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The significance of the analysis of noncholesterol sterols and profile of fatty acids in various pathophysiological states
Vecka, Marek ; Tvrzická, Eva (advisor) ; Zadák, Zdeněk (referee) ; Kazdová, Ludmila (referee)
At present time, there is growing interest in two classes of lipids - sterols and fatty acids, which posses many different roles in living organisms. The concentrations of cholesterol biosynthetic precursors (lathosterol, desmosterol) reflect level of cholesterol biosynthesis, whereas the plasma phytosterol (campesterol, β-sitosterol) concentrations can be used for estimation of fractional absorption of dietary cholesterol. These groups are sometimes called together as noncholesterol sterols. The dissertation thesis dealt with the significance of analysis of noncholesterol sterols and fatty acid profile in various pathophysiological states. The methodology part of the thesis covered some aspects of analysis of these compounds with capillary gas chromatography. We found that for the minor fatty acid analysis, both the type of the column and the software used play an important role. The gas chromatography step contributes by the least part to the total error of the procedure, whereas the effect of other steps (extraction, thin-layer chromatography and methylation process) is more pronounced. The comparison of fatty acid profiles in lipoprotein particles with low and very low density revealed differences, but these differences were highly interindividual. We also found that it is possible to replace the...
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