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Methods of monitoring the profile of carnitine metabolites in urine and use in clinical diagnosis
NOVÁKOVÁ, Martina
This study deals with the profiling of carnitine metabolites in the urine of the patients with breast cancer. L-carnitine is a chemical compound that is very important for metabolism of lipids, and therefore some current research suggests that it could serve as a means for the treatment of cancer affecting various organs or tissues. The theoretical part deals with the general characteristics and a description of the L-carnitine and its effect on the body. This part is divided into chapters that summarize various information about L-carnitine and acylcarnitines, for example the metabolism, different effects in the body including a description of responses to the lack of these substances, or methods that can be used for the detection of L-carnitine levels. Methods of monitoring L-carnitine were described in detail and were compared with previously published scientific studies. The authors of scientific studies show procedures, advantages and disadvantages of the proposed methods for determining L-carnitine and acylcarnitines, that were examined in this work in detail and the method for the determination of carnitine metabolites in urine has been proposed. A brief description of the most using methods for the determination of L-carnitine metabolites by liquid chromatography mass spectroscopy (LC / MS) is done the end of this part. I have been motivated with a positive results of studies and I have used this method for analysis in my work. In the research part of my study, the sample preparation protocol and conditions for the application of the method are described. The samples from the hospital of České Budějovice have been obtained from patients with fresh breast cancer diagnosis. In this part, the treatment of samples prior to detection, the used materials, equipment, chemicals, process and device settings for analysis were described. The method provided chromatograms and mass spectra of the individual substances. Samples of patients were compared with samples of control group of healthy women, which enabled us to observe possible differences. For clarity, the graph was drawn up and it was possible to monitor only slight differences between the levels of carnitine metabolites in women with breast cancer versus the control samples. This difference is not statistically significant, especially because the variability between individuals is higher of an order of magnitude than the actual differences between the two groups. This result is due to the fact that the study has not included additional information on patients such as information about their medications or health style, the overall condition of the body, etc. A detailed study requires a systematic and very time consuming work, which was not the aim of my thesis. Liquid chromatography with hydrophilic interactions method associated with mass detector used for monitoring of L-carnitine and its metabolites profiles gives positive results, is not time consuming, affordable and above all allow suitable for the detection of substances that have been selected for my work.
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Turn to the ordinary in contemporary architectural thinking
Nováková, Martina
The paper seeks to clarify the ambiguous relationship between architecture and ordinariness – more specifically how the architecture has been inspired by the ordinary. The reasoning behind this argument is that there are many European architects in whose work it is possible to find clear and successful tendencies towards the ordinary. These architects are not, however, the first ones to adopt this shift to reality, to ordinariness. The architecture which is explored in this paper is of exceptional quality which typically results from a modern understanding of production, tectonic density and strength of compositional. It also demonstrates strong empathy for its local conditions as well as a confident critical approach to the modern avantgarde. It responds to the desire for purity, truth and often affirms a repressed social and cultural reality. Although the architecture itself engages with banal, everyday and secondary, it must be understood that we are dealing with architecture. The difference is significant; there is no conciliation between architecture and nonarchitecture, between art and non-art. If architecture or art shifts towards the ordinary, it maintains its status as architecture or art; when the ordinary attempts to shift towards architecture or art, it can be at most kitsch. The presence of this boundary is immutable. It can be moved, alienated and obscured but never destroyed.
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Renewal of the cadastre documentation by revision in the cadastre unit Rakvice
Nováková, Martina ; Kutálek, Stanislav (referee) ; Šváb, Tomáš (advisor)
This diploma thesis deals with a renewal of cadaster documentation by revision in a cadastral area Rakvice. This work describes single phases of the renewal and some irregularities, that occurred during the work, including their solutions. The next part of the thesis deals with comparison of a digitalized cadastral map, which came from the revision, and a digital cadastral map, which was created by a new mapping. The appendix contains protocols of processes of transformation and graphic comparison of cadastral maps.
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Big Data analysis in healthcare
Nováková, Martina ; Kučera, Jan (advisor) ; Chlapek, Dušan (referee)
This thesis deals with the analysis of Big Data in healthcare. The aim is to define the term Big Data, to acquaint the reader with data growth in the world and in the health sector. Another objective is to explain the concept of a data expert and to define team members of the data experts team. In following chapters phases of the Big Data analysis according to methodology of EMC2 company are defined and basic technologies for analysing Big Data are described. As beneficial and interesting I consider the part dealing with definition of tasks in which Big Data technologies are already used in healthcare. In the practical part I perform the Big Data analysis task focusing on meteorotropic diseases in which I use real medical and meteorological data. The reader is not only acquainted with the one of recommended methods of analysis and with used statistical models, but also with terms from the field of biometeorology and healthcare. An integral part of the analysis is also information about its limitations, the consultation on results, and conclusions of experts in meteorology and healthcare.
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The internal political development in Kenya since 90s of the 20. century
Nováková, Martina ; Kochan, Jan (advisor) ; Pavlicová, Vendula (referee)
This bachelor thesis provides information about the internal political development of Kenya with an emphasis on the period after the Cold War. The first part of the paper introduces the democratic development of the Sub-saharan Africa since 90s of the 20.century and the conditions influencing democratization. The second chapter focuses on Kenya and its internal political development and explains historical connections and apply the conditions of democratization on Kenya. The third part is dedicated to 2 most important events occured in the last decade - presidential elections held in 2007 and new constitution of kenya since 2010. The last chapter describes current state of the democracy in Kenya and illustrates its future internal political development.
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Relaxing and Stimulating Effects of Music and Dance Therapy for Children.
NOVÁKOVÁ, Martina
Bachelor thesis of "Relaxing and Stimulating Effects of Music and Dance Therapy of Children" has a theoretical and empirical character. The theoretical part deals with musictherapy, its relevance, its historic look, personality types, music therapist and other kinds of expressive therapies, which are related to music-therapy. The next chapter of the bachelor thesis is focused on relaxation, in conjunction with the relaxing effects of the music, that works on the mental and physical health of a person. The chapter about a stimulation is linked to child's play and stimulation effects of music, which also operates on the mental and physical health of a person. In the last issue of the theoretical part is illustrated in dance therapy, which emphasizes the connectedness assumption of the body and mind. The practical part is focused on experimental investigations related with the application of music-dancetherapy program for discharging children. The results of the investigation have proved the feasibility of the music-dancetherapy program for selected children's groups.
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Isolation and study of the properties of recombinant pseudoosmotin
Richterová, K. ; Nováková, Martina ; Antošová, Z. ; Neubauerová, Tereza ; Macková, Martina
Antimicrobial peptides represent new clinical compounds with antimicrobial action and they could participate in solving the problem of ever-increasing resistance of microorganisms to the traditional antibiotics. The aim of this work was to study optimal conditions for expression, isolation and purification of recombinant pseudoosmotin from Escherichia coli BL21 (DE3). Pseudoosmotin was isolated from membrane fraction of bacteria and was purified by the affinity chromatography using Ni-NTA. Isolated pseudoosmotin did not show antimicrobial activity against studied microorganisms.
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Comparsion of usability of freely available mapservers and web map services (WMS) for geodetic work on the Web.
NOVÁKOVÁ, Martina
The aim of this work is to look for freely available map servers and web map services on the Web that are useful for surveying activities, including developement of land adjustments. This thesis deals with theoretical problems of the functioning of map servers, web map services and standards for creating maps. The most famous map servers are described in detail and then compared with the less popular mapservers in the next section. The conclusion then relates to the use maps and assess the suitability of map servers for geodetic purposes and desin of land adjustments. This work aims to determine which map servers are most useful for geodetic purposes.
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