National Repository of Grey Literature 117 records found  beginprevious45 - 54nextend  jump to record: Search took 0.01 seconds. 
Students' successfulness and strategies when working with a map and factors affecting them
Havelková, Lenka ; Hanus, Martin (advisor) ; Knecht, Petr (referee) ; Bláha, Jan Daniel (referee)
Nowadays, information is increasingly presented in the form of various graphic materials. One of the key means of information visualisation is a map. Maps are complex representations and it is necessary to comprehend several cartographic concepts and to acquire skills and strategies for their efficient use. For this reason, it is important to give maps sufficient attention in the course of education. For the education to be of high quality, it is fundamental to understand the process of the map use and factors affecting this process and its successfulness. Therefore, the general purpose of the dissertation thesis is to develop this understanding. Specifically, the thesis has four main aims. One of them is to identify a map skill level of Czech students while using thematic maps since the popularity of thematic maps is increasing together with the number of cartographic insufficiencies they contain. These insufficiencies can inter alia cause a formation of misconceptions both about the maps and phenomena and regions they display. The second main aim is to identify and describe strategies that students choose to solve tasks which require the use of a thematic map. Additionally, the sub-aim is to understand the influence of chosen factors on the level of map skills as well as on choice and efficiency...
Application of augmented reality in maintenance of production machines
Blaha, Jan ; Kroupa, Jiří (referee) ; Tůma, Zdeněk (advisor)
The master's thesis deals with the design of an augmented reality application for production machine maintenance. The thesis consists of two parts: the theoretical and the design part. The first part of the thesis summarizes the subject matter of augmented reality from a general point of view. It also includes an overview of leading manufacturers. The practical part of the thesis deals with application design. It contains the thought process from the database creation to the final prototype. Several possible solutions are listed, and the most suitable one is selected. The conclusion of the thesis contains a discussion of the results obtained, and practical recommendations for future uses.
Influence of crystalloid and colloid solutions on blood coagulation using the rotational thromboelastometry (ROTEM) method
Binterová, Silvie ; Durila, Miroslav (advisor) ; Vaněk, Tomáš (referee) ; Bláha, Jan (referee)
Fluid resuscitation with crystalloid and colloid solutions is a common treatment in perioperative medicine. However, a variety of unbalanced or balanced solutions is used in clinical practice and there is still a vivid debate going on regarding the selection of optimal fluid with minimal negative effect on coagulation. The goal of the dissertation was to investigate the adverse effect of balanced crystalloids and colloids on whole blood coagulation measured by method of rotational thromboelastometry. In the first phase of the work we had assessed the adverse effect of balanced crystalloid, hydroxyethyl starch and gelatin after dilution of blood with the solution in vitro. Parametrs of EXTEM and FIBTEM tests were evaluated by using rotational thromboelastometry. In the second phase of the work we evaluated the negative effect of infusion solution after dilution in vivo. We had obtained blood samples from 30 patients during knee arthroscopy before and after administration of 500 ml of crystalloid or hydroxyethyl starch or gelatin. Parametrs of EXTEM and FIBTEM tests were evaluated by using rotational thromboelastometry.In compliance with the results of the dissertation, hydroxyethyl starch has the most obvious negative effect on clot formation followed by gelatin and finally by crystalloids. Based on...
Recombinant expression of transcription factor TEAD1.
Řeháková, Jana ; Novák, Petr (advisor) ; Bláha, Jan (referee)
TEAD1 is one of the members of the TEAD transcription factor family. This transcription factor is very important, for example for development of organs. The structure of the entire TEAD1 transcription factor is not now yet known. Nevertheless, structure of two important domains are known. The TEA binding domain, which is important for the binding of the transcription factor TEAD1 to DNA, and the transactivation domain, to which coactivators bind. TEAD1 binds to DNA and upon coactivator bind it affects the transcription of various genes. Genes, that are affected by the transcription factor TEAD1 includes genes regulating proliferation, differentiation and apoptosis of cells. TEAD1 is also the target of the Hippo signalling pathway, which is active in adulthood and prevents abnormal growth of organs. Important for the activity of transcriptional factor TEAD1 are post-translation modifications, such as palmitoylation and phosphorylation. To discover the entire structure of the transcriptional factor TEAD1 and the way it interacts with DNA, the transcriptional factor TEAD1 was prepared recombinationally by expression in cells of Escherichia coli bacteria. Suitable conditions for production of the transcriptional factor TEAD1 were found and the cleavage of the histidine tag by thrombin was performed....
Study of structure and interaction of human lymphocyte receptors
Bláha, Jan
Natural killer (NK) cells are an essential part of immune system, providing self-surveillance of virally infected, stress transformed or cancerous cells. NKR-P1 receptors and their ligands from clec2 gene family represent an alternate missing-self recognition system of NK cells based on interaction of highly related C-type lectin-like receptors. Human NKR-P1 has been described more than twenty years ago but still remains the sole human orthologue of this receptor family, particularly numerous in rodents. On binding to its cognate ligand LLT1, NKR-P1 can relay inhibitory or co-stimulatory signals. Although being interesting targets for their potential role in tumor immune evasion and autoimmunity, nature of their interaction is still unclear. To elucidate the architecture of their interaction, we developed a generally applicable method for recombinant expression of human NKR-P1 and LLT1 and their homologues based on transfection of HEK293S GnTI- cells. Further, we described a stabilizing mutation His176Cys, that enables for expression of highly stable and soluble LLT1. Finally, we have crystallized LLT1 and human NKR-P1 in different glycosylation states both as individuals and in complex. While both structures of LLT1 and NKR-P1 follow the classical C-type lectin-like superfamily fold, contrary to...
The regulation of brown bullhead - \kur{Ameiurus nebulosus} (Lesueur, 1819)
BLÁHA, Jan
The presence of non-native fish species is one of the causes of loss of biodiversity or even degradation of entire ecosystems. The spread of non-native species is often of invasive nature, which is accompanied by a number of direct and indirect effects on the original organisms. These species are often characterized by greater aggressiveness, resistance, growth rate, early sexual maturity period or the presence of anti predatory mechanisms. The expansion of non-native species is mostly and significantly the result of human behaviour. This bachelor thesis is focused on the possibility of regulation of Brown Bullhead or its similar relative Black Bullhead. Brown Bullhead has been present in our country since 1890. The regulation was done by the means of traps. Different kinds of baits were also used in order to maximize the efficiency and selectivity. The data on the actual occurrence of these species in the Czech Republic has been obtained based on the mapping. Historical data need to be confirmed or disproved of and an assessment of its occurrence had to be made as accurately as possible. The thesis also describes other non-native fish species currently present in the waters of the Czech Republic that can affect water ecosystems. The conclusion also contains suggestions of some preventive measures that could be taken to eliminate the possibility of further spread of non-native species of fish in the Czech Republic.
Production of IP camera parts on FDM 3D printer
Blaha, Jan ; Šeniglová, Hana (referee) ; Zemčík, Oskar (advisor)
Bachelor thesis dealing with production of IP camera body, using 3D printing method. This thesis contains two parts. The first one is research part and the second is design part. The first part summarizes the problems in the area of industrial cameras and information about 3D printing method. At the end of the research section, a suitable material is selected for printing on the FDM printer. The design part describes prototype design and production of the selected part. Design includes control calculations and technological design assessment. At the end of the bachelor thesis, there is presentation of technical-economic evaluation and recommendations for the future.

National Repository of Grey Literature : 117 records found   beginprevious45 - 54nextend  jump to record:
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