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Diagnostics of toxoplasmosis
Bajajová, Kateřina ; Voxová, Barbora (advisor) ; Čermáková, Zuzana (referee)
CHARLES UNIVERSITY IN PRAGUE Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Bc. Kateřina Bajajová Supervisor: PharmDr. Barbora Voxová Title of diploma thesis: Diagnostics of toxoplasmosis Worldwide widespread parasite Toxoplasma gondii (T. gondii) is an agent of toxoplasmosis infection. This infection is the most widespread antropozoona and pertain to the most frequent infectious disease, because it affects about one third of population in its latent form. For immunocompetent people infection is not a risk, its progress is asymptomatic and it causes lifelong latent infection. On the other hand for some of patients means toxoplasmosis weighty medical problem. There are mainly people with immunodeficiency after reactivation of latent form of infection, the primary infection in pregnant women, the infection is transmitted from mother to fetus and newborns with the congenital form of toxoplasmosis. The disease can has in these cases very serious consequences and fatal process. The T. gondii infection can be diagnosed by several methods. Methods are divided into direct, aimed at finding a microbe in biological material, and indirect methods, in which serologically show antibodies against infectious agens. The direct methods include histological...
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Comparison of results of two methods for laboratory diagnosing lyme borreliosis- PCR method and ELISA
Vargová, Radka ; Křivčíková, Lucie (advisor) ; Čermáková, Zuzana (referee)
Bc. Radka Vargová Comparison of results of two methods for laboratory diagnosing lyme borreliosis - PCR methods and ELISA Diploma thesis Charles University in Prague, Facutly of Pharmacy in Hradec Králové Specialist in laboratory methods At present time the Lyme disease is relatively wide-spread affection. Its agent is spirochete Borrelia burgdorferi sensu lato and its most eminent vector in Europe is the tick Ixodes ricinus. In the theoretical part of my thesis I decribed the basic characteristics of the genus Borrelia, its antigenic structure, epidemology and pathogenesis, disease vectors, clinical sypmtoms of the infection and possibilities of laboratory diagnostics. My thesis is aimed at comparison of dignostics of Lyme disease by using serological method ELISA and using method PCR. It was used 113 anonymized samples of patients for analyse. The samples were examined for the presence of the antibody against Borrelia in classes IgG and IgM and by using real-time PCR method they were examined for the presence of DNA pathogenic Borrelia. The experimental part of the thesis describes realization of the methods, which we have used for diagnostics of Lyme disease. In the results part is claimed that we noticed only one positive result within detection of Borrelia DNA (total positivity 0,88%). The...
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The Comparison between Health Care Reform of W. Clinton and Health Care Reform of B. Obama
Čermáková, Zuzana ; Kozák, Kryštof (advisor) ; Sehnálková, Jana (referee)
Bachelor thesis The Comparison between Health Care Reform of W. Clinton and Health Care Reform of B. Obama deals with the health care system of the United States of America. The aim is to explore the mechanisms for securing affordable health coverage of the reform proposals of the last two Democratic presidents of the USA, The National Health Security Act and The Patient Protection and Affordable Care Act. Despite a lapse of more than fifteen years the goals of proposals were the same. Both presidents called for reducing the federal deficit and the number of uninsured Americans. Analysis of the bills shows there are many similarities in the mechanisms for ensuring health insurance. Even if President Obama adopted a more conservative approach than President Clinton, both reform proposals were based on the system of managed competition and on the state intervention.
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Awareness of public about risks of lyme borreliosis
Kafková, Martina ; Čermáková, Zuzana (advisor) ; Skála, Bohumil (referee) ; Košťálová, Jaroslava (referee)
The bachelor's work deals with clinical picture, pathogenesis and diagnostics of Lyme Borreliosis. It is aimed at a cycle of a tick, its ability to transfer the infection to a human organism and also geographical spread within the Czech Republic territory. It emphasizes that the only effective method against the infection of the Lyme disease is prevention, first of all protection against ticks and their timely and proper removal from the body. The main part of work is formed by a quantitative exploratory research of awareness in public about the risks of borreliosis. It is aimed at ways how people protect themselves against ticks and how they handle them during removing. It probes their knowledgeableness about this infection. Examined sample is formed by randomly chosen respondents of various age in the region of eastern Bohemia.
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Electron microscopy in the diagnosis of Lyme borreliosis
Štemberková, Radka ; Čermáková, Zuzana (advisor) ; Ryšková, Olga (referee)
The aim of this thesis was to compare the data from the examination of biological material (liquor, blood) by electron microscopy and from serological testing in order describe the course of the Lyme disease. 117 patients participated in the study from Faculty hospital in Hradec Kralove. Transmission electron microscopy results were compared with serological methods. The results of this thesis showed that Lyme disease is characterized by huge variability, heterogeneity of laboratory results. In conclusion, the diagnosis of Lyme disease is very complicated despite the fact that sophisticated methods are used.
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Internal Migration in PRC: function, role and impact on Chinese economy
Čermáková, Zuzana ; Stuchlíková, Zuzana (advisor) ; Procházková Ilinitchi, Cristina (referee)
The purpose of this thesis is to analyze the process of internal migration in Peoples' Republic of China. It focuses on low-skilled rural migrant workers, their overall characteristics, role in the economy and their status within Chinese society. This analysis aims to prove that despite the fact that rural migrants represent an essential element of Chinese economy, and have contributed by a great deal to China's economic take-off, they are still a very discriminated group of Chinese society and are constantly facing violations of their rights.
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A Child in Foster Care and its Preschool
ČERMÁKOVÁ, Zuzana
Annotation My bachelor thesis focuses on the theme of the substitute family care and the child from the substitute family care in the kindergarten. The theoretical part introduces information from the history of the substitute family care. Further I show the basic types of the substitute family care and the applicants´ duties. Finally there is determined the cooperation of the kindergarten and the parents with the children from the substitute family care on the basis of the framework educational program for pre-school education. The practical part of my bachelor thesis includes resultant information which is provided by the parents with the children from the substitute family care. Information is oriented on negative and positive aspects of the kindergarten attendance by the children from the substitute family care. I received this information thanks to the interview with the parents. Last but not least this part offers the information obtained from the kindergarten teachers, who discuss the experience of the children from the substitute family care.
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