|
|
Kindergarten
Sojka, Petr ; Jelínek, Petr (referee) ; Struhala, Karel (advisor)
The theme of my diploma thesis is processing of project documentation for building a construction of nursery school in Kunštát. Health centre was also designed as a part of the building facility. The capacity of the building are two classrooms in nursery school and two outpatient units. The building is a two-storey building which has basement partly under it. The construction system is bricked from aerated concrete blocks with a ceiling structure made of reinforced concrete prestressed ceiling panels. The perimeter masonry is insulated with an ETICS thermal insulation composite system with mineral wool thermal insulation. The predominant part of the roof of the building is a vegetation extension flat roof.
|
|
| |
|
|
Code switching and code mixing in DVTV - online channel interviews
Morávková, Alena ; Sojka, Pavel (advisor) ; Janovec, Ladislav (referee)
The aim of this thesis is to find out to what extent DVTV Internet TV speakers adhere to Literary Czech in each interview they perform and under which circumstances they do not. We also wanted to contribute to the answers to the question, whether it is appropriate to think about the transition from the current concept of Literary Czech to Standard Czech. During the interview analysis, we monitored the rate of occurrence of the non-standard elements, the influence of the speaker's role in the interview, and selected sociolinguistic factors which have influence on the choice of language code. We compared the results with the research of other journalistic interviews and with the corpus data from the Common Czech. The examined material consisted of 30 interviews from January to February 2016. We found out that some of the interviewed people tended to use Literary Czech, some were speaking non-standard Czech and there were also people whose speech was in terms of the occurrence of Literary and non-standard elements rather mixed. Nevertheless, in general, the character of the texts was rather literary. Based on a comparison of the results with other research, we came to the conclusion that the transition from Literary Czech to Standard Czech would be premature, as the speakers are able to comply with...
|
|
|
Newly-built multipurpose building in Boskovice, execution of superstructure.
Sojka, Petr ; Nečasová, Barbora (referee) ; Kovářová, Barbora (advisor)
The theme of my bachelor thesis is processing technology of rough superstructure of new multi-building owned by Lamex. This is partly double-deck building consisting of storage, administrative and retail space. The subject of my work is processing technology prescription for construction of precast concrete frame and construction of steel fibre reinforced concrete floor. The other parts of this thesis, which with focused on rough superstructure are technical report, construction plan, budget with statement of merit, time schedule, wokers‘ balance, site equipment, safety of workers.
|
|
|
Heterologous expression and isolation of human cytochrome P450 1B1
Sojka, Pavel ; Martínek, Václav (advisor) ; Moserová, Michaela (referee)
Cytochromes P450 are heme enzymes with very broad substrate specificity, they can oxidize tens to hundreds of different substrates including both eobiotics and xenobiotics, but with varying efficiencies and kinetics. They are responsible for the metabolic activation of many carcinogens resulting in formation of reactive intermediates, these reactive species participate in the formation of DNA adducts and also increase of oxidative stress. Eukaryotic cytochromes P450 are membrane bound enzymes found mostly in the endoplasmic reticulum. In vertebrates, they are expressed in a variety of tissues mostly in the liver, but also in kidney, lung, skin and others. The cytochrome P450 1B1 is an inducible enzyme which occurs mainly in the lung and skin. Its expression is induced predominantly by the presence of polycyclic aromatic hydrocarbons, dioxins and heterocyclic amines. The human cytochromes P450 are typically obtained using heterologous expression and isolated as a C-terminally modified hexa-histaq constructs using immobilized metal affinity chromatography. This thesis focuses on effect of C-terminal modifications on activity of human cytochrome P450 1B1. First, the two expression vectors encoding the human form of cytochrome P450 1B1 were prepared, one contained a classical C-terminal hexa-histaq...
|
|
|
Morphological Variation in Spoken and Written Communication of Pupils from the Sixth to the Ninth Grade in the Central Bohemian Dialect Region
Sojka, Pavel ; Janovec, Ladislav (advisor) ; Svobodová, Jana (referee) ; Höflerová, Eva (referee)
TITLE: Morphological Variation in Spoken and Written Communication of Pupils from the Sixth to the Ninth Grade in the Central Bohemian Dialect Region AUTHOR: Pavel Sojka DEPARTMENT: Czech Language Department, Faculty of Education in Prague SUPERVISOR: PhDr. Ladislav Janovec, Ph.D. ABSTRACT This dissertation presents the results of the research in the morphological variation in the spoken and written expression of primary-school pupils (sixth-to-ninth graders) within the central Bohemian dialect region. The impetus behind this research is the long-standing requirement for linguistic examination to pay attention not only to the language of adults, but also to that of pupils and students. From the viewpoint of pedagogy, it establishes the extent to which the speech of children and youth features typical morphological tendencies of contemporary Czech, from the viewpoint of linguistics, it verifies the extent to which schooling manages to fulfill one of the crucial goals of linguistic education, i.e. appropriation of the standardized form of national language. The examined material comprised three specimens: transcriptions from lessons, recordings of a radio show and internet advertisements. The analysis of the specified linguistic material yielded that from the viewpoint of the usage of standard morphology,...
|
|
|
Language of Primary School Pupils
Cmíralová, Hana ; Janovec, Ladislav (advisor) ; Sojka, Pavel (referee)
This work deals with contemporary speech elementary school pupils. The theoretical part of the author accesses the current terminology of unstructured language services, defines the term speech of students in relation to the slang and presents the basic characteristics of speech of pupils and their holders. The material from witch the author builds vocabulary to describe student speech, was acquired through long-term academic research environment. Based on the examination of the recovered materials, the author of several significant conclusions concerning the speech of students. Speech students include specific language statement, which wants pupils to show their unique relationship with the school and the world around them. New words, they create, are a reflection of how the school and, upon it, are experiencing. For the speech of students is typical naming considerable ingenuity, applied in a number of her naming practices. In addition to the traditional derivation is a common distortion and mechanical reduction. Many is the transfer of meaning, especially metaphorical and acceptance of foreign languages. Student vocabulary is highly variable and includes a large number of new and traditional names, but names and fhrases are absolete or do not. This variability pupil vocabulary derives mainly from...
|
|
|
Construction of vectors for heterologous expression of human cytochrome P450 1B1
Sojka, Pavel ; Martínek, Václav (advisor) ; Moserová, Michaela (referee)
The thesis was worked out in Laboratory of Molecular Carcinogenesis and Drug Development, which is focus on study of drug metabolizing enzymes including cytochromes P450. Cytochromes P450 are participating at initial phase of biotransformation of xenobiotics and endogenous substances and metabolism of several endogenous compounds, i.e. steroids. This work is focused on construction of expression vectors, based on the plasmid pET- 22b, suitable for heterologous expression of the human cytochrome P450 1B1. This enzyme is predominantly present in the endoplasmic reticulum of extra-hepatic tissues and its expression is induced by dioxins and polycyclic aromatic hydrocarbons. The human gene for cytochrome P450 1B1 was modified using PCR. The cleavage sites for restriction endonucleases were added to both ends of the gene. Another construct also contained N-terminal histidine tag to facilitate easier purification of the enzyme. Both insert and digested plasmids were verified using the agarose electrophoresis and used for ligation and transformation into competent cells (E. coli DH5. Final steps in construction was, however, not successful, probably due to low yields of DNA fragment extraction from agarose gels. Key words: cytochrome P450 1B1, carcinogenesis, plasmid, heterologous expression [In Czech]
|
|
|
Language of semi-prepared speech
Sojka, Pavel ; Daněk, Jan (referee) ; Janovec, Ladislav (advisor)
Cílem této práce je hláskoslovná a morfologická analýza mluvených textů rozhlasové publicistiky. Při analýze jsme pracovali se třinácti zaznamenanými a přepsanými texty z let 2002-2005. Především jsme se zabývali otázkou, do jaké míry se v tomto typu projevů dodržuje spisovná norma, a výsledky jsme porovnali se závěry pěti dřívějších výzkumů mluvené češtiny. Zjistili jsme, že výskyt nespisovných prvků v našem vzorku je většinou nižší než v dřívějších výzkumech mluvené češtiny. Zároveň se však potvrdilo, že zejména některé nespisovné prvky mají tendenci pronikat i do projevů zřetelně tíhnoucích ke spisovnosti. Analyzované polopřipravené projevy proto hodnotíme jako projevy jazykově smíšené s převahou spisovných prvků.
|
|
|
The Language of Partly Prepared Speech (guides)
Hlaváčková, Klára ; Sojka, Pavel (referee) ; Janovec, Ladislav (advisor)
Professional public show considerable interest in a form of spoken language. The dissertation The Language of Semi-prepared Speeches (Guides in Castles and Chateaux)' has the honour to extend this area of research. The base of this essay is material obtained by field research. Speeches of ten guides in twelve sights were screened by phonetic and phonological analysis. The aim of this analysis was a description of the language in the communication that springs up between visitors of sights and their guides. Furthermore, we are dealing with basic anomalies of literary pronunciation and we are trying to observe some stereotypes in the speech of this profession group. Step by step this dissertation is dealing with vowels, it is observing their quantity (stretching, reducing), quality (open, closed pronunciation, reduction, vowels changes, elision) and prothetic form. Chapters about analysis of consonants, especially their elision, are the next. There is also a passage analysing sentence intonation. The end is dealing with a speech tempo. What is the speech of the surveyed guides like then? The answer is in the content of the dissertation.
|
guest ::
RSS feed.