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3D printed microscopes
Žáková, Veronika ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
Modular microscope building kits allow those interested in microscopy to get a hands-on introduction to the construction of a light microscope. For this reason, it would be appropriate to equip the laboratories of the Institute of Physical Engineering at Brno University of Technology with such a kit. The inspiration can be found in the open source project openUC2, which offers optical kits based on a combination of 3D printed and commercially available optical components. The aim of my work is to use the open source parts database and supplement it with my own designs that would enable the construction of specialized polarization and fluorescence microscopes. The first part of the thesis introduces light microscopy methods and the equipment that is essential for their proper operation. Next, the 3D printing methods that are applicable to the fabrication of the designed parts are summarized. Beyond the openUC2, the design of a Köhler illuminator, a motorized stage and a motorized objective slider based on commonly available parts are introduced. A transmission microscope, a reflective polarizing microscope and a light sheet microscope were designed based on the UC2 kit with my construction modules. The first two have been assembled and tested along with their control software.
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Microscope for alignment of the optical fibers
Hekrlová, Kateřina ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
The demountable splicing of optical fibres uses different types of connectors which ensures accurate position of connected fibres. If the optical fibres are aligned in free space, a view from two perpendicular viewing directions is necessary for a maximum aligning accuracy. The method of direct monitoring of optical fibres provides this possibility however, it is necessary to use two imaging systems. This problem can be solved by a special microscope, which is designed in this thesis. The microscope can visualize the alignment of optical fibres from two mutually perpendicular directions by moving the objective lens and inclined mirror. The diploma thesis also describes the procedure of designing an optical simulation of the connection of optical fibres. Based on it, the microscope is designed, adjusted and tested with various optical fibres.
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3D printed opto-mechanical devices
Šremrová, Vendula ; Dostál, Zbyněk (referee) ; Jákl, Petr (advisor)
Optomechanical components are widely used in many optical experiments. This diploma thesis deals with design and manufacturing optomechanical components using 3D print technology. These are cheaper alternatives of commercial devices. In addition to 3D printed parts, minimum number of other components are used to assemble functional devices. Using simple experimental setups, the manufactured components are evaluated and compared with commercially available ones. The results show that they can be used in applications where high accuracy is not required. The second part is devoted to the design and manufacturing of a polarimeter as a mechanism combining electrical and mechanical components with 3D printed parts. The polarimeter is used to measure some properties of polarized light.
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Micropositioning tracking system
Svoboda, Tomáš ; Šerý, Mojmír (referee) ; Jákl, Petr (advisor)
This bachelor's thesis deals with the design and construction of a micro positioning device that will monitor the movement of a robotic mouse in a defined space with the help of a camera. The main task will be to assemble a moving platform, the aim of which will be to be in constant contact with the robot below it and to copy its 2D trajectory. The device will then be applied to a live mouse for in vivo holographic endoscopy experiments.
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Wavefront sensor
Škaroupka, David ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
When the light passes through optical elements it can cause deformation of wavefront light due to the damage of materials and optical aberrations. The deformation leads to unprecise imaging and deformation of an image. The diploma work mentions wavefront sensing techniques which are important for determination inappropriate errors of optical elements and the specification of their properties. The purpose of the master's thesis is to suggest and investigate wavefront sensing methods after the light passes through the optical element while using the digital micromirror device. The work deals with the topic of wavefront aberrations and geometric optical defects of optical elements. Different kinds of commercial products determined for wavefront sensing are described too.
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SMV-2019-67: Vector mode
Jákl, Petr ; Zemánek, Pavel
Preparation of experimental apparatus and control software for photopolymerization of nanostructures in vector mode. The nanostructures are created by continuous movement of the sample along a given trajectory with the laser beam switched on.
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Development of Biophysical Interpretation of Quantitative Phase Image Data
Křížová, Aneta ; Jákl, Petr (referee) ; Vomastek, Tomáš (referee) ; Chmelík, Radim (advisor)
This doctoral thesis deals with biophysical interpretation of quantitative phase imaging (QPI) gained with coherence-controlled holographic microscope (CCHM). In the first part methods evaluating information from QPI such as analysis of shape and dynamical characteristics of segmented objects as well as evaluation of the phase information itself are described. In addition, a method of dynamic phase differences (DPD) is designed to allow more detailed monitoring of cell mass translocations. All of these methods are used in biological applications. In an extensive study of various types of cell death, QPI information is compared with flow cytometry data, and preferably a combination of QPI and fluorescence microscopy is used. The DPD method is used to study mass translocations inside the cell during osmotic events. The simplified DPD method is applied to investigate the mechanism of tumor cell movement in collagen gels.
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Coherence Controlled Holographic Microscope with the digital optics
Vavřinová, Jana ; Jákl, Petr (referee) ; Dostál, Zbyněk (advisor)
The Digital Micromirror Device (DMD) technology has been developed especially for Digital Light Processing projectors, which allow the image projection. After this succesful implementation, and thanks to the commercial availibility and low initial cost of the DMD chip, a wide range of other applications became possible. Besides, it may be used in microscopy as a spatial light modulator. For example in Coherence-Controlled Holographic Microscope (CCHM) that finds its use especially for imaging and measurement of live-cell dynamic processes. The DMD chip placed in the illumination part of CCHM allows for broadening the application possibilities. Namely it could be different illumination mode experiments or tomographic applications. The master's thesis deals with the optical design of CCHM with digital optics, i. e. DMD chip. The selection of optical elements for CCHM, the experimental verification of the imaging setup and the process of designing the illumination part are described in detail. In the end, the analysis of different designs for illumination setup with the digital optics in object arm is carried out and the results are compared.
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