathway), which is responsible for DNA repair of an interstrand cross-link (ICL). The repair by this pathway requires monoubiquitination of FANCD2, which is induced and regulated by ATR dependent FANCI phosphorylation. The FANCI phosphorylation initiates the FA pathway but the molecular mechanism of this initialization is not known. Furthermore the proper function of entire pathway requires both: sequence of phosphorylation events of FANCI and monoubiquitination of FANCI:FANCD2 complex . The principle of this work was to study molecular mechanism of initiation and regulation of FA pathway by FANCI phosphorylation. Therefore phosphomimetic mutants of FANCI have been created to investigate their role in processes leading to FANCD2 monoubiquitination. The main aim was to reveal how the phosphorylation of FANCI affects DNA binding and also DNA binding of FANCI:FANCD2 complex. Since both DNA and FANCI phosphorylation are required for proper FANCD2...
|
|
|
Gargoyles of the European cathedrals, their origin, and their meaning
KREJČOVÁ, Kateřina
The bachelor thesis deals with the origin and meaning of gargoyles as well as traditional mythology, where it explores the sources of inspiration for their creation. The work presents examples of the European steel cathedral builders' works, by means of which they describe the way of creation and creation of gargoyles in the environment of the stoneworks. It also looks at the history of gargoyles in architecture, taking into account their symbolic meaning as part of the Gothic cathedral. The practical part refers to the theme of the work, which follows the aesthetics and function of the original gargoyles. The work is made in 1: 3 scale, using clay modeling and subsequent firing. The thesis contains complete photo documentation related to theoretical and practical parts and sketch material.
|
|
|
The God's Eye View: Epistemic Ideal or Conformity with Standards of Justification?
Krejčová, Kateřina ; Švec, Ondřej (advisor) ; Ritter, Martin (referee)
(in English) In the Enlightenment liberation from the religious myth one can see a paradoxical shift that is characterized by the fact that the scientist in the new type of secularized culture takes over the role previously dedicated to the priest. She holds the position of a mediator between what is human and superhuman, subjective, imperfect and flawed and a distant truth to which human beings feel a specific kind of responsibility. As the "soft sciences" ceded territory to the exact sciences, it happened because we have not yet been able to give up the dream of a perfectly neutral God eye's view. The author wants to show that objectivity itself is not impartial, but that it is a name for different kinds of rationality, which are at different times considered to be epistemic virtues.
|
|
|
Procedurally generated video games as a humanistic platform of the future
Salaba, Petr ; Miesenböck, Julia (advisor) ; Krejčová, Kateřina (referee)
An intermedia analysis of "art video games" in the perspective of humanistic values inspired by the theory of neorealism. Italian screenwriter and film theoretician Cesare Zavattini argues that a truly humanistic and socially conscious film would not be based on a dramatic plot, but that it would rather come from a factual depiction of every individual value inherent to the filmed topic. In my work, I propose that such a non-narrative approach can be well executed in the context on the new interactive media, concretely in video games that are based on procedurally generated environment rather than on a linear script.
|
|
|
Preparation of tumour ligand B7-H6 with coiled-coil tag and verifying of its binding to receptor NKp30
Krejčová, Kateřina ; Vaněk, Ondřej (advisor) ; Moserová, Michaela (referee)
Natural killer cells (NK cells) are part of innate antitumor immunity. These cells have the capacity to prevent viral infection or malignant transformation without prior antigen sensitization. Activation of NK cells consist of different recognition strategies. Mechanism of activation is based on down-regulated expression of MHC gp I molecules on the cell surface. NK cells possess both activation and inhibitory receptors that transmit activation or inhibitory signals which determine if NK cells are activated or not, and thus whether the target cell will be lysed or spared. NKp30 is a type I transmembrane receptor which recognize the stress-induced cell surface ligand B7-H6. Interaction of these two proteins leads to the initiation of immune response. The main aim of this thesis is the preparation of cell ligand B7-H6 with coiled-coil peptide tag in human embryonic kidney cell lines HEK293 GnTI- and HEK293T. Successful preparation of B7-H6 with coiled-coil tag on its C-terminus was verified by mass spectrometry. Its interaction with natural cytotoxicity receptor NKp30 was also proven by sedimentation analysis. Key words: NK cells, recombinant expression, B7-H6, HEK293, coiled-coil (This thesis is written in Czech)
|
|
|
Regimes of Rationality / Objectivity between Historical Epistemology and Pragmatism
Krejčová, Kateřina ; Švec, Ondřej (advisor) ; Ritter, Martin (referee)
(in English) The aim of the thesis is to introduce selected approaches to genealogy and changes of the concept of "objectivity". While historical epistemology puts this concept into a context of a particular historical period in which it was born and established itself, and therefore perceives "objectivity" as a novelty, pragmatism sees it as a relic of the discourse of Enlightenment and proposes to replace it with solidarity. Both approaches analyse difficulties of uncritical applications of this term in the history of philosophy and "avant la lettre" science and the assumption that objectivity is just a modification and another name of this concept which is a necessary condition of any knowledge. The thesis is based on texts of Lorraine Daston, Peter Galison, Richard Rorty, Thomas S. Kuhn and Perez Zagorin.
|
|
|
Atmospheric characteristics of white dwarfs
Krejčová, Kateřina ; Kawka, Adéla (advisor) ; Mayer, Pavel (referee)
We have analyzed the ultraviolet spectra of 40 hydrogen-rich (DA) white dwarfs. These spectra have been obtained with the Far Ultraviolet Spectroscopic Explorer (FUSE) satellite and are publicly available via the MAST FUSE database. We derived the effective temperature and surface gravity by fitting the hydrogen Lyman spectral lines with model spectra. By applying white dwarf evolutionary models, we were able to determine the white dwarf mass, cooling age and absolute magnitude. These then allowed us to determine the distance to each star and its predicted gravitational redshift. We have identified several chemical elements in the white dwarf spectra. We determined the abundances of C, Si, P and S by measuring the equivalent widths of selected lines of these elements. Finally, we studied the spectra of J0623-376 and LM Com in more detail.
|
|
| |
|
|
The Electrical Enterprises of the City of Prague and Avant-garde Films
Krejčová, Kateřina ; PETŘÍČEK, Miroslav (advisor) ; VOJTĚCHOVSKÝ, Miloš (referee)
This paper, based on archival and period media research, tries to describe the relationship between the Electrical Enterprises of the City of Prague and the Czechoslovak avant-garde in the 1920's and 1930's. The Electrical Enterprises of the City of Prague helped to carry out several exceptional film and intermedia projects through their regular advertising contracts, including the film Praha v záři světel (Prague by Night, 1928), Zdeněk Pešánek's light-kinetic sculpture (1930) or the short experimental film Světlo proniká tmou (Light that Penetrates Darkness, 1931). There are also screenplays in the company archives which have never been realized but demonstrate much about the relationship between advertising and art in the interwar period. The author emphasizes the role of the public's general fascination with electrification, artificial light in the modern city and new forms in the collectively shared public space.
|
guest ::
RSS feed.