|
|
Potentiometric determination of ibuprofen.
Šrámková, Ivana ; Kastner, Petr (advisor) ; Kučera, Radim (referee)
Potentiometric determination of ibuprofen Thesis Ivana Šrámková A development of a selective electrode for potentiometric determination of ibuprofen in pharmaceutical formulations was aimed in this work. Several sensors were constructed. Their response to the ibuprofen was under study. It was revealed that a choice of appropriate membrane components as well as the molar ratio of them is of high importance for an ibuprofen-selective sensor. Some of the tested sensor's response is close to the Nernstian response. The best results were achieved using HP-β-CD with the function of an ionophore, 2- phenyl-2-nitrophenyl ether as a solvent mediator and tetrahexylammonium bromide as lipophilic specie. It was found that one of the evaluated electrodes is potentially iodide- selective. The ibuprofen-selective electrode with the best features is still under study.
|
|
| |
|
| |
|
|
Determination of clotrimazol and benzylalcohol in vaginal cream by means of HPLC
Chmurová, Klára ; Kastner, Petr (advisor) ; Mokrý, Milan (referee)
HPLC determination of clotrimazol and benzyl alcohol in vaginal cream Academic dissertation Klára Chmurová Charles University in Prague, Pharmaceutical Faculty in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control The task was to find out a method of simultaneous determination of clotrimazol and benzylalcohol in a vaginal cream. We were looking for proper modification of the pharmaceutical preparation (vaginal cream), then optimization of chromatographic conditions and validation of this HPLC method, too. The best results were obtained with DISCOVERY® HS F5, 5 µm, 4,5 × 150 mm together with the pre-column DISCOVERY® HS C18, 5 µm, 2,1 × 20 mm, mixture of acetonitril and buffer (K2HPO4 0,05mol/l, pH = 6,1 set up by 85% H3PO4) 60 : 40 (v/v) was used as the mobile phase, flow rate was 1 ml/min. Detection was realized by UV detector with wave length 223 nm. Time of analysis was shorter than 10 min.
|
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
guest ::
RSS feed.