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Light platinum group metal complexes with a ferrocene N-phosphinoamide
Navrátil, Michal ; Štěpnička, Petr (advisor) ; Gyepes, Róbert (referee)
Title: Light platinum group metal complexes with a ferrocene N-phosphinoamide Author: Bc. Michal Navrátil Department: Department of Inorganic chemistry Supervisor: prof. RNDr. Petr Štěpnička, Ph.D., DSc. Abstract: The aim of this diploma thesis is the preparation of coordination compounds containing ferrocene phosphinoamide FcCONHPPh2 (1, Fc = ferrocenyl), whose preparation was discussed in author's bachelor thesis. Twelve new complexes are described in this thesis, including their characterisation by NMR and infrared spectroscopy, elemental analysis and mass spectrometry. In all cases the crystal structure was determined. Two palladium(II), one rhodium(III) and one ruthenium(II) precursors were used for the preparation of the complexes with each providing three new compounds. A reaction of the precursors with phosphinoamide 1 yielded complexes, in which the phosphinoamide was P-coordinated. This compound was a precursor for the other two complexes. The first one was obtained by a reaction with silver(I) perchlorate producing a cationic P,O-chelate. The other was obtained by a reaction with potassium tert-butoxide resulting in neutral P,O- chelate. All twelve complexes were prepared with optimised yields. Keywords: ferrocene, amides, phosphines, palladium, rhodium, ruthenium, structure elucidation.
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Human glutamate carboxypeptidases II and III
Navrátil, Michal ; Konvalinka, Jan (advisor) ; Obšil, Tomáš (referee) ; Pavlíček, Jiří (referee)
The herein presented Ph.D. dissertation describes kinetic and structural characterization of human glutamate carboxypeptidases II and III (GCPII and GCPIII) using a complete panel of their natural substrates. These enzymes hydrolyze C-terminal glutamate from their substrates. They share 67 % sequence identity and also similar enzymatic activities. This thesis quantitatively compares human GCPII and GCPIII in terms of their ability to hydrolyze the substrates N-acetyl-L-aspartyl-L-glutamate (NAAG), folyl-poly-γ-L-glutamic acids (FolGlun) and β-citryl-L-glutamate (BCG). We demonstrated that GCPIII hydrolyzes its substrates in a metal- dependent manner, that BCG is a specific substrate of GCPIII, and that NAAG and FolGlun are specific substrates of GCPII. We also provide indirect biochemical evidence that GCPIII might feature a heterometallic active-site cluster. Additionally, we characterized the relevance of a surface exosite of GCPII, the arene-binding site (ABS), for the hydrolysis of FolGlun substrates using mutagenesis and enzyme kinetics and showed that polymorphic His475Tyr variant of GCPII hydrolyzes FolGlun substrates with the same kinetic parameters as the wild-type enzyme. Furthermore, this thesis focuses on structural aspects of the substrate specificities of GCPII and GCPIII: we present...
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DEMATEL method and its utilization in multi-criteria decision making
Navrátil, Michal ; Jablonský, Josef (advisor) ; Fiala, Petr (referee)
This thesis is concerned with problematics of multi-criteria decision making, especially method DEMATEL, its modifications and combinations with another methods. There is description of particular steps of this methods and selected theory from various areas, which is used in these methods. In every chapter we can find theoretical explanation of these methods, description of their steps and afterwards these theoretical findings are applied on numerical example. Firstly, the crucial method is introduced. Then this thesis is concerned with its adjustments based, on different approaches, which are often used not only in decision making. In the end, all these findings are aggregated and new method is founded.
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Project Defence Timekeeping System
Navrátil, Michal ; Crha, Adam (referee) ; Růžička, Richard (advisor)
Goal of this thesis is to design and assemble ethernet module for time measuring panel containing watch and stopwatch which will provide network interface to this panel and will power it through ethernet. One of requirements was to develop a control application for microprocessor controlling watch and stopwatch which loads time via this module from the internet and it sets the watch. Stopwatch is controllable via network through control application which was created as part of this thesis. For this purpose there was created a server running on the microprocessor which interacts with control application. Executables of control application are generated for operating systems Windows and Android. It is easy to run it on Linux as well. Contribution of this thesis is possibility to easily track time from the pc or the phone where student and examiner will have a precise information about time.
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Identification of emotional state using speech signal analysis
Navrátil, Michal ; Atassi, Hicham (referee) ; Smékal, Zdeněk (advisor)
The diploma thesis deals with the analysis of human emotional states speaker by the help of analyse speech signals. The thesis has two parts. In the first part, the process of speech generating is described in addition to the description of the commonly used pre-processing methods such as denoising or preemphasis. The first part also deals with the major and minor prosody features, these features are: the fundamental frequency, energy, spectral features and time domain features such as the speech rate. The second part of this thesis deals with a task of emotion recognition from the speech signal. When we accumulate sufficient of the number of recordings emotive state will be able to rekognize emotive state with high probability. All project is prepared for use in real time. The last part of this thesis thesis contains description and results of the experiments made on a large number of speech records.
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The Role of the Christian in Civil Society
NAVRÁTIL, Michal
The work deals with the role of the christian and the Church in civil society focusing especially on the conditions in the Czech republic. The theoretical part defines the term ``civil society{\crqq}, its functions, determining factors and the main time phases of its development. It then goes on to characterize the understanding of man and society from the point of christian social doctrine, the mission of the christian and the Church, and attempts to find the common ground. The practical part of this work describes The Monastery of Divine Mercy in Nové Hrady and how it functions in the civil society of that region.
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