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Redox labelling of nucleic acids for analyzing nucleotide sequences and monitoring DNA-protein interactions
Fojta, Miroslav ; Havran, Luděk ; Horáková Brázdilová, Petra ; Pivoňková, Hana ; Kostečka, Pavel ; Macíčková-Cahová, Hana ; Raindlová, Veronika ; Vrábel, Milan ; Hocek, Michal
Nucleobase labelling of DNA for electrochemical sensing was attained through chemical modification of thymine bases with osmium tetroxide in the presence of nitrogenous ligands, or via enzymatic incorporation of nucleotide conjugates with redox-active moieties using labelled deoxynucleoside triphosphates. DNA hybridization, primer extension and PCR techniques were used for sequence-specific DNA assays. Tail-labelled DNA substrates were applied to monitor DNA binding by tumour suppressor p53 protein.
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Jewellery ( collection of jewellery pieces). Jewellery in the create of natural civilisations.
HORÁKOVÁ, Petra
The following dissertation is presented in two parts. The main part is focused on the collection of twelve jewellery pieces, which I designed and produced myself. In case of decorative objects I tried to find a balance of shapes, structures and materials. The jewellery is made from organic and natural resources which I often find charming and fascinating. The greatest inspiration for me had to be the world`s natural civilisations, which don{\crq}t regard the jewellery pieces just as decoration but for them represent also magical and spiritual meaning. In the theoretic part of my dissertation I focused on the comparison of jewellery in natural civilisations with the primate jewellery of mankind. In this part of my work I searched for the roots of magic and art in general and I discovered the connections with todays remaining natural civilisations {--} magical meaning of jewellery, use of material and meaningful ornaments. Here I also mentioned the use and representation of jewellery. Attempting to clarify the concept of amullet, talisman and fetish. All written text is supported by picture documentation. In the last chapter I summarised the main focus of my dissertation and reflected on the creative process of my work. The last part of my dissertation contains all photo documentation of the creative process.
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The industrial and storage property market
Horáková, Petra ; Heřman, Jan (advisor) ; Zeman, Karel (referee)
The goal of this final essay is to present and evaluate the industrial and storage property market in the Czech Republic, to highlight its strengths and weaknesses, to compare it with the trends in other segments and with international statistics, and to estimate the direction of future development. A significant part deals with the realisation of projects in the form of regenerating brownfields, renewing and re-using damaged territory.
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