|
|
Immunologic profile of experimental autoimmune encephalomyelitis
Novosádová, Iva ; Fišerová, Anna (advisor) ; Zajícová, Alena (referee)
5 Anglický abstrakt Experimental autoimmune encephalomyelitis (EAE) is widely accepted as a murine model of human multiple sclerosis autoimmune disease. Murine EAE is usually actively induced by immunization with a suitable myelin antigen. Following immunization, CD4+ T helper lymphocytes Th1 and Th17 accumulate in the nervous tissue and via the production of cytokines, they mediate an inflammatory reaction and the subsequent destruction of myelin. The main goal of this study was the induction of EAE with clinically observable symptoms and the observation of changes in the counts and phenotypes of cells, mainly NK and T cells. NK cells express a wide range of inhibitory and activation receptors from the C-lectin-like receptor superfamily. The specific ligand of the activating NKR-P1C isoform is still unknown and thus this receptor's involvement in EAE was also observed. Another goal was the use of medication with regard to the disease progress improvement. For the purposes of this study, two inbred murine strains with distinct NKR-P1 surface expression were used - the SJL/J strain (expressing inhibitory NKR-P1B) and C57BL/6 (expression activating NKR-P1C). SJL mice elicited a relapse-remitting of EAE, while C57BL/6 had chronic EAE. Both mouse strains exerted changes in the counts of NK...
|
|
|
Immunological profile of patients with multiple sclerosis
Šubjak, David ; Fišerová, Anna (advisor) ; Růžičková, Šárka (referee)
Immunological profile of multiple sclerosis patients Abstrakt Multiple sclerosis is an autoimmune neurodegenerative disease affecting predominantly the white matter of the CNS and the spinal cord. The mechanism of disease progression is not yet fully understood. In this study we focused on a comparison of selected immunological markers between patients with multiple sclerosis who were naïve newly diagnosed, subsequently treated with Avonex (IFNβ1a) and healthy donors. The T cells (particulary cytotoxic CD8+ T cells) are the major population involved in pathogenesis of MS causing the demyelization of axons. Subpopulation of CD161+ Th cells has a potential to be very important in this process. We focused on the role of NK cells phenotype and function in autoimmune response of patients and their changes during the therapeutic intervention. Using flow cytometry we analyzed the distribution of NK, NKT, T cells and monocytes with special regard to the expression of CD161 and NKG2D molecules on their surface. We observed increase counts of CD161+ cells in subpopulations NK CD56bright , NK CD56dim , Th, Tc CD8bright , Tc CD8dim and decrease counts of NKG2D+ cells in subpopulations NK CD56bright , NK CD56dim , NKT, Th, Tc CD8bright , Tc CD8dim and monocytes. The decreased cytotoxic activity of NK cells in naïve MS...
|
|
|
NKR-P1C receptor-carbohydrate interaction contributes to antitumor immune response via activation of NK and B cells
Hulíková, Katarína ; Fišerová, Anna (advisor) ; Kročová, Zuzana (referee) ; Turánek, Jaroslav (referee)
Lectin - saccharide interactions and the involved receptors are currently intensively studied for their important role in antimicrobial as well as antitumor immunity. The major cell types participating in carbohydrate recognition are NK, NKT, and B cells. The differentiation of B lymphocytes could be induced by activated NK cells via direct intercellular contact and/or IFN-γ release. Our research is focused on NK cell receptors of C-type lectin-like family recognizing carbohydrate epitopes of glycoproteins. Synthetic glycoconjugates with terminal N- acetyl-D-glucosamines on polyamidoamine (GN8P) or calix[4]arene (GN4C) scaffold used in this study, exerted the highest binding affinity to activating isoforms of rodent NKR-P1 (A and C) receptor. The aim of the presented dissertation thesis was to elucidate, how Nkr-p1c gene divergence, between C57BL/6 (NK1.1-positive, NKR- P1CB6) and BALB/c (NK1.1-negative, NKR-P1CBALB/c) mouse strains, could affect NK cell activation and subsequent triggering of B lymphocyte effector functions using GN8P and GN4C as NKR-P1C prototype ligands. We demonstrated, that GN8P increased mRNA expression for NKR-P1C receptor, IFN-γ synthesis and lytic activity of NK cells, antigen-specific (anti-KLH, anti-DNP, and anti-B16F10) IgG (particularly IgG2a) formation, number of...
|
|
|
Impact of NKR-P1 polymorphism on Ly49 receptors expression in hybrid mouse strains (C57BL/6 x Balb/c, F10-12)
Holubová, Martina ; Fišerová, Anna (advisor) ; Vomastek, Tomáš (referee)
Impact of NKR-P1 polymorphism on Ly49 receptors expression in hybrid mouse strains (C57BL/6 x Balb/c, F10-12) Abstract Natural killer (NK) cells constitute the subpopulation of large granular lymphocytes which mediate spontaneous immune response against infected, transformed or allogeneic cells and thus represent an important component of the innate immunity. NK cells express a wide repertoir of surface receptors which can be either activating or inhibitory and which mediate NK cell recognition and regulation of cytolytic activity. NKR-P1 and Ly49 receptor families belong to the most important murine NK receptors. Both NKR-P1 and Ly49 families are members of C-type lectin-like superfamily of receptors encoded by natural killer gene complex (NKC) on chromosome 6 and include both activating and inhibitory members. The aim of this diploma thesis was to elucidate the impact of Nkr-p1c gene divergence on Ly49 receptors expression and to find out whether the Ly49 and Nkr-p1 gene clusters (which are localized on opposite ends of NKC) are inherited independently or whether the NKC domain is inherited as a complex. The second research interest was to illustrate the influence of the above mentioned divergence on cytotoxic activity of NK cells and tumor growth. In this study, inbred mouse strains C57BL/6 and Balb/c...
|
|
| |
|
|
NEW ON THE „OLD“ – Brno, Bratislavská - Stará Corner
Fišerová, Anna ; Pospíšil, Zdeněk (referee) ; Boháč, Ivo (advisor)
Designed area is located in Brno - Zabrdovice, in the corner of streets Stara and Bratislavska . The surrounding area has a distinctive character of urban residential blocks. Character of the surrounding objects are not uniform , there are however mostly historic buildings, on the other hand one of the modern is on the Bratislavska street - Radost Theatre. The proposed building has two underground and 7 above-ground floors. The above-ground part of the building consists of 3 masses . The first mass - parter – has increased height because of commercial and restaurant function and also due to connecion with neighbour building – Police CR. The second mass (2nd - 6.NP ) is formed from part of the administrative area and then the vast majority of housing units . Mass 7.NP recedes from the street line - height roughly corresponds to the ridge of the Police oCR . There arise four atypical housing units, of which two have glass atriums , and all have south or east oriented terraces with attractive views of the city of Brno . The corner of the ground floor is open and forms the main entrance to the courtyard, where we can find a shopping gallery , outdoor cafe seating and access to the administrative part of the building . The result was a distinctive public space , which is used only during the day . At night , this part with respect to security and peace is closed for the night . The building offers 118 parking places on two underground levels, a further 2,865 m2 office and commercial space and 44 residential units.
|
|
|
Residence of Leopold Vilém Kolovrat-Krakovský at the turn of the 18th and 19th century in the light of surviving equipments
FIŠEROVÁ, Anna
This Bachelor´s thesis is focused on the Leopold Vilém Kolovrat-Krakovský´s aristocratic baroque residence which is situated in the Hroby. The main objective of this thesis is reconstruction of the interior layout and material equipment of the seat at the turn of the 18th and 19th century. Like source of knowledge of the material culture of the seat there were used surviving castle inventory which were created in 1795 and 1803. The object of the interest is the characteristic of individual rooms of the seat. This work is also focused on symbolic meaning of the decoration of the interior and objects, which were reflected in world of the thought and value ladder of Leopold Vilém Kolovrat-Krakovský and his family. There is paid special attention to furniture and extraordinary collection of cookware which reflect changing period trends.
|
|
| |
|
| |
|
| |
guest ::
RSS feed.